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Protective Effect of Aerobic Exercise and Adenosine on Changes in Inflammation Mediators after Transient Ischemia of Common Carotid Arteries in Male Wistar Rats
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Zahra Eslami1    , Zeinab Mohammadi2 , Shohreh Sharifian3 , Masoumeh Rezaei Ghomi4 , Seyedeh Vafa Mousavi4 , Mahboubeh Farhadi4 , Najmeh Sheikh Robati4 , Zeinab Faghfoori5 , Seyed Javad Mirghani *6 |
1- Ph.D Candidate in Clinical Biochemistry, Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Ph.D Candidate in Clinical Biochemistry, Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
3- Ph.D in Exercise Physiology, Central Organization of Islamic Azad University, Tehran, Iran.
4- M.Sc in Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
5- Ph.D in Nutrition, Department of Nutrition, Aradan School of Nutrition and Food Sciences, Semnan University of Medical Sciences, Aradan, Iran.
6- Ph.D in Exercise Physiology, Shahid Mirghani Research Institute, Golestan, Iran. , seyedgavadmirghani@yahoo.com |
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Abstract: (9559 Views) |
Background and Objective: Stroke and cerebral ischemia are the second leading causes of death in the world. Currently, there are limited therapeutic interventions for patients with ischemia / reperfusion. This study was performed to determine the protective effect of aerobic exercise and adenosine on changes in inflammation mediators after transient ischemia of common carotid arteries in male Wistar rats.
Methods: In this experimental study, fifty male Wistar rats were randomly allocated into to 5 groups: control, cerebral ischemic control, aerobic exercise + cerebral ischemia, adenosine + cerebral ischemia and aerobic exercise + adenosine + cerebral ischemia. Ischemia was performed by blocking the common carotid artery for 45 minutes after a period of exercise and injection of adenosine. Neuronal structure was examined by Nissel tissue staining. The expression of NGF and Glutamate genes were measured in CA1 region of hippocampal tissue samples.
Results: Cell death was increased in neurons in the CA1 region of the hippocampus in the ischemia / reperfusion group, While a significant reduction in cell death in the adenosine + ischemia / reperfusion and aerobic exercise + ischemia /reperfusion groups was due to adenosine administration and aerobic exercise (P<0.05). NGF and glutamate gene expression in the adenosine + ischemia/reperfusion and adenosine + aerobic exersice + ischemia/reperfusion groups significantly increased and reduced compared to the ischemia/reperfusion control, respectively (P<0.05).
Conclusion: Co-administration of adenosine combined with aerobic exercise increase the protective effect of aerobic exercise on improving the neuronal damage after ischemic / reperfusion. |
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Keywords: Ischemia [MeSH], Aerobic Exercise [MeSH], Adenosine [MeSH], Inflammation Mediators [MeSH]
Article ID: Vol23-37 |
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Full-Text [PDF 841 kb]
(20139 Downloads)
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Type of Study: Original Articles |
Subject:
Exercise Physiology
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1. Campbell BCV, De Silva DA, Macleod MR, Coutts SB, Schwamm LH, Davis SM, et al. Ischaemic stroke. Nat Rev Dis Primers. 