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Volume 8, Issue 2 (summer 2014[PERSIAN] 2014)
mljgoums 2014, 8(2): 27-32 |
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Zhand S, Tabaraei A, moradi A, Fotoohi F, Javid N, Bazoori M, et al . Detection of Seasonal Influenza H1N1 and H3N2 Viruses using RT-PCR Assay during 2009 Flu Pandemic in Golestan Province . mljgoums 2014; 8 (2) :27-32
URL: http://mlj.goums.ac.ir/article-1-446-en.html
URL: http://mlj.goums.ac.ir/article-1-446-en.html
S Zhand1 
, A Tabaraei2 , A Moradi2 , F Fotoohi3 , N Javid1 , M Bazoori4 , E Haji Mohammadi5 , A Ghaemi 6
, A Tabaraei2 , A Moradi2 , F Fotoohi3 , N Javid1 , M Bazoori4 , E Haji Mohammadi5 , A Ghaemi 6
1- MSc of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
2- Assistant Professor of Virology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3- Assistant Professor of Virology, Influenza Research Center, Pasteur Institute of Iran, Tehran, Iran
4- BSc of Medical Laboratory, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
5- BSc of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
6- Assistant professor of Virology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran ,ghaem_amir@yahoo.com
2- Assistant Professor of Virology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3- Assistant Professor of Virology, Influenza Research Center, Pasteur Institute of Iran, Tehran, Iran
4- BSc of Medical Laboratory, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
5- BSc of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
6- Assistant professor of Virology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran ,
Abstract: (17926 Views)
Abstract
Background and Objective: The emergence of a novel H1N1influenza A virus of animal origin with transmissibility from human to human poses pandemic concern. Current subtypes of Seasonal influenza A viruses spread in human are influenza A H1N1 influenza A H3N2 and influenza type B viruses. The aim of this study was to determine current strains of the H3N2 and new H1N1 subtypes of influenza A virus from patients suspected influenza infection in 2009 flu pandemic in Golestan province, Iran.
Material and Methods: In this descriptive study, respiratory samples (n = 153) from patients with acute respiratory symptoms were collected in 2009 flu pandemic applied during 2009 pandemic influenza in Golestan province. After reverse transcription of extracted viral RNA, PCR was developed for both H1N1and H3N2subtypes using CDC specific primers.
Results: The mean age of patients was 16.59. Of them 45.1% were male. Thirteen (8.49%) were infected with seasonal influenza H1N1 and 25(16.33%) with seasonal H3N2influenza.
Conclusion: The rate of infection with seasonal H1N1and H3N2is similar to other studies reported from Iran, but lower than the rate reported from other parts of the world.
Key Words: Influenza A Virus, H1N1, H3N2, RT-PCR, Iran
Research Article: Original Paper |
Received: 2014/04/5 | Accepted: 2014/04/5 | Published: 2014/04/5 | ePublished: 2014/04/5
Received: 2014/04/5 | Accepted: 2014/04/5 | Published: 2014/04/5 | ePublished: 2014/04/5
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.