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:: Volume 22, Issue 3 (10-2020) ::
J Gorgan Univ Med Sci 2020, 22(3): 65-72 Back to browse issues page
Effect of Lactobacillus paracasei probiotic in Parkinson’s male rats
Kimia Khandestan1 , Bahareh Pakpour 2, Maryam Tajabadi Ebrahimi3
1- M.Sc Student in Microbilogy, Department of Biology, Islamic Azad University, Central Tehran branch, Tehran, Iran
2- Assistant Professor, Department of Biology, Islamic Azad University, Central Tehran branch, Tehran, Iran , b_pakpour@yahoo.com
3- Associate Professor, Department of Biology, Islamic Azad University, Central Tehran branch, Tehran, Iran
Abstract:   (8194 Views)
Background and Objective: Parkinson's disease is a neurological disorder caused by the loss of dopaminergic neurons in pars compacta of substantial nigra. The most important symptoms of this disease include slow motion, lack of movement, tremor and imbalance. This study was done to determine the effect of probiotic Lactobacillus paracasei on motor disorders caused by injection of 6-hydroxy dopamine in Parkinson's model in male rats.
Methods: In this experimental study, 48 male rats were randomly allocated into six groups. To create an animal model of Parkinson's disease, a black body component in male rats was injected 6-hydroxy dopamine (6-OHDA) (0.5 mg/kg) unilaterally by stereotaxic apparatus. After 3 weeks of recovery, to evaluation of degradation rate, animals were received apomorphine, intraperitoneally. In the first experiment, rats were randomly divided into Sham and Parkinson's (injured) groups, the Sham group were received only water and food, and the injured group received 6-hydroxy-dopamine. In the second experiment, male rats were divided into 4 groups of 8 rats, each of the 4 groups was injected 6-hydroxy-dopamine and 21 days later, they were tested for assay of apomorphine. The results were recorded, then for 28 days, the first group was taken as control. The second group of saline, the third group of milk, the fourth group of milk and probiotic were gavaged. At the end of day 28, once again, each group was subjected to rotation of apomorphine test and the rotations were recorded.
Results: The behavioral analysis of saline, milk, milk plus probiotics were indicated that the group receiving probiotic Lactobacillus paracasei plus milk was significantly less apomorphine rotation test than the saline group (P<0.05).
Conclusion: It seems that probiotic Lactobacillus paracasei can reduces Parkinson’s disease symptoms.
Keywords: Parkinson Disease [MeSH], Lactobacillus paracasei [MeSH], Probiotics [MeSH], Rat [MeSH]
Article ID: Vol22-39
Full-Text [PDF 346 kb]   (9931 Downloads)    
Type of Study: Original Articles | Subject: Neurosciences
References
1. Avenali M, Tassorelli C, De Icco R, Perrotta A, Serrao M, Fresia M, et al. Pain processing in atypical Parkinsonisms and Parkinson disease: A comparative neurophysiological study. Clin Neurophysiol. 2017 Oct; 128(10): 1978-84. DOI: 10.1016/j.clinph.2017.06.257
2. Umbrello G, Esposito S. Microbiota and neurologic diseases: potential effects of probiotics. J Transl Med. 2016; 14: 298. DOI: 10.1186/s12967-016-1058-7
3. Araya AV, Atwater I, Navia MA, Jeffs S. Evaluation of insulin resistance in two kinds of South American camelids: llamas and alpacas. Comp Med. 2000 Oct; 50(5): 490-94.
4. Jafari E, Vahedi H, Merat S, Momtahen S, Riahi A. Therapeutic effects, tolerability and safety of a multi-strain probiotic in Iranian adults with irritable bowel syndrome and bloating. Arch Iran Med. 2014 Jul; 17(7): 466-70.
