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:: Volume 23, Issue 4 (Winter 2022) ::
J Gorgan Univ Med Sci 2022, 23(4): 58-63 Back to browse issues page
Effect of Morin on Expression of Biofilm Gene of Pseudomonas aeruginosa Isolated from Burn Wounds
Samin Babazadeh1 , Kumarss Amini * 2, Mahsa Kavousi3
1- M.Sc in Microbiology, Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Associate Professor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran. , dr_kumarss_amini@yahoo.com
3- Assistant Professor, Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
Abstract:   (216 Views)
Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen that is a major cause of mortality in immunocompromised patients. One of the most important mechanisms of resistance of this bacterium is biofilm formation. The aim of this study was done to determine the Effect of Morin on Expression of Biofilm Gene of Pseudomonas aeruginosa Isolated from burn wounds by Real time PCR.
Methods: In this descriptive-analytic study 60 sample were collected from burn wounds of patients admitted to the hospitals in Tehran, Iran. Samples were identified by using biochemical methods. The DNA of the isolates was extracted and then antimicrobial activity of morin analyzed by microbroth dilution assay. The presence of biofilm production genes was investigated by PCR. Finally, the expression of lasI gene in combination with Sub-MIC concentration of morin in biofilm-producing bacteria was evaluated using Real time PCR.
Results: From 60 samples that analyzed by Multiplex-PCR, 12 (20%) Pseudomonas aeruginosa strains were isolated in which 12 isolates (100%) were carried lasI and lasR, genes, respectively. 3 isolates (25%) were carried rhlI gene. Sub-MIC concentration of morin in biofilm-producing bacteria reduced lasI gene expression in Pseudomonas aeruginosa.
Conclusion: Morin has significant efficacy on Pseudomonas aeruginosa and could be a good alternative for treatment of antibiotic resistant isolates.
Keywords: Pseudomonas aeruginosa [MeSH], Biofilms [MeSH], Morin [MeSH], Real time PCR [MeSH], Drug Resistance [MeSH],
Article ID: Vol23-55
Full-Text [PDF 467 kb]   (795 Downloads)    
Type of Study: Original Articles | Subject: Microbiology
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Babazadeh S, Amini K, Kavousi M. Effect of Morin on Expression of Biofilm Gene of Pseudomonas aeruginosa Isolated from Burn Wounds. J Gorgan Univ Med Sci. 2022; 23 (4) :58-63
URL: http://goums.ac.ir/journal/article-1-3906-en.html


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Volume 23, Issue 4 (Winter 2022) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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