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Effect of Concurrent Training with Different Order, Resistance and Endurance Training on Sphingosine-1-Phosphate in Slow and Fast Twitch Muscles in Wistar Rats
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Maliheh Bagheri1    , Amin Farzaneh Hesari * 2, Hajar Abaszadeh3 |
1- M.Sc in Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
2- Assistant Professor, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran. , af.hessari@gmail.com
3- Associate Professor, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran. |
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Abstract: (4583 Views) |
Background and Objective: Sphingosine-1-phosphate (S1P) plays an important role in muscle biology and is involved in hypertrophy and activation of satellite cells. This study was done to determine the effect of eight weeks of concurrent training with different order on S1P in fast and slow muscles in wistar rats.
Methods: In this experimental study, 40 male wistar rats (weight 180-200 gr) were randomly allocated into 5 groups including control, resistance training, endurance training, resistance- endurance training, endurance- resistance training. Resistance training consisted 1-meter ladder climbing with the loading of percent of overloading test (%75 of weight body) in the first week and increased to 30 gr per week. Endurance training consisted of running on treadmill with speed of 12 m/min and 10 minutes in the first week to 30 m/min and 60 minutes in the last week. The resistance- endurance group performed resistance training 5 minutes before endurance training and the endurance- resistance group performed endurance training 5 minutes after resistance training. The protein level of S1P was measured in flexor hallucis longus (FHL) and soleus muscles.
Results: The S1P level in fast and slow twitch muscle increased significantly in endurance- resistance training, resistance- endurance training and resistance training compared to controls (P<0.05). There were no significant differences between concurrent training with differing order.
Conclusion: It seems that exercise order in concurrent training result in no different effect on S1P level in fast and slow twitch muscles in wistar rats. |
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Keywords: Resistance Training [MeSH], Endurance Training [MeSH], Skeletal Muscle [MeSH], Sphingolipids [MeSH], Slow Twitch Muscle [MeSH], Fast Twitch Muscle [MeSH]
Article ID: Vol24-05 |
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Full-Text [PDF 801 kb]
(12190 Downloads)
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Type of Study: Original Articles |
Subject:
Exercise Physiology
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1. Jones TW, Walshe IH, Hamilton DL, Howatson G, Russell M, Price OJ, et al. Signaling Responses After Varying Sequencing of Strength and Endurance Training in a Fed State. Int J Sports Physiol Perform. 2016 Oct; 11(7): 868-75. DOI: 10.1123/ijspp.2015-0534 [ DOI] [ PubMed] 2. Fyfe JJ, Bishop DJ, Stepto NK. Interference between concurrent resistance and endurance exercise: molecular bases and the role of individual training variables. Sports Med. 2014 Jun; 44(6): 743-62. DOI: 10.1007/s40279-014-0162-1 [ DOI] [ PubMed] 3. Schiaffino S, Dyar KA, Ciciliot S, Blaauw B, Sandri M. Mechanisms regulating skeletal muscle growth and atrophy. FEBS J. 2013 Sep; 280(17): 4294-314. DOI: 10.1111/febs.12253 [ DOI] [ PubMed] 4. Zeidan YH, Hannun YA. Translational aspects of sphingolipid metabolism. Trends Mol Med. 2007 Aug; 13(8): 327-36. DOI: 10.1016/j.molmed.2007.06.002 [ DOI] [ PubMed] 5. Germinario E, Peron S, Toniolo L, Betto R, Cencetti F, Donati C, et al. S1P2 receptor promotes mouse skeletal muscle regeneration. J Appl Physiol (1985). 