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Effect of Morin on Expression of Biofilm Gene of Pseudomonas aeruginosa Isolated from Burn Wounds
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Samin Babazadeh1    , Kumarss Amini * 2, Mahsa Kavousi3 |
1- M.Sc in Microbiology, Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Associate Professor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran. , dr_kumarss_amini@yahoo.com
3- Assistant Professor, Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran. |
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Abstract: (3805 Views) |
Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen that is a major cause of mortality in immunocompromised patients. One of the most important mechanisms of resistance of this bacterium is biofilm formation. The aim of this study was done to determine the Effect of Morin on Expression of Biofilm Gene of Pseudomonas aeruginosa Isolated from burn wounds by Real time PCR.
Methods: In this descriptive-analytic study 60 sample were collected from burn wounds of patients admitted to the hospitals in Tehran, Iran. Samples were identified by using biochemical methods. The DNA of the isolates was extracted and then antimicrobial activity of morin analyzed by microbroth dilution assay. The presence of biofilm production genes was investigated by PCR. Finally, the expression of lasI gene in combination with Sub-MIC concentration of morin in biofilm-producing bacteria was evaluated using Real time PCR.
Results: From 60 samples that analyzed by Multiplex-PCR, 12 (20%) Pseudomonas aeruginosa strains were isolated in which 12 isolates (100%) were carried lasI and lasR, genes, respectively. 3 isolates (25%) were carried rhlI gene. Sub-MIC concentration of morin in biofilm-producing bacteria reduced lasI gene expression in Pseudomonas aeruginosa.
Conclusion: Morin has significant efficacy on Pseudomonas aeruginosa and could be a good alternative for treatment of antibiotic resistant isolates. |
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Keywords: Pseudomonas aeruginosa [MeSH], Biofilms [MeSH], Morin [MeSH], Real time PCR [MeSH], Drug Resistance [MeSH]
Article ID: Vol23-55 |
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Full-Text [PDF 583 kb]
(15796 Downloads)
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Type of Study: Original Articles |
Subject:
Microbiology
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1. Rasamiravaka T, Labtani Q, Duez P, El Jaziri M. The formation of biofilms by Pseudomonas aeruginosa: a review of the natural and synthetic compounds interfering with control mechanisms. Biomed Res Int. 2015; 2015: 759348. DOI: 10.1155/2015/759348 [ DOI] [ PubMed] 2. Bodelón G, Montes-García V, López-Puente V, Hill EH, Hamon C, Sanz-Ortiz MN, et al. Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering. Nat Mater. 2016 Nov; 15(11): 1203-11. DOI: 10.1038/nmat4720 [ DOI] [ PubMed] 3. Rampioni G, Schuster M, Greenberg EP, Zennaro E, Leoni L. Contribution of the RsaL global regulator to Pseudomonas aeruginosa virulence and biofilm formation. FEMS Microbiol Lett. 2009 Dec; 301(2): 210-17. DOI: 10.1111/j.1574-6968.2009.01817.x [ DOI] [ PubMed] 4. Kiaei S, Moradi M, Hosseini Nave H, Hashemizadeh Z, Taati-Moghadam M, Kalantar-Neyestanaki D. Emergence of co-existence of blaNDM with rmtC and qnrB genes in clinical carbapenem-resistant Klebsiella pneumoniae isolates in burning center from southeast of Iran. Folia Microbiol (Praha). 2019 Jan; 64(1): 55-62. DOI: 10.1007/s12223-018-0630-3 [ DOI] [ PubMed] 5. De Kievit TR, Gillis R, Marx S, Brown C, Iglewski BH. Quorum-sensing genes in Pseudomonas aeruginosa biofilms: their role and expression patterns. Appl Environ Microbiol. 2001 Apr; 67(4): 1865-73. DOI: 10.1128/AEM.67.4.1865-1873.2001 [ DOI] [ PubMed] 6. Drenkard E. Antimicrobial resistance of Pseudomonas aeruginosa biofilms. Microbes Infect. 