Volume 6, Issue 4 (12-2018)                   Jorjani Biomed J 2018, 6(4): 1-7 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahmadi A, Soleimani N, Abedini P. Anti -Bacterial Activity of Punica granatum Flower Extract against Several Gram-Negative and Positive Bacteria. Jorjani Biomed J. 2018; 6 (4) :1-7
URL: http://goums.ac.ir/jorjanijournal/article-1-606-en.html
1- Department of Plant Biology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
2- Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran. , soleimani_neda86@yahoo.com
Abstract:   (1151 Views)
Background and objectives: Bacterial antibiotic resistance is a major issue in the process of infectious disease treatments. The aim of this study was an evaluation of the antibacterial activity of Punica granatum flower extract against several gram-negative and positive clinical bacterial isolates.
 
Methods: An adequate dried flower of an endemic mature Punica granatum plant was used for extraction. The standard strain of several gram negative and positive bacteria was chosen for this study, as well as some distinguished clinical strains such as Pseudomonas aeruginosa and Enterococcus spp. In order to indicate the antibacterial effect of Punica granatum mature flower, well-diffusion method was done for each bacterium of the extraction of the flower, so that zone inhibitions can be reported. MIC and MBC test was done.
 
Results: Disc diffusion test was done and the greatest zone inhibition Shigella was 39 mm and then Salmonella typhimurium 13.1 mm. The lowest antibacterial effect of P. granatum extraction was gained on Proteus with 6 mm of zone inhibition. The Highest MIC and MBC effect was obtained from antibacterial evaluation on S. typhimurium and S. epidermidis.
 
Conclusion: the antibacterial activities of medicinal plants, pharmaceutical companies are just using medicinal plants in association with synthetic drugs in order to obtain better results. Setting up a more analytic test on medicinal plants same as HPLC test could be the next stage of this study in order to reach to a higher reliance of medicinal plants antibacterial activities qualification So That we could combine them with synthetic drugs and improve their efficiency.
Full-Text [PDF 422 kb]   (342 Downloads)    
Type of Article: Original article | Subject: General medicine
Received: 2018/11/3 | Revised: 2020/01/26 | Accepted: 2018/12/15