2012 Sep; 113(5): 707-13. DOI: 10.1152/japplphysiol.00300.2012 [ DOI] [ PubMed] 6. Squecco R, Sassoli C, Nuti F, Martinesi M, Chellini F, Nosi D, et al. Sphingosine 1-phosphate induces myoblast differentiation through Cx43 protein expression: a role for a gap junction-dependent and -independent function. Mol Biol Cell. 2006 Nov; 17(11): 4896-910. DOI: 10.1091/mbc.e06-03-0243 [ DOI] [ PubMed] 7. Ogasawara R, Kobayashi K, Tsutaki A, Lee K, Abe T, Fujita S, et al. mTOR signaling response to resistance exercise is altered by chronic resistance training and detraining in skeletal muscle. J Appl Physiol (1985). 2013 Apr; 114(7): 934-40. DOI: 10.1152/japplphysiol.01161.2012 [ DOI] [ PubMed] 8. Meacci E, Bini F, Sassoli C, Martinesi M, Squecco R, Chellini F, et al. Functional interaction between TRPC1 channel and connexin-43 protein: a novel pathway underlying S1P action on skeletal myogenesis. Cell Mol Life Sci. 2010 Dec; 67(24): 4269-85. DOI: 10.1007/s00018-010-0442-3 [ DOI] [ PubMed] 9. Eddens L, van Someren K, Howatson G. The Role of Intra-Session Exercise Sequence in the Interference Effect: A Systematic Review with Meta-Analysis. Sports Med. 2018 Jan; 48(1): 177-88. DOI: 10.1007/s40279-017-0784-1 [ DOI] [ PubMed] 10. Eklund D, Schumann M, Kraemer WJ, Izquierdo M, Taipale RS, Häkkinen K. Acute Endocrine and Force Responses and Long-Term Adaptations to Same-Session Combined Strength and Endurance Training in Women. J Strength Cond Res. 2016 Jan; 30(1): 164-75. DOI: 10.1519/JSC.0000000000001022 [ DOI] [ PubMed] 11. Apró W, Moberg M, Hamilton DL, Ekblom B, van Hall G, Holmberg HC, et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab. 2015 Mar; 308(6): E470-81. DOI: 10.1152/ajpendo.00486.2014 [ DOI] [ PubMed] 12. Pugh JK, Faulkner SH, Jackson AP, King JA, Nimmo MA. Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle. Physiol Rep. 2015 Apr; 3(4): e12364. DOI: 10.14814/phy2.12364 [ DOI] [ PubMed] 13. Sangdevini M, Fallah Mohammadi Z, Oladnabi M. [Effect of 8 weeks of resistance training and concurrent resistance-aerobic training on phospho-mTOR and phospho-p70S6K responses in skeletal muscle of rat]. J Gorgan Univ Med Sci. 2020; 22(1): 43-49. [Article in Persian] [ View at Publisher] 14. Banitalebi E, Gharakhanlou R, Ghatrehsamani K, Parnow AH, Teimori H, Mohammad Amoli M. The effect of resistance training on plasma S1P level and gene expression of S1P1,2,3 receptors in male Wistar rats. Minerva Endocrinol. 2013 Dec; 38(4): 395-400 [ PubMed] 15. Błachnio-Zabielska A, Baranowski M, Zabielski P, Górski J. Effect of exercise duration on the key pathways of ceramide metabolism in rat skeletal muscles. J Cell Biochem. 2008 Oct; 105(3): 776-84. DOI: 10.1002/jcb.21877 [ DOI] [ PubMed] 16. Baranowski M, Charmas M, Długołęcka B, Górski J. Exercise increases plasma levels of sphingoid base-1 phosphates in humans. Acta Physiol (Oxf). 2011 Nov; 203(3): 373-80. DOI: 10.1111/j.1748-1716.2011.02322.x [ DOI] [ PubMed] 17. Baranowski M, Błachnio-Zabielska AU, Charmas M, Helge JW, Dela F, Książek M, et al. Exercise increases sphingoid base-1-phosphate levels in human blood and skeletal muscle in a time- and intensity-dependent manner. Eur J Appl Physiol. 2015 May; 115(5): 993-1003. DOI: 10.1007/s00421-014-3080-x [ DOI] [ PubMed] 18. Baranowski M, Górski J, Klapcinska B, Waskiewicz Z, Sadowska-Krepa E. Ultramarathon run markedly reduces plasma sphingosine-1-phosphate concentration. Int J Sport Nutr Exerc Metab. 