2003 Nov; 5(13): 1213-19. DOI: 10.1016/j.micinf.2003.08.009 [ DOI] [ PubMed] 7. Chaudhry WN, Concepción-Acevedo J, Park T, Andleeb S, Bull JJ, Levin BR. Synergy and Order Effects of Antibiotics and Phages in Killing Pseudomonas aeruginosa Biofilms. PLoS One. 2017 Jan; 12(1): e0168615. DOI: 10.1371/journal.pone.0168615 [ DOI] [ PubMed] 8. Mohebi S, Hossieni Nave H, Norouzi A, Kandehkar Gharaman M, Taati Moghadam M. [Detection of Extended Spectrum Beta Lactamases on Class I Integron in Escherichia coli Isolated from Clinical Samples]. J Mazandaran Univ Med Sci. 2016; 26(138): 66-76. [Article in Persian] [ View at Publisher] 9. Ebrahimpour M, Nikokar I, Ghasemi Y, Sedigh Ebrahim-Saraie H, Araghian A, Farahbakhsh M, et al. [Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa isolates obtained from wastewaters of a burn center in Northern Iran]. Ann Ig. 2018 Mar-Apr; 30(2): 112-19. DOI: 10.7416/ai.2018.2202 [ DOI] [ PubMed] 10. Shariati A, Asadian E, Fallah F, Azimi T, Hashemi A, Yasbolaghi Sharahi J, et al. Evaluation of Nano-curcumin effects on expression levels of virulence genes and biofilm production of multidrug-resistant Pseudomonas aeruginosa isolated from burn wound infection in Tehran, Iran. Infect Drug Resist. 2019 Jul; 12: 2223-35. DOI: 10.2147/IDR.S213200 [ DOI] [ PubMed] 11. Sadeghi Dosari A, Norouzi A, Taati Moghadam M, Satarzadeh N. [Antimicrobial Activity Of Ephedra Pachyclada Methanol Extract On Some Enteric Gram Negative Bacteria Which Causes Nosocomial Infections By Agar Dilution Method]. Zahedan J Res Med Sci. 2016 Nov; 18(11): e4015. DOI: 10.17795/zjrms-4015 [ View at Publisher] [ DOI] 12. Caselli A, Cirri P, Santi A, Paoli P. Morin: A Promising Natural Drug. Curr Med Chem. 2016; 23(8): 774-91. DOI: 10.2174/0929867323666160106150821 [ DOI] [ PubMed] 13. Sivaranjani M, Gowrishankar S, Kamaladevi A, Pandian SK, Balamurugan K, Ravi AV. Morin inhibits biofilm production and reduces the virulence of Listeria monocytogenes - An in vitro and in vivo approach. Int J Food Microbiol. 2016 Nov; 237: 73-82. DOI: 10.1016/j.ijfoodmicro.2016.08.021 [ DOI] [ PubMed] 14. Hadizadeh M, Norouzi A, Taghadosi R, Mohebi S, Mohammadi M, Hasanzadeh A, et al. Prevalence of qnr, intI, and intII genes in extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolated from clinical samples in Iran. Trop J Pharm Res 2017; 16(1): 141-47. DOI: 10.4314/tjpr.v16i1.18 [ View at Publisher] [ DOI] 15. Moradi M, Norouzi A, Taati moghadam M. [Prevalence of bla-CTX-M, bla-SHV, and bla-TEM Genes and Comparison of Antibiotic Resistance Pattern in Extended-spectrum β-lactamase producing and non-producing groups of Klebsiella pneumoniae Isolated from Clinical Samples in Kerman Hospitals]. J Advanced Biomed Sci. 2016; 6(1): 120-28. [Article in Persian] [ View at Publisher] 16. Hodaei MS, Dakhili M, Khalifeh Gholli M. [Identification and Pattern of Antibiotic Bacterial Sensitivity and Resistance in Patients in the Nekoi Hospital of Gom City in 1395]. Applied Biology. 2020; 9(35): 1-10. [Article in Persian] [ View at Publisher] 17. Nasser S, Mabrouk A, Maher A. Colonization of burn wounds in Ain Shams University Burn Unit. Burns. 2003 May; 29(3): 229-33. DOI: 10.1016/s0305-4179(02)00285-1 [ DOI] [ PubMed] 18. Sabharwal N, Dhall S, Chhibber S, Harjai K. Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections. Int J Mol Epidemiol Genet. 2014 Oct; 5(3): 125-34. [ PubMed] 19. Yang N, Ding S, Chen F, Zhang X, Xia Y, Di H, et al. The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa. Mol Microbiol. 2015 May; 96(3): 526-47. DOI: 10.1111/mmi.12954 [ DOI] [ PubMed] 20. Woźnicka E, Kuźniar A, Nowak D, Nykiel E, Kopacz M, Gruszecka J, et al. Comparative study on the antibacterial activity of some flavonoids and their sulfonic derivatives. Acta Pol Pharm. 2013 May-Jun; 70(3): 567-71. [ PubMed] 21. Woźnicka E, Zapała L, Pieniążek E, Kosińska-Pezda M, Ciszkowicz E, Lecka-Szlachta K, et al. Synthesis, characterization and antibacterial studies of Tm(III), Yb(III) and Lu(III) complexes of morin. Journal of Coordination Chemistry. 2017; 70(8): 1451-63. DOI: 10.1080/00958972.2017.1291935 [ Article] [ DOI] 22. Amin MU, Khurram M, Khattak B, Khan J. Antibiotic additive and synergistic action of rutin, morin and quercetin against methicillin resistant Staphylococcus aureus. BMC Complement Altern Med. 2015 Mar; 15: 59. DOI: 10.1186/s12906-015-0580-0 [ DOI] [ PubMed] 23. Aghamollaei H, Azizi Barjini K, Moosazadeh Mogaddam M. [Rapid Detection of Pseudomonas Aeruginosa by PCR Method Using Specific Primers of Quorum Sensing LasI gene]. Armaghane-danesh. 2013; 18(9): 722-35. [Article in Persian] [ View at Publisher] 24. Chemmugil P, Lakshmi PTV, Annamalai A. Exploring Morin as an anti-quorum sensing agent (anti-QSA) against resistant strains of Staphylococcus aureus. Microb Pathog. 2019 Feb; 127: 304-15. DOI: 10.1016/j.micpath.2018.12.007 [ DOI] [ PubMed] |
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