2019 Oct; 5(1): 70. DOI: 10.1038/s41572-019-0118-8 [ DOI] [ PubMed] 2. Zhang F, Jia J, Wu Y, Hu Y, Wang Y. The effect of treadmill training pre-exercise on glutamate receptor expression in rats after cerebral ischemia. Int J Mol Sci. 2010 Jul; 11(7): 2658-69. DOI: 10.3390/ijms11072658 [ DOI] [ PubMed] 3. Ganesana M, Venton BJ. Early changes in transient adenosine during cerebral ischemia and reperfusion injury. PLoS One. 2018 May; 13(5): e0196932. DOI: 10.1371/journal.pone.0196932 [ DOI] [ PubMed] 4. Gomes C, Ferreira R, George J, Sanches R, Rodrigues DI, Gonçalves N, et al. Activation of microglial cells triggers a release of brain-derived neurotrophic factor (BDNF) inducing their proliferation in an adenosine A2A receptor-dependent manner: A2A receptor blockade prevents BDNF release and proliferation of microglia. J Neuroinflammation. 2013 Jan; 10: 16. DOI: 10.1186/1742-2094-10-16 [ DOI] [ PubMed] 5. Keefe KM, Sheikh IS, Smith GM. Targeting Neurotrophins to Specific Populations of Neurons: NGF, BDNF, and NT-3 and Their Relevance for Treatment of Spinal Cord Injury. Int J Mol Sci. 2017 Mar; 18(3): 548. DOI: 10.3390/ijms18030548 [ DOI] [ PubMed] 6. Gonçalves Mourão FA, Leite HR, de Carvalho LED, Vieira TH FE, Pinto MCX, de Castro Medeiros D, et al. Neuroprotective effect of exercise in rat hippocampal slices submitted to in vitro ischemia is promoted by decrease of glutamate release and pro-apoptotic markers. J Neurochem. 2014 Oct; 131(1): 65-73. DOI: 10.1111/jnc.12786 [ DOI] [ PubMed] 7. Cobianchi S, Arbat-Plana A, Lopez-Alvarez VM, Navarro X. Neuroprotective Effects of Exercise Treatments After Injury: The Dual Role of Neurotrophic Factors. Curr Neuropharmacol. 2017; 15(4): 495-518. DOI: 10.2174/1570159X14666160330105132 [ DOI] [ PubMed] 8. Aboutaleb N, Shamsaei N, Rajabi H, Khaksari M, Erfani S, Nikbakht F, et al. Protection of Hippocampal CA1 Neurons Against Ischemia/Reperfusion Injury by Exercise Preconditioning via Modulation of Bax/Bcl-2 Ratio and Prevention of Caspase-3 Activation. Basic Clin Neurosci. 2016 Jan; 7(1): 21-9. [ PubMed] 9. Meldrum BS. Glutamate as a neurotransmitter in the brain: review of physiology and pathology. J Nutr. 2000 Apr; 130(4S Suppl): 1007S-15S. DOI: 10.1093/jn/130.4.1007S [ DOI] [ PubMed] 10. Rabiei Z, Bigdeli M, Mohagheghi F, Rasolian B. Relationship between dietary virgin Olive oil on brain Cholesterol, Cholesteryl ester and Triglyceride levels and Blood Brain Barrier (BBB) permeability in a rat stroke model. Physiol Pharmacol. 2012; 16(3): 245-54. [ View at Publisher] 11. Erfani S, Khaksari M, Oryan S, Shamsaei N, Aboutaleb N, Nikbakht F. Nampt/PBEF/visfatin exerts neuroprotective effects against ischemia/reperfusion injury via modulation of Bax/Bcl-2 ratio and prevention of caspase-3 activation. J Mol Neurosci. 2015 May; 56(1): 237-43. DOI: 10.1007/s12031-014-0486-1 [ DOI] [ PubMed] 12. Faghani M, Jafari Z, Molladost H, Nadia Sharifi Z. [Effects of verapamil on CA1 pyramidal cells of hippocampus following ischemia reperfusion in rats]. Med Sci J Islam Azad Univ Tehran Med Branch. 2015; 25(1): 33-38. [Article in Persian] [ View at Publisher] 13. Mirghani SJ, Peeri M, Yaghoobpour Yekani O, Zamani M, Feizolahi F, Nikbin S, et al. Role or Synergistic Interaction of Adenosine and Vitamin D3 Alongside High-Intensity Interval Training and Isocaloric Moderate Intensity Training on Metabolic Parameters: Protocol for an Experimental Study. JMIR Res Protoc. 2019 Jan; 8(1): e10753. DOI: 10.2196/10753 [ DOI] [ PubMed] 14. Zhao ZQ, Budde JM, Morris C, Wang NP, Velez DA, Muraki S, et al. Adenosine attenuates reperfusion-induced apoptotic cell death by modulating expression of Bcl-2 and Bax proteins. J Mol Cell Cardiol. 2001 Jan; 33(1): 57-68. DOI: 10.1006/jmcc.2000.1275 [ DOI] [ PubMed] 15. Jozaie A, Movahedi M, Khosravi M, Golab F. [The effects of adenosine injection after of brain ischemia reperfusion injury on gene expression of NF-kB/p65 and activity level of ROS in male Wistar rats]. Razi j Med Sci. 2019; 26(2): 74-84. [Article in Persian] [ View at Publisher] 16. Zhang ZH, Xi GM, Li WC, Ling HY, Qu P, Fang XB. Cyclic-AMP response element binding protein and tau are involved in the neuroprotective mechanisms of nerve growth factor during focal cerebral ischemia/reperfusion in rats. J Clin Neurosci. 