5. Jafari M, Mortazavian AM, Hosseini H, Safaei F, Mousavi Khaneghah A, Sant'Ana AS. Probiotic Bacillus: Fate during sausage processing and storage and influence of different culturing conditions on recovery of their spores. Food Research International. 2017 May; 95L 46-51. https://doi.org/10.1016/j.foodres.2017.03.001
6. Salehizadeh M, Modarressi MH, Mousavi SN, Ebrahimi MT. Effects of probiotic lactic acid bacteria on growth performance, carcass characteristics, hematological indices, humoral immunity, and IGF-I gene expression in broiler chicken. Trop Anim Health Prod. 2019; 51(8): 2279-86. DOI: 10.1007/s11250-019-01935-w
7. Noohi N, Ebrahimipour G, Rohani M, Talebi M, Pourshafie MR. Evaluation of potential probiotic characteristics and antibacterial effects of strains of Pediococcus species isolated from broiler chickens. Br Poult Sci. 2016 Jun; 57(3): 317-23. DOI: 10.1080/00071668.2016.1169247
8. Sarkar A, Lehto SM, Harty S, Dinan TG, Cryan JF, Burnet PWJ. Psychobiotics and the Manipulation of Bacteria-Gut-Brain Signals. Trends Neurosci. 2016 Nov; 39(11): 763-81. DOI: 10.1016/j.tins.2016.09.002
9. Amara AA, Shibl A. Role of Probiotics in health improvement, infection control and disease treatment and management. Saudi Pharm J. 2015 Apr; 23(2): 107-14. DOI: 10.1016/j.jsps.2013.07.001
10. Wang HX, Wang YP. Gut Microbiota-brain Axis. Chin Med J (Engl). 2016 Oct; 129(19): 2373-80. DOI: 10.4103/0366-6999.190667
11. Paxinos G, Watson CR, Emson PC. AChE-stained horizontal sections of the rat brain in stereotaxic coordinates. J Neurosci Methods. 1980 Dec; 3(2): 129-49. DOI: 10.1016/0165-0270(80)90021-7
12. Badehnoosh B, Karamali M, Zarrati M, Jamilian M, Bahmani F, Tajabadi-Ebrahimi M, et al. The effects of probiotic supplementation on biomarkers of inflammation, oxidative stress and pregnancy outcomes in gestational diabetes: randomized controlled trial. J Matern Fetal Neonatal Med. 2018 May; 31(9): 1128-36. DOI: 10.1080/14767058.2017.1310193
13. Fujita M, Nishino H, Kumazaki M, Shimada S, Tohyama M, Nishimura T. Expression of dopamine transporter mRNA and its binding site in fetal nigral cells transplanted into the striatum of 6-OHDA lesioned rat. Molecular Brain Research. 1996 Jul; 39(1-2): 127-36. https://doi.org/10.1016/0169-328X(96)00018-6
14. Ungerstedt U. 6-Hydroxy-dopamine induced degeneration of central monoamine neurons. Eur J Pharmacol. 1968 Dec; 5(1): 107-110. https://doi.org/10.1016/0014-2999(68)90164-7
15. Puelles L, Verney C. Early neuromeric distribution of tyrosine-hydroxylase-immunoreactive neurons in human embryos. J Comp Neurol. 1998 May; 394(3): 283-308. DOI: 10.1002/(sici)1096-9861(19980511)394:3<283::aid-cne2>3.0.co;2-y
16. Jakesevic M, Aaby K, Borge GIA, Jeppsson B, Ahrné S, Molin G. Antioxidative protection of dietary bilberry, chokeberry and Lactobacillus plantarum HEAL19 in mice subjected to intestinal oxidative stress by ischemia-reperfusion BMC Complement Altern Med. 2011; 11: 8. DOI: 10.1186/1472-6882-11-8
17. Kaizu H, Sasaki M, Nakajima H, Suzuki Y. Effect of antioxidative lactic acid bacteria on rats fed a diet deficient in vitamin E. J Dairy Sci. 1993 Sep; 76(9): 2493-99. https://doi.org/10.3168/jds.S0022-0302(93)77584-0
