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Determining the Protective Effect of L-Arginine Against Amikacin-Induced Nephrotoxicity in Normal African Green Monkey Kidney Epithelial Cells by Evaluating Oxidative Stress Parameters
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Elahe Gharehkhani1    , Marzieh Megharad2 , Mahboube Rahmati Kukandeh1 , Mohammad Shokrzadeh *3 |
1- Ph.D in Toxicology, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
2- Pharmacy Student, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Ramsar, Iran.
3- Professor, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran. Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran. , mshokrzadeh@mazums.ac.ir |
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Abstract: (622 Views) |
Background and Objective: Due to high metabolic activity and rich blood supply, the kidneys are exposed to high levels of reactive oxygen species (ROS) under pathological conditions, making them highly vulnerable to oxidative stress. Nephrotoxic agents, such as cisplatin, aminoglycosides, and radiocontrast agents induce the production of ROS in renal tubular cells, leading to lipid peroxidation, protein oxidation, and mitochondrial dysfunction. This study was conducted to determine the protective effect of L-arginine against amikacin-induced nephrotoxicity in normal African green monkey kidney epithelial cells (Vero) by evaluating oxidative stress parameters.
Methods: This descriptive-analytical in vitro study was conducted on Vero cell lines purchased from the National Genetic Resources Cell Bank. For all assays, the amount of cultured calls was 105. Prior to the induction of nephrotoxicity with amikacin (653.2 µg/mL), the cells were pre-treated for 24 hours with various concentrations of L-arginine (108, 216, 430, and 860 µM). Subsequently, to evaluate the effect of L-arginine on oxidative stress status, the variables of malondialdehyde (MDA), cell viability, and ROS were measured.
Results: In the assays for ROS levels and cell viability, all tested concentrations of L-arginine (108, 216, 430, and 860 µM) resulted in a significant reduction in ROS levels (30±1.5, 28±1.4, 25±1.2, and 21±1.0, respectively) and a significant increase in cell viability (55±5.2, 64±3.8, 72±2.9, and 84±4.7, respectively) (P<0.05). Regarding measurement tests of lipid peroxidation, L-arginine at 108 µM did not significantly reduce MDA levels; however, other concentrations (216, 430, and 860 µM) significantly decreased MDA levels to 0.80±0.02, 0.74±0.03, and 0.66±0.01, respectively (P<0.05).
Conclusion: The findings of this study demonstrate the ability of L-arginine to improve kidney cell viability parameters and increase glutathione (GSH) levels at all tested concentrations (108, 216, 430, and 860 µM). Furthermore, L-arginine at concentrations of 216, 430, and 860 µM significantly reduced lipid peroxidation.
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Keywords: Oxidative Stress [MeSH], Vero Cells [MeSH], Amikacin [MeSH], Arginine [MeSH]
Article ID: Vol27-37 |
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Full-Text [PDF 1032 kb]
(126 Downloads)
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Type of Study: Original Articles |
Subject:
Pharmacology
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Gharehkhani E, Megharad M, Rahmati Kukandeh M, Shokrzadeh M. Determining the Protective Effect of L-Arginine Against Amikacin-Induced Nephrotoxicity in Normal African Green Monkey Kidney Epithelial Cells by Evaluating Oxidative Stress Parameters. J Gorgan Univ Med Sci 2025; 27 (4) :63-72 URL: http://goums.ac.ir/journal/article-1-4540-en.html
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