2010 Mar; 17(3): 353-56. DOI: 10.1016/j.jocn.2009.07.086 [ DOI] [ PubMed] 17. Muroi Y, Ishii T, Teramoto K, Hori M, Nishimura M. Calcineurin contributes to the enhancing effect of adenosine on nerve growth factor-induced neurite outgrowth via the decreased duration of p38 mitogen-activated protein kinase phosphorylation. J Pharmacol Sci. 2004 May; 95(1): 124-31. DOI: 10.1254/jphs.95.124 [ DOI] [ PubMed] 18. Liu YJ, Chen J, Li X, Zhou X, Hu YM, Chu SF, et al. Research progress on adenosine in central nervous system diseases. CNS Neurosci Ther. 2019 Sep; 25(9): 899-910. DOI: 10.1111/cns.13190 [ DOI] [ PubMed] 19. Gao X, Xu X, Pang J, Zhang C, Ding JM, Peng X, et al. NMDA receptor activation induces mitochondrial dysfunction, oxidative stress and apoptosis in cultured neonatal rat cardiomyocytes. Physiol Res. 2007; 56(5): 559-69. DOI: 10.33549/physiolres.931053 [ DOI] [ PubMed] 20. Feng R, Zhang M, Wang X, Li WB, Ren SQ, Zhang F. Pre-ischemic exercise alleviates oxidative damage following ischemic stroke in rats. Exp Ther Med. 2014 Oct; 8(4): 1325-29. DOI: 10.3892/etm.2014.1874 [ DOI] [ PubMed] 21. Zhang F, Wu Y, Jia J. Exercise preconditioning and brain ischemic tolerance. Neuroscience. 2011 Mar; 177: 170-76. DOI: 10.1016/j.neuroscience.2011.01.018 [ DOI] [ PubMed] 22. Jia J, Hu YS, Wu Y, Yu HX, Liu G, Zhu DN, et al. Treadmill pre-training suppresses the release of glutamate resulting from cerebral ischemia in rats. Exp Brain Res. 2010 Jul; 204(2): 173-79. DOI: 10.1007/s00221-010-2320-5 [ DOI] [ PubMed] 23. Rashedul Islam M, Young MF, Wrann CD. Neuroprotective potential of exercise preconditioning in stroke. Cond Med. 2017; 1(1): 27-34. [ PubMed] 24. Ding YH, Luan XD, Li J, Rafols JA, Guthinkonda M, Diaz FG, Ding Y. Exercise-induced overexpression of angiogenic factors and reduction of ischemia/reperfusion injury in stroke. Curr Neurovasc Res. 2004 Dec; 1(5): 411-20. DOI: 10.2174/1567202043361875 [ DOI] [ PubMed] 25. Ding Y, Li J, Luan X, Ding YH, Lai Q, Rafols JA, et al. Exercise pre-conditioning reduces brain damage in ischemic rats that may be associated with regional angiogenesis and cellular overexpression of neurotrophin. Neuroscience. 2004; 124(3): 583-91. DOI: 10.1016/j.neuroscience.2003.12.029 [ DOI] [ PubMed] 26. Mizutani K, Sonoda S, Yamada K, Beppu H, Shimpo K. Alteration of protein expression profile following voluntary exercise in the perilesional cortex of rats with focal cerebral infarction. Brain Res. 2011 Oct; 1416: 61-68. DOI: 10.1016/j.brainres.2011.08.012 [ DOI] [ PubMed] 27. amadi A. [Exercise Preconditioning and Neuroprotection: A Review of Mechanisms]. Shefaye Khatam. 2015; 3(1): 115-30. DOI: 10.18869/acadpub.shefa.3.1.115 [Article in Persian] [ View at Publisher] [ DOI] 28. Zhang Y, Cao RY, Jia X, Li Q, Qiao L, Yan G, et al. Treadmill exercise promotes neuroprotection against cerebral ischemia-reperfusion injury via downregulation of pro-inflammatory mediators. Neuropsychiatr Dis Treat. 2016 Dec; 12: 3161-73. DOI: 10.2147/NDT.S121779 [ DOI] [ PubMed] 29. Terashi T, Otsuka S, Takada S, Nakanishi K, Ueda K, Sumizono M. Neuroprotective effects of different frequency preconditioning exercise on neuronal apoptosis after focal brain ischemia in rats. Neurol Res. 2019 Jun; 41(6): 510-18. DOI: 10.1080/01616412.2019.1580458 [ DOI] [ PubMed] |
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Eslami Z, Mohammadi Z, Sharifian S, Rezaei Ghomi M, Mousavi S V, Farhadi M, et al . Protective Effect of Aerobic Exercise and Adenosine on Changes in Inflammation Mediators after Transient Ischemia of Common Carotid Arteries in Male Wistar Rats. J Gorgan Univ Med Sci 2021; 23 (3) :32-39 URL: http://goums.ac.ir/journal/article-1-3866-en.html
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