2014 Apr; 24(2): 148-56. DOI: 10.1123/ijsnem.2013-0093 [ DOI] [ PubMed] 19. Hannun YA, Obeid LM. Principles of bioactive lipid signalling: lessons from sphingolipids. Nat Rev Mol Cell Biol. 2008 Feb; 9(2): 139-50. DOI: 10.1038/nrm2329 [ DOI] [ PubMed] 20. Kazior Z, Willis SJ, Moberg M, Apró W, Calbet JA, Holmberg HC, et al. Endurance Exercise Enhances the Effect of Strength Training on Muscle Fiber Size and Protein Expression of Akt and mTOR. PLoS One. 2016 Feb; 11(2): e0149082. DOI: 10.1371/journal.pone.0149082 [ DOI] [ PubMed] 21. Rahbek SK, Farup J, Møller AB, Vendelbo MH, Holm L, Jessen N, et al. Effects of divergent resistance exercise contraction mode and dietary supplementation type on anabolic signalling, muscle protein synthesis and muscle hypertrophy. Amino Acids. 2014 Oct; 46(10): 2377-92. DOI: 10.1007/s00726-014-1792-1 [ DOI] [ PubMed] 22. de Souza EO, Tricoli V, Bueno Junior C, Pereira MG, Brum PC, Oliveira EM, et al. The acute effects of strength, endurance and concurrent exercises on the Akt/mTOR/p70(S6K1) and AMPK signaling pathway responses in rat skeletal muscle. Braz J Med Biol Res. 2013 Apr; 46(4): 343-47. DOI: 10.1590/1414-431x20132557 [ DOI] [ PubMed] 23. Shamim B, Camera DM, Whitfield J. Myofibre Hypertrophy in the Absence of Changes to Satellite Cell Content Following Concurrent Exercise Training in Young Healthy Men. Front Physiol. 2021 Jun; 12: 625044. DOI: 10.3389/fphys.2021.625044 [ DOI] [ PubMed] 24. Murlasits Z, Kneffel Z, Thalib L. The physiological effects of concurrent strength and endurance training sequence: A systematic review and meta-analysis. J Sports Sci. 2018 Jun; 36(11): 1212-19. DOI: 10.1080/02640414.2017.1364405 [ DOI] [ PubMed] 25. Shirai T, Aoki Y, Takeda K, Takemasa T. The order of concurrent training affects mTOR signaling but not mitochondrial biogenesis in mouse skeletal muscle. Physiol Rep. 2020 Apr; 8(7): e14411. DOI: 10.14814/phy2.14411 [ DOI] [ PubMed] 26. Sousa AC, Neiva HP, Izquierdo M, Alves AR, Duarte-Mendes P, Ramalho AG, et al. Concurrent Training Intensities: A Practical Approach for Program Design. Strength Cond J. 2020 Apr; 42(2): 38-44. DOI: 10.1519/SSC.0000000000000520 [ View at Publisher] [ DOI] 27. Coffey VG, Jemiolo B, Edge J, Garnham AP, Trappe SW, Hawley JA. Effect of consecutive repeated sprint and resistance exercise bouts on acute adaptive responses in human skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2009 Nov; 297(5): R1441-51. DOI: 10.1152/ajpregu.00351.2009 [ DOI] [ PubMed] 28. Hawley JA. Molecular responses to strength and endurance training: are they incompatible? Appl Physiol Nutr Metab. 2009 Jun; 34(3): 355-61. DOI: 10.1139/H09-023 [ DOI] [ PubMed] 29. Shirai T, Hanakita H, Uemichi K, Takemasa T. Effect of the order of concurrent training combined with resistance and high-intensity interval exercise on mTOR signaling and glycolytic metabolism in mouse skeletal muscle. Physiol Rep. 2021 Mar; 9(5): e14770. DOI: 10.14814/phy2.14770 [ DOI] [ PubMed] 30. Lundberg TR, Fernandez-Gonzalo R, Gustafsson T, Tesch PA. Aerobic exercise alters skeletal muscle molecular responses to resistance exercise. Med Sci Sports Exerc. 2012 Sep; 44(9): 1680-88. DOI: 10.1249/MSS.0b013e318256fbe8 [ DOI] [ PubMed] |
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Bagheri M, Hesari A F, Abaszadeh H. Effect of Concurrent Training with Different Order, Resistance and Endurance Training on Sphingosine-1-Phosphate in Slow and Fast Twitch Muscles in Wistar Rats. J Gorgan Univ Med Sci 2022; 24 (1) :35-43 URL: http://goums.ac.ir/journal/article-1-3917-en.html
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