[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aims and Scope
Content Coverage
Conflict of Interest
Publication Ethics
Editorial Board::
Executive Members::
Instruction to Authors::
Peer Review::
Articles Archive::
Current Issue
All Issues
Indexing Databases::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 20, Issue 1 (3-2018) ::
J Gorgan Univ Med Sci 2018, 20(1): 58-63 Back to browse issues page
Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice
Nastaran Amintaheri1 , Maryam Tehranipour * 2, Saeedeh Zafar Balanezhad3
1- MSc in Animal Biology, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2- Associate Professor, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. maryam_tehranipour@mshdiau.ac.ir , maryam_tehranipour@mshdiau.ac.ir
3- Assistant Professor, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Abstract:   (18771 Views)
Background and Objective: Brain is not able to produce new neurons by neurogenesis after maturity. Neurogenesis after the maturity was reported in Hippocampus and subventricular areas in the brain. Rosa canina L has various vitamins and other valuable compounds such as polyphenols, carotenoid, carbohydrates and fatty acids. This study was conducted to evaluate the effect of the alcoholic extract of the fruit of Rosa canina L plant on neuronal density of the hippocampus in animal model.
Methods: In this experimental study 24 adult male mice were randomly allocated into 4 groups including: control and three treatent groups. Animals in treatment groups 1, 2 and 3 were received the alcoholic extract with extract with a dose of 25, 50, 75 mg/kg/bw intraperitoneally (IP), for 21 day continuously with an invertal of 24 hours, respectively. Animals in control group were received normal saline injection. One month after the first injection, the animals were anesthetized and brain gently was out of the skull. After processing, seven-micron serial sections were stained with blue toluidine and erythrosine. Different regions of the hippocampus were photographed and neuronal density was evaluated by stereological methods and was compared with control group.
Results: The mean neuronal density of CA1 area of hippocampus in control and the treated group with a dose of 25, 50, 75 mg/kg/bw was 55±2, 70±3, 65±3 and 61±2, respectively. Neuronal density significantly increased in treatment group with dosage of 25 mg/kg/bw in compared to control group (P<0.05). The mean neuronal density of CA2 and CA3 area of hippocampus in treated group with a dose of 25, 50, 75 mg/kg/bw was not significant in compared to controls.
Conclusion: This study showed that the alcoholic extract of the fruit of Rosa canina L plant with dosage of 25 mg/kg/bw increase neurons of the mice hippocampus.
Keywords: Hippocampus, Neuronal density, Rosa canina L, Alcoholic extract
Full-Text [PDF 370 kb] [English Abstract]   (16467 Downloads) |   |   Abstract (HTML)  (952 Views)  
Type of Study: Original Articles | Subject: Physiology - Pharmacology
* Corresponding Author Address: Associate Professor, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. maryam_tehranipour@mshdiau.ac.ir
References
1. Atwood CS, Vadakkadath Meethal S. Ganadotropins and Progestogens: Obligatory developmental function during early embryogenesis and their role in adult neurogenesis, neuroregeneration and neurodegeneration. In: Gravanis AG, Mellon SH. Hormones in Neurodegeneration, Neuroprotection, and Neurogenesis. Chap 18. 1st ed. New Jersey: Wiley-Blackwell. 2011; pp:305-29.
2. Gorden MS. Neurobiology. 3rd ed. Oxford: Oxford University Press. 2000; pp: 618-34.
3. Guyton AC, Hall JE. Textbook of Medical physiology. 3rd ed. Philadelphia: Saunders Company. 2008; pp: 643-45.
4. Tehranipour M, Mollashahi M, Javadmoosavi BZ. [Effect of ethanolic extract of pod Prosopis farcta plant on neuronal density of anterior horn following sciatic nerve compression in Rats]. J Gorgan Univ Med Sci. 2012; 14(4): 39-43. [Article in Persian]
5. Tehranipour M, Javadmoosavi BZ, Kehtarpour M, Khayyatzade J. [Effect of aquatic extract of Cannabis sativa leaves on degeneration of alpha motoneurons in spinal cord after sciatic nerve compression in Rats]. J Gorgan Univ Med Sci. 2011; 13(1): 16-22. [Article in Persian]
6. Jalali M, Tehranipour M, Mahdavi Shahri N. [Effect of alcoholic extract of Nigella sativa seed on alpha motor neurons density of spinal cord following sciatic nerve compression in rats]. J Gorgan Univ Med Sci. 2013; 15(4): 29-34. [Article in Persian]
7. Tehranipour M, Lagzian A. [Effect of Hyssopus officinalis leaves alcoholic extract on motor neuron density in anterior horn after sciatic nerve compression in rats]. J Gorgan Univ Med Sci. 2016; 18(4): 36-41. [Article in Persian]
8. Saeedi Aboeshaghi KA, Omidbaigi R. [Study on quantitative and qualitative changes in fatty acids of dog rose (Rosa Canina L.) seeds collected from south-west of Iran]. Journal of Horticulture Science (Agricultural Scienes and Technology). 2009-2010; 23(2): 11-17. [Article in Persian]
9. Emami M, Hosseini AR, Saeedi A, Golbidi D, Reisi P , Alaei H. [Effect of red grape juice on learning and passive avoidance memory in rats]. J Isfahan Med Sch. 2010; 104(28): 1-7. [Article in Persian]
10. Egea I, Sánchez-Bel P, Romojaro F, Pretel MT. Six edible wild fruits as potential antioxidant additives or nutritional supplements. Plant Foods Hum Nutr. 2010 Jun;65(2):121-9.
