Volume 11, Issue 2 (10-2023)
Jorjani Biomed J 2023, 11(2): 19-23 |
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Naderi Noreini S, Ahmadi Nia Tabesh R, Malmir M, Ghafarizade A A. Protective effect of L-carnitine on sperm motility and membrane integrity in men asthenoteratozoospermia: An in vitro study. Jorjani Biomed J 2023; 11 (2) :19-23
URL: http://goums.ac.ir/jorjanijournal/article-1-889-en.html
URL: http://goums.ac.ir/jorjanijournal/article-1-889-en.html
1- Rastak Infertility Center, Arak, Iran
2- Department of Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran
3- Academic Center for Education, Culture and Research, Arak, Iran ,Ghafarizade@acecr.ac.ir
2- Department of Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran
3- Academic Center for Education, Culture and Research, Arak, Iran ,
Abstract: (3126 Views)
Background: Sperm preparation is a time-consuming process that makes a contribution to oxidative stress induction in assisted reproductive techniques.
Objective: This study aimed to evaluate the effect of L-carnitine on sperm parameters during semen preparation at different time intervals.
Materials and Methods: In this in vitro study, the semen samples of 50 asthenoteratozoospermic men were divided into the control and the L-carnitine groups (incubated with 0.5 mg/mL L-carnitine) (n=25/each). Total motility, progressive motility, sperm membrane integrity (by Hypo-osmotic swelling test), and lipid peroxidation (by Malondialdehyde level) were assessed in each group after 2, 4, and 6 hours. Afterward, samples were analyzed by the World Health Organization (2010) criteria.
Result: The results showed that in the control group total and progressive motility, and membrane integrity of the sperm were significantly decreased after 2 hr (P= 0.028, p= 0.019, and p= 0.025, respectively), while Malondialdehyde levels increased significantly after 4 hr (P= 0.018). Sperm parameters in the L-carnitine group increased significantly after 2 hr (P= 0.002), while Malondialdehyde levels decreased significantly compared to the control group in 6 hr (P < 0.001 vs. P =0.045).
Conclusion: Based on these results, L-carnitine can manage to conserve motility and sperm membrane integrity of sperm from the hazardous impact of oxidative stress and lipid peroxidation during sperm preparation.
Objective: This study aimed to evaluate the effect of L-carnitine on sperm parameters during semen preparation at different time intervals.
Materials and Methods: In this in vitro study, the semen samples of 50 asthenoteratozoospermic men were divided into the control and the L-carnitine groups (incubated with 0.5 mg/mL L-carnitine) (n=25/each). Total motility, progressive motility, sperm membrane integrity (by Hypo-osmotic swelling test), and lipid peroxidation (by Malondialdehyde level) were assessed in each group after 2, 4, and 6 hours. Afterward, samples were analyzed by the World Health Organization (2010) criteria.
Result: The results showed that in the control group total and progressive motility, and membrane integrity of the sperm were significantly decreased after 2 hr (P= 0.028, p= 0.019, and p= 0.025, respectively), while Malondialdehyde levels increased significantly after 4 hr (P= 0.018). Sperm parameters in the L-carnitine group increased significantly after 2 hr (P= 0.002), while Malondialdehyde levels decreased significantly compared to the control group in 6 hr (P < 0.001 vs. P =0.045).
Conclusion: Based on these results, L-carnitine can manage to conserve motility and sperm membrane integrity of sperm from the hazardous impact of oxidative stress and lipid peroxidation during sperm preparation.
