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:: Volume 18, Issue 3 (10-2016) ::
J Gorgan Univ Med Sci 2016, 18(3): 111-117 Back to browse issues page
Genomic structure of Burkholderia mallei Razi 325, the strain used for industrial production of Mallein in Iran
E Faraj Tabrizi1 , K Tadayon * 2, N Mosavari3 , Tajbakhsh E4 , Keshavarz R5 , Ghaderi R5 , Sekhavati M6 , Banihashemi R6 , Najafpour R7 , Mohrekesh Haghighat M8 , Dehghanpour M9
1- M.Sc in Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. M.Sc in Microbiology, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2- Associate Professor, Department of Microbiology, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. , k.tadayon@rvsri.ac.ir
3- Associate Professor, Department of Microbiology, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
4- Associate Professor, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
5- Veterinarian, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
6- M.Sc in Vaccine and Serum, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
7- M.Sc in Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
8- M.Sc in Microbiology, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
9- B.Sc in Laboratory Sciences, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Abstract:   (11303 Views)

Background and Objective: Iran remains a major stronghold for glanders in the Middle East. In Iran, the non-indigenous Burkholderia mallei Razi 325 strain is used in manufacturing of the mallein, required for malleination of animals. Multi Locus Variable number tandem repeat analysis is currently the standard globally accepted genotyping system for Burkholderia mallei. This study was done to survey the genomic structure of Burkholderia mallei Razi 325, the strain used for industrial production of Mallein.

Methods: In this descriptive study, a MLVA genotyping system with 4 previously-characterized loci VNTR140, VNTR1367, VNTR2065, VNTR2971 along with two new loci of VNTR24, VNTR41 was used.

Results: Optimization of PCRs resulted in a single protocol that enabled simultaneous amplification of all the six loci. Sequencing of PCR products revealed there were 2, 3, 12, 6, 1 and 2 copies of the unit repeat hold in the genome of the Burkholderia mallei Razi 325 strain. This observation was extended to include the already-whole genome sequenced Chinese Burkholderia mallei ATCC 23344 and Burkholderia mallei BMQ and also Burkholderia mallei SAVP1 strains.

Conclusion: The Burkholderia mallei Razi 325 strain is distinguishable from the other three strains through MLVA genotyping method.
Keywords: Glanders, Mallein, Burkholderia mallei Razi 325, MLVA genotyping, VNTR
Full-Text [PDF 255 kb] [English Abstract]   (16708 Downloads) |   |   Abstract (HTML)  (1800 Views)  
Type of Study: Original Articles | Subject: Microbiology
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Faraj Tabrizi E, Tadayon K, Mosavari N, Tajbakhsh E, Keshavarz R, Ghaderi R, et al . Genomic structure of Burkholderia mallei Razi 325, the strain used for industrial production of Mallein in Iran. J Gorgan Univ Med Sci 2016; 18 (3) :111-117
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Volume 18, Issue 3 (10-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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