11. Larsen E, Kharazmi A, Christensen LP, Christensen SB. An antiinflammatory galactolipid from rose hip (Rosa canina) that inhibits chemotaxis of human peripheral blood neutrophils in vitro. J Nat Prod. 2003 Jul; 66(7):994-5. doi:10.1021/np0300636
12. Daels-Rakotoarison DA, Gressier B, Trotin F, Brunet C, Luyckx M, Dine T, et al. Effects of Rosa canina fruit extract on neutrophil respiratory burst. Phytother Res. 2002 Mar; 16(2):157-61.
13. Behnam-Rasouli M, Nikravesh MR, Mahdavi-Shahri N, Tehranipour M. Post-Operative time effects after sciatic nerve crush on the number of alpha motoneurons, using a stereological counting method (disector). Iran Biomed J. 2000 Jan; 4(1): 45-49.
14. Corripio R, Gónzalez-Clemente JM, Jacobo PS, Silvia N, Lluis G, Joan V, et al. Plasma brain-derived neurotrophic factor in prepubertal obese children: results from a 2-year lifestyle intervention programme. Clin Endocrinol (Oxf). 2012 Nov; 77(5):715-20. doi:10.1111/j.1365-2265.2012.04431.x
15. Lommatzsch M, Zingler D, Schuhbaeck K, Schloetcke K, Zingler C, Schuff-Werner P, et al. The impact of age, weight and gender on BDNF levels in human platelets and plasma. Neurobiol Aging. 2005 Jan; 26(1):115-23. doi:10.1016/j.neurobiolaging.2004.03.002
16. Kempermann G, Kuhn HG, Gage FH. More hippocampal neurons in adult mice living in an enriched environment. Nature. 1997 Apr; 386(6624):493-5. doi:10.1038/386493a0
17. Grant MM, Barber VS, Griffiths HR. The presence of ascorbate induces expression of brain derived neurotrophic factor in SH-SY5Y neuroblastoma cells after peroxide insult, which is associated with increased survival. Proteomics. 2005 Feb; 5(2):534-40. doi:10.1002/pmic.200300924
18. Zigova T, Pencea V, Wiegand SJ, Luskin MB. Intraventricular administration of BDNF increases the number of newly generated neurons in the adult olfactory bulb. Mol Cell Neurosci. 1998 Jul; 11(4):234-45. doi:10.1006/mcne.1998.0684
19. Tagliari B, Scherer EB, Machado FR, Ferreira AG, Dalmaz C, Wyse AT. Antioxidants prevent memory deficits provoked by chronic variable stress in rats. Neurochem Res. 2011 Dec; 36(12): 2373-80. doi:10.1007/s11064-011-0563-6
20. Cagle P, Idassi O, Carpenter J, Minor R, Goktepe I, Martin P. Effect of rosehip (Rosa Canina) extracts on human brain tumor cell proliferation and apoptosis. J Cancer Ther. 2012; 3(5): 534-45. doi:10.4236/jct.2012.35069
21. Marchetti C. Molecular targets of lead in brain neurotoxicity. Neurotox Res. 2003;5(3):221-36.
22. Mehla J, Pahuja M, Gupta YK. Streptozotocin-induced sporadic Alzheimer's disease: selection of appropriate dose. J Alzheimers Dis. 2013; 33(1):17-21. doi:10.3233/JAD-2012-120958
23. Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009; 2(5): 270-78. doi:10.4161/oxim.2.5.9498
24. Behnam-Rasouli M, Nikravesh MR, Mahdavi-Shahri N, Tehranipour M. Post-operative time effects after sciatic nerve crush on the number of alpha motoneurons, using a stereological counting method (disector). Iran Biomed J. 2000; 4(1): 45-49.
25. Serteser A, Kargioğlu M, Gök V, Bağci Y, Ozcan MM, Arslan D. Determination of antioxidant effects of some plant species wild growing in Turkey. Int J Food Sci Nutr. 2008 Nov-Dec; 59(7-8): 643-51. doi:10.1080/09637480701602530
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Amintaheri N, Tehranipour M, Zafar Balanezhad S. Effect of alcoholic extracts of Rosa canina L fruit on hippocampus neuronal density in mice . J Gorgan Univ Med Sci 2018; 20 (1) :58-63
URL: http://goums.ac.ir/journal/article-1-3278-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 1 (3-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4660
Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)