Cloning of Minor Autolysin of Streptococcus Pneumoniae

Mahboobi, R,; Fallah Mehrabadi, J,; ,; Pourmand, MR,; Mashhadi, R,; Haddadi, A,

Volume 8, Issue 5 (winter[PERSIAN] 2015) mljgoums 2015, 8(5): 104-110 | Back to browse issues page

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Mahboobi, R, Fallah Mehrabadi, J, Pourmand, MR, Mashhadi, R, Haddadi, A. Cloning of Minor Autolysin of Streptococcus Pneumoniae. mljgoums 2015; 8 (5) :104-110
URL: http://mlj.goums.ac.ir/article-1-567-en.html

Cloning of Minor Autolysin of Streptococcus Pneumoniae

Mahboobi, R¹

, Fallah Mehrabadi, J²

, Pourmand, MR

³, Mashhadi, R¹

, Haddadi, A⁴

1- Tehran University of Medical Sciences, Tehran, Iran
2- Lister Microbiology Institute, Tehran, Iran
3- School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , mpourmand@tums.ac.ir
4- Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran

Abstract: (16142 Views)

Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae (ATCC 49619), Specific primers were designed for amplifying minor autolysin gene fragment, using PCR. The purified gene fragment was inserted into pET21a vector and was transformed into bacterial competent cells by heat shock technique. The presence of gene and absence of mutation in the recombinant vector were checked out with sequencing and enzymatic digestion methods. The gene sequence was finally analyzed by bioinformatic tools. Results: The gene of minor autolysin was cloned successfully and the result of enzymatic digestion was the indication of complete isolation of this gen from plasmid. . Bioinformatics studies revealed that the mature protein was lacking signal peptide and the gene encoded 318 amino acids with a molecular weight of 36.4 kDa. Conclusion: The presentation and characterization of novel antigens such as minor autolysin could help us with finding new approaches for preventing and controlling pneumococcal infection. Keywords: Streptococcus Pneumoniae, Minor Autolysin, Cloning

Keywords: Streptococcus Pneumoniae, Minor Autolysin, Cloning

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Research Article: Original Paper |
Received: 2014/09/23 | Accepted: 2014/09/23 | Published: 2014/09/23 | ePublished: 2014/09/23