References
1. Rayner C, Munckhof WJ. Antibiotics currently used in the treatment of infections caused by Staphylococcus aureus. Intern Med J. 2005 [DOI:10.1111/j.1444-0903.2005.00976.x]
2. 35 (2): 3-16. Review. Erratum in: Intern Med J. 2006 Feb;36(2):142-3. [DOI:10.1111/j.1445-5994.2005.01058.x]
3. Allen, H. K., J. Donato, H. H. Wang, K. A. Cloud-Hansen, J. E. Davies, and J. Handelsman. .Call of the wild: antibiotic resistance genes in natural environments. Nat. Rev. Microbiol. 2010: 8 :251-259. [DOI:10.1038/nrmicro2312]
4. Llor C, Bjerrum L. Antimicrobial resistance: risk associated with antibiotic overuse and initiatives to reduce the problem. Ther Adv Drug Saf. 2014; 5(6): 229-241. [DOI:10.1177/2042098614554919]
5. Aminov, R. I. . The role of antibiotics and antibiotic resistance in nature. Environ. Microbiol. 2009: 11:2970-2988. [DOI:10.1111/j.1462-2920.2009.01972.x]
6. Friedman M. Antibiotic-resistant bacteria: prevalence in food and inactivation by food-compatible compounds and plant extracts. J Agric Food Chem. 2015: 63(15):3805-22. [DOI:10.1021/acs.jafc.5b00778]
7. Tenover FC. Mechanisms of antimicrobial resistance in bacteria. Am J Infect Control. 2006; 34(5):3-10 [DOI:10.1016/j.ajic.2006.05.219]
8. Brown JC, Jiang X. Prevalence of antibiotic-resistant bacteria in herbal products. J Food Prot. 2008;71(7):1486- 90. [DOI:10.4315/0362-028X-71.7.1486]
9. Huang TH, Peng G, Kota BP, Li GQ, Yamahara J, Roufogalis BD, Li Y. Antidiabetic action of Punica granatum flower extract: activation of PPAR gamma and identification of an active component. Toxicol Appl Pharmacol. 2005; 1; 207 (2): 160-9. [DOI:10.1016/j.taap.2004.12.009]
10. Li Y, Wen S, Kota BP, Peng G, Li GQ, Yamahara J, Roufogalis BD. Punica. granatum flower extract, a potent alphaglucosidase inhibitor, improves postprandial hyperglycemia in Zucker diabetic fatty rats. J Ethnopharmacol. 2005: 3;99(2):239-44. [DOI:10.1016/j.jep.2005.02.030]
11. Kaur G, Jabbar Z, Athar M, Alam MS. Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food Chem Toxicol. 2006; 44(7):984-93. [DOI:10.1016/j.fct.2005.12.001]
12. Nasiri E, Hosseinimehr SJ, Akbari J, Azadbakht M, Azizi S. The Effects of Punica granatum Flower Extract on Skin Injuries Induced by Burn in Rats. Adv Pharmacol Sci. 2017;2017:3059745. [DOI:10.1155/2017/3059745]
13. B. Howell A, H. D'Souza D. The Pomegranate: Effects on Bacteria and Viruses That Influence Human Health. Evid Based Complement Alternat Med. 2013; 60: 6212. [DOI:10.1155/2013/606212]
14. Daneshmandi S, Soleimani N, Pourfathollah AA, Sattari M. Evaluation of the drug synergistic and antibacterial effects of Cuminum cyminum essential oil. Arak Medical University Journal 13 (2), 75-82.
15. Zafarghandi AS, Ashtiani HRA, Rezayat SM, Soleimani N, Doost SRH, Evaluation of Reciprocal Pharmaceutical Effects and Antibacterial Activity of Silver Nanoparticles and Methanolic Extract of Crocus sativus L.(Saffron) on Some Bacterial Strains. Int J Enteric Pathog 5 (1), 18-23. [DOI:10.15171/ijep.2017.05]
16. soleimani N, ebraze N. Evaluate antibacterial effects of cinnamomun verum and ferula gummosa essential oil on some pathogen gram positive and negative bacteria. New cellular & molecular biotechnology journal. 2014: 6 (23), 87- 93.
17. Haghighi F, Roudbar Mohammadi S, Soleimani N, Sattari M. Evaluation of antifungal activity of essential oils of Thymus vulgaris, Petroselinum Crispum, Cuminum cyminum and Bunium persicum on candida albicans in comparison with Fluconazole Modares Journal of Medical Sciences: Pathobiology. 2010: 14 (1), 29-35.
18. Soleimani N, Sattari M, Sepehriseresht S, Daneshmandi S. Evaluation of reciprocal pharmaceutical effects and antibacterial activity of Bunium persicum essential oil against some Gram positive and Gram negative bacteria. Iran J Med Microbiol. 26-34.
19. Soleimani N, Mobarez A, Jafari Olia M, Atyabi F. Synthesis, Characterization and Effect of the Antibacterial Activity of Chitosan Nanoparticles on VancomycinResistant Enterococcus and Other Gram Negative or Gram Positive Bacteria. International Journal of Pure & Applied Sciences & Technology. 2015: 26(1): 14- 23.
20. Vahid Dastjerdi E, Abdolazimi Z, Ghazanfarian M, Amdjadi P, Kamalinejad M, Mahboubi A. Effect of Punica granatum L. Flower Water Extract on Five Common Oral Bacteria and Bacterial Biofilm Formation on Orthodontic Wire. Iran J Public Health. 2014; 43(12):1688- 94.
21. Al Laham ShA, Al Fadel FM. The AntiBacterial Effect of Punica granatum Extracts Against Antibiotic Resistant Pasteurella haemolytica. Jundishapur J Microbiol. 2013; 6(9): e7750. [DOI:10.5812/jjm.7750]
22. antibacterial activity of rind and flowers of punica granatum linn, naqvi shAH. J Sci. I. R. Iran. 1993; 4 (1): 1-3.
23. John M ,et al ,.In vitro Activity of cinamomum zeylanicum Against azole resistant and sensitive candida species and apilot study of cinnamon for oral candidiasis,The American Journal of Chinese medicine,January 1996,Vol.24,No.02:pp:103-109. [DOI:10.1142/S0192415X96000153]
24. Preuss HG, Echard B, Brook I, Elliott TB. Minimum inhibitory concentrations of herbal essential oils and monolaurin for gram-positive and gram-negative bacteria. Mol Cell Biochem 2005; 272(1-2):29-34. [DOI:10.1007/s11010-005-6604-1]
25. Y.ghasemi et al, Ferula gummosa Frvits:Av Aromatic Antimicrobial Agant,chemistry of natural compounds. 2005: 41 (3): 42-55. [DOI:10.1007/s10600-005-0138-3]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | Jorjani Biomedicine Journal

Designed & Developed by : Yektaweb