Type of Article: Original article |
Subject:
Health
Received: 2022/02/5 | Accepted: 2023/09/30 | Published: 2023/12/30
Received: 2022/02/5 | Accepted: 2023/09/30 | Published: 2023/12/30
References
1. Makker K, Agarwal A, Sharma R. Oxidative stress & male infertility. Indian J Med Res. 2009;129(4): 357-67. [View at Publisher] [PMID] [Google Scholar]
2. Fanaei H, Khayat S, Halvaei I, Ramezani V, Azizi Y, Kasaeian A. Effects of ascorbic acid on sperm motility, viability, acrosome reaction and DNA integrity in teratozoospermic samples. Iran J Reprod Med. 2014;12(2):103-10. [View at Publisher] [PMID] [Google Scholar]
3. Lu JC, Huang YF, Lu NQ. [WHO laboratory manual for the examination and processing of human semen. its applicability to andrology laboratories in China]. Zhonghua Nan Ke Xue. 2010;16(10):867-71. [View at Publisher] [PMID] [Google Scholar]
4. Ghafarizadeh AA, Vaezi G, Shariatzadeh MA, Malekirad AA. Effect of in vitro selenium supplementation on sperm quality in asthenoteratozoospermic men. Andrologia. 2018;50(2):e12869. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Safarinejad MR, Hosseini SY, Dadkhah F, Asgari MA. Relationship of omega-3 and omega-6 fatty acids with semen characteristics, and anti-oxidant status of seminal plasma: a comparison between fertile and infertile men. Clin Nutr. 2010;29(1):100-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Nabi A, Khalili MA, Halvaei I, Roodbari F. Prolonged incubation of processed human spermatozoa will increase DNA fragmentation. Andrologia. 2014;46(4):374-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Yun JI, Gong SP, Song YH, Lee ST. Effects of combined antioxidant supplementation on human sperm motility and morphology during sperm manipulation in vitro. Fertil Steril. 2013;100(2):373-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Lenzi A, Sgro P, Salacone P, Paoli D, Gilio B, Lombardo F, et al. A placebo-controlled double-blind randomized trial of the use of combined L-carnitine and l-acetyL-carnitine treatment in men with asthenozoospermia. Fertil Steril. 2004;81(6):1578-84. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Lee YJ, Lee SH, Kim YJ, Hwangbo Y, Lee S, Cheong HT, et al. Effects of L-carnitine and Nicotinic Acid on Sperm Characteristics in Miniature Pigs. Reprod Devel Biol. 2016;40(1):1-5. [View at Publisher] [DOI] [Google Scholar]
10. De Rosa M, Boggia B, Amalfi B, Zarrilli S, Vita A, Colao A, et al. Correlation between seminal carnitine and functional spermatozoal characteristics in men with semen dysfunction of various origins. Drugs R D. 2005;6(1):1-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Galarza DA, López‐Sebastián A, Santiago‐Moreno J. Supplementing a skimmed milk-egg yolk‐based extender with L‐carnitine helps maintain the motility, membrane integrity and fertilizing capacity of chilled ram sperm. Reprod Domest Anim. 2020:55(7):805-13. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Nery Igor HAV, Araújo Silva RAJ, Souza HM, Arruda LCP, Monteiro MM, Seal DCM, et al. Effects of l-carnitine on equine semen quality during liquid storage. Biopreserv Biobank. 2020;18(5):403-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Jiang XH, Jiang C, Yu L, Li XL, Zuo T, Gu PF, et al. Supplementation with L-carnitine rescues sperm epigenetic changes in asthenospermic male semen with altered Acetyl-L-carnitine levels. Reprod Dev Med. 2020;4(3):146-58. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Banihani S, Sharma R, Bayachou M, Sabanegh E, Agarwal A. Human sperm DNA oxidation, motility and viability in the presence of l‐carnitine during in vitro incubation and centrifugation. Andrologia. 2012;44(S1):505-12. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem. 2005:53(6):1841-56. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Fernández JL, Muriel L, Goyanes V, Segrelles E, Gosálvez J, Enciso M, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil Steril. 2005;84(4):833-42. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Check ML, Kiefer D, Check JH, Hourani W, Long R. Treatment of sperm with subnormal host scores with chymotrypsin/viable pregnancy after IUI. Arch Androl. 2002;48(2):155-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Czinner E, Hagymasi K, Blazovics A, A Kery, É Szőke, Lemberkovics E. The in vitro effect of Helichrysi flos on microsomal lipid peroxidation. J Ethnopharmacol. 