18. Pakpour B, Ahmadi S, Nayer-Nouri T, Oryan S, Zarrindast MR. Inhibitory avoidance memory deficit induced by scopolamine: interaction with glutamatergic system in the nucleus accumbens. Behavioural Pharmacology. 2010 Dec; 21(8): 719-26. DOI: 10.1097/fbp.0b013e32833fa785
19. Zarrindast MR, Ownegh V, Rezayof A, Ownegh F. The involvement of dorsal hippocampus in dextromethorphan-induced state-dependent learning in mice. Pharmacology Biochemistry and Behavior. 2014 Jan; 116: 90-95. https://doi.org/10.1016/j.pbb.2013.11.015
20. Pfeiffer RF. Gastrointestinal dysfunction in Parkinson's disease. Parkinsonism Relat Disord. 2011 Jan; 17(1): 10-15. DOI: 10.1016/j.parkreldis.2010.08.003
21. Zarrindast MR, Farahvash H. Effects of GABA-ergic drugs on penile erection induced by apomorphine in rats. Psychopharmacology. 1994 Jun; 115(1-2): 249-53.
22. Mulak A, Bonaz B. Brain-gut-microbiota axis in Parkinson's disease. World J Gastroenterol. 2015 Oct; 21(37): 10609-20. DOI: 10.3748/wjg.v21.i37.10609
23. Zarrindast MR, Khakpai F. The Modulatory Role of Dopamine in Anxiety-like Behavior. Arch Iran Med. 2015; 18(9): 591-603.
24. Piri M, Rostampour M, Nasehi M, Zarrindast MR. Blockade of the dorsal hippocampal dopamine D1 receptors inhibits the scopolamine-induced state-dependent learning in rats. Neuroscience. 2013 Nov; 252: 460-67. https://doi.org/10.1016/j.neuroscience.2013.08.003
25. Nasehi M, Hajian M, Ebrahimi-Ghiri M, Zarrindast MR. Role of the basolateral amygdala dopamine receptors in arachidonylcyclopropylamide-induced fear learning deficits. Psychopharmacology (Berl). 2016 Jan; 233(2): 213-24. DOI: 10.1007/s00213-015-4096-6
26. Le Heron C, Kolling N, Plant O, Kienast A, Janska R, Siang Ang Y, et al. Dopamine Modulates Dynamic Decision-Making during Foraging. J Neurosci. 2020; 40(27): 5273-82. DOI: 10.1523/JNEUROSCI.2586-19.2020
27. Haddad D, Nakamura K. Understanding the susceptibility of dopamine neurons to mitochondrial stressors in Parkinson’s disease. FEBS Lett. 2015 Dec; 589(24): 3702-13. DOI: 10.1016/j.febslet.2015.10.021
28. Burke WJ. Beating a dead horse: dopamine and Parkinson disease. Neurology. 2008; 70(22): 2087. DOI: 10.1212/01.wnl.0000315886.20986.94
29. Yakushev IY, Dupont E, Buchholz HG, Tillmanns J, Debus F, Cumming P, et al. In vivo imaging of dopamine receptors in a model of temporal lobe epilepsy. Epilepsia. 2010 Mar; 51(3): 415-22. DOI: 10.1111/j.1528-1167.2009.02272.x
30. Roghani M, Behzadi G. Neuroprotective effect of vitamin E on the early model of Parkinson's disease in rat: behavioral and histochemical evidence. Brain Res. 2001 Feb; 892(1): 211-17. DOI: 10.1016/s0006-8993(00)03296-0
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Khandestan K, Pakpour B, Tajabadi Ebrahimi M. Effect of Lactobacillus paracasei probiotic in Parkinson’s male rats. J Gorgan Univ Med Sci 2020; 22 (3) :65-72
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Volume 22, Issue 3 (10-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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