Volume 7, Issue 2 (7-2019)
Jorjani Biomed J 2019, 7(2): 11-20 |
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Ghaderi S, Maleki M. Histone Methylation in the CYP11A1 Gene Promoter in the Follicular Granulosa Cells Extracted from the Women Referring to theFertility Treatment Clinic in Tabriz, Iran. Jorjani Biomed J 2019; 7 (2) :11-20
URL: http://goums.ac.ir/jorjanijournal/article-1-638-en.html
URL: http://goums.ac.ir/jorjanijournal/article-1-638-en.html
1- Department of biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran
2- Department of biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran , Maleki.masoud@gmail.com
2- Department of biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran , Maleki.masoud@gmail.com
Abstract: (4572 Views)
Infertility is defind as the inability to conceive after one year of regular unprotected sexual intercourse by a couple. Female infertility can have different causes that all factors can in somehow be influenced by genetic factors. Studies have shown that epigenetic changes play an important role in fetal development, oogenesis and spermatogenesis. During the growth of oocyte, follicular cells make a multilayer coating of granulosa cells. Granulosa cells are affected by gonadotropin hormones. The CYP11A1 gene is one of the genes involved in the production of steroid hormones in luteinized granulosa cells. The CYP11A1 enzyme in the progesterone prodaction path way leads to the conversion of cholesterol to pregnenolon. Progesterone is an important steroid hormone that plays an important role in fertility and pregnancy. Histone modifications help to express the CYP11A1 gene. Trimethylation of Lysine 4 on histone H3 (H3K4me3) works to active transcription in the CYP11A1 promoterIn the present study, the level of methylation H3K4me3 in regulation area of CYP11A1 gene in granulosa cells collected from the women with infertility problem and also from fertile women giving oocyte was investigated in Tabriz Jihad Daneshgahi infertilization center. To do this, the Chromatin Immunoprecipitation and then Real-Time PCR were used to investigate the level of methylation.According to the results of the present study, the level of methylation H3K4me3 in the regulating area of CYP11A1 gene in the given infertile people doesn’t show significant difference in comparision with control group and no significant relationship was observed between methylation of histone in CYP11A1 promoter and number of follicles and oocyte.It is suggested that epigenetic changes in regulating area of CYP11A1 gene are not involved in the number of follicle and oocytes.
Type of Article: Original article |
Subject:
Molecular Sciences
Received: 2019/01/19 | Accepted: 2019/06/6 | Published: 2019/07/1
Received: 2019/01/19 | Accepted: 2019/06/6 | Published: 2019/07/1
References
1. Novak B. Gyncology. 2012.
2. Hull MG, Fleming CF, Hughes AO, McDermott A. Theage-related decline in female fecundity: a quantitative controlled study of implanting capacity and survival of individual embryos after in vitro fertilization. Fertility and sterility. 1996;65(4):783-90. 3. Shelling AN. Premature ovarian failure. Reproduction (Cambridge, England). 2010;140(5):633-41. [DOI] [Google Scholar]
3. Nardo LG, Patchava S, Laing I. Polycystic ovary syndrome: pathophysiology, molecular aspects and clinical implications. Panminerva medica. 2008;50(4):267-78. [Google Scholar]
4. Giudice LC, Kao LC. Endometriosis. Lancet (London, England). 2004;364(9447):1789-99. [DOI] [Google Scholar]
5. MH. K. Principles of Human Genetics and Hereditary disease.474-528.
6. Gu L, Wang Q, Sun QY. Histone modifications during mammalian oocyte maturation: dynamics, regulation and functions. Cell cycle (Georgetown, Tex). 2010;9(10):1942-50. [DOI] [Google Scholar]
7. Kageyama S, Liu H, Kaneko N, Ooga M, Nagata M, Aoki F. Alterations in epigenetic modifications during oocyte growth in mice. Reproduction (Cambridge, England). 2007;133(1):85-94. [DOI] [Google Scholar]
8. Hiura H, Obata Y, Komiyama J, Shirai M, Kono T. Oocyte growth-dependent progression of maternal imprinting in mice. Genes to cells : devoted to molecular & cellular mechanisms. 2006;11(4):353-61. [DOI] [Google Scholar]
9. Lestari SW, Rizki MD. Epigenetic: A new approach to etiology of infertility. Medical Journal of Indonesia. 2016;25(4):255-62. [Google Scholar]
10. Johnson AL, BridghamJT, Witty JP, Tilly JL. Susceptibility of avian ovarian granulosa cells to apoptosis is dependent upon stage of follicle development and is related to endogenous levels of bcl-xlong gene expression. Endocrinology. 1996;137(5):2059-66. [DOI] [Google Scholar]
11. Lee L, Asada H, Kizuka F, Tamura I, Maekawa R, Taketani T, et al. Changes in histone modification and DNA methylation of the StAR and Cyp19a1 promoter regions in granulosa cells undergoing luteinization during ovulation in rats. Endocrinology. 2013;154(1):458-70. [DOI] [Google Scholar]
12. Vialou V. [Histone acetylation, gene regulation and depression]. Medecine sciences : M/S. 2010;26(5):465-7. [DOI] [Google Scholar]
13. Peterson CL, Laniel MA. Histones and histone modifications. Current biology : CB. 2004;14(14):R546-51. [DOI] [Google Scholar]
14. Monteiro JB, Colon-Diaz M, Garcia M, Gutierrez S, Colon M, Seto E, et al. Endometriosis is characterized by a distinct patternof histone 3 and histone 4 lysine modifications. Reproductive sciences (Thousand Oaks, Calif). 2014;21(3):305-18. [DOI] [Google Scholar]
15. Cui P, Liu W, Zhao Y, Lin Q, Zhang D, Ding F, et al. Comparative analyses of H3K4 and H3K27 trimethylations between the mouse cerebrum and testis. Genomics, proteomics & bioinformatics. 2012;10(2):82-93. [DOI] [Google Scholar]
16. Tanaka N, Espey LL, Kawano T, Okamura H. Comparison of inhibitory actions of indomethacin and epostane on ovulation in rats. The American journal of physiology. 1991;260(2 Pt 1):E170-4. [DOI] [Google Scholar]
17. Lydon JP, DeMayo FJ, Funk CR, Mani SK, Hughes AR, Montgomery CA, Jr., et al. Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities. Genes & development. 1995;9(18):2266-78. [DOI] [Google Scholar]
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