2001:77(1):31-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Mayorga-Torres BJM, Cardona-Maya W, Cadavid A, Camargo M. Evaluation of sperm functional parameters in normozoospermic infertile individuals. Actas Urol Esp. 2013;37(4):221-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Malmir M, Naderi Noreini S, Ghafarizadeh A, Faraji T, Asali Z. Ameliorative effect of melatonin on apoptosis, DNA fragmentation, membrane integrity and lipid peroxidation of spermatozoa in the idiopathic asthenoteratospermic men: In vitro. Andrologia. 2021;53(2):e13944. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Ghafarizadeh AA, Malmir M, Naderi Noreini S, Faraji T, Ebrahimi Z. The effect of vitamin E on sperm motility and viability in asthenoteratozoospermic men: In vitro study. Andrologia. 2020;53(1):e13891. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Naderi Noreini S, Malmir M, Ghafarizadeh A, Faraji T, Bayat R. Protective effect of L‐carnitine on apoptosis, DNA fragmentation, membrane integrity and Lipid peroxidation of spermatozoa in the asthenoteratospermic men. Andrologia. 2021;53(2):e13932. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Fanaei H, Khayat S, Halvaei I, Ramezani V, Azizi Y, Kasaeian A, et al. Effects of ascorbic acid on sperm motility, viability, acrosome reaction and DNA integrity in teratozoospermic samples. Iran J Reprod Med. 2014:12(2):103-10. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Aitken RJ, Bronson R, Smith TB, De Iuliis GN. The source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies. Mol Hum Reprod. 2013;19(8):475-85. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Kobayashi H, Gil‐Guzman E, Mahran AM, Rakesh, Nelson DR, Thomas Jr AJ, et al. Quality control of reactive oxygen species measurement by luminol‐dependent chemiluminescence assay. J Androl. 2001;22(4):568-74. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Bravo A, Quilaqueo N, Jofré I, Villegas JV. Overtime expression of plasma membrane and mitochondrial function markers associated with cell death in human spermatozoa exposed to nonphysiological levels of reactive oxygen species. Andrologia. 2021:53(2):e13907. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Mahfouz RZ, du Plessis SS, Aziz N, Sharma R, Sabanegh E, Agarwal A. Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress. Fertil Steril. 2010;93(3):814-21. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Al-Dujaily S, Al-Sultani YK, Alddin NNS. DNA normality following in vitro sperm preparation with pentoxifylline and L-carnitine for asthenozoospermic infertile men. Global Journal of Medical Research 2014;13(K7). [View at Publisher] [Google Scholar]
29. Casillas ER, Chaipayungpan S. The distribution of carnitine and acetylcarnitine in the rabbit epididymis and the carnitine content of rabbit spermatozoa during maturation. J Reprod Fertil 1979;56(2):439-44. [View at Publisher] [DOI] [PMID] [Google Scholar]
30. Steiber A, Kerner J, Hoppel CL. Carnitine: a nutritional, biosynthetic, and functional perspective. Mol Aspects Med. 2004;25(5-6):455-73. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Gulcin İ. Antioxidant and antiradical activities of L-carnitine. Life Sci. 2006;78(8):803-11. [View at Publisher] [DOI] [PMID] [Google Scholar]
32. Nateghian Z, Nasr-Esfahani MH, Talaei-Khozani T, Tavalaee M, Aliabadi E. L-carnitine and pentoxifylline supplementation improves sperm viability and motility at low temperature. Int J Fertil Steril. 2023;17(1):61-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
33. Chang D, Kong F, Jiang W, Li F, Zhang C, Ding H, et al. Effects of l-carnitine Administration on Sperm and Sex Hormone Levels in a Male Wistar Rat Reproductive System Injury Model in a High-Altitude Hypobaric Hypoxic Environment. Reprod Sci. 2023;30(7):2231-47. [View at Publisher] [DOI] [PMID] [Google Scholar]
34. Ghorbani F, Nasiri Z, Koohestanidehaghi Y, Lorian K. The antioxidant roles of L-carnitine and Nacetyl cysteine against oxidative stress on human sperm functional parameters during vitrification. Clin Exp Reprod Med. 2021;48(4):316-21. [View at Publisher] [DOI] [PMID] [Google Scholar]
35. Zhang X, Cui Y, Dong L, Sun M, Zhang Y. The efficacy of combined l-carnitine and l-acetyl carnitine in men with idiopathic oligoasthenoteratozoospermia: a systematic review and meta-analysis. Andrologia. 2020;52(2):e13470. [View at Publisher] [DOI] [PMID] [Google Scholar]
36. Altyeb YH, Absy G, Medan MS, Hassan ST, Elsayed DH. The effects of cysteine and L-carnitine on the DNA integrity of post-thaw sperm of frozen buck semen. Iran J Vet Res. 2022;23(3):196-203. [View at Publisher] [DOI] [PMID] [Google Scholar]
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