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mljgoums 2024, 18(2): 26-28 |
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Zamani S, Besharat S, Behnampour N, Behnam A, Asgari N, Mortazavi N. Detection and identification of Prevotella Melaninogenica in saliva samples of patients with ulcerative colitis. mljgoums 2024; 18 (2) :26-28
URL: http://mlj.goums.ac.ir/article-1-1697-en.html
URL: http://mlj.goums.ac.ir/article-1-1697-en.html
Samin Zamani1 
, Sima Besharat2 , Nasser Behnampour3 , Armina Behnam4 , Negar Asgari5 , Nazanin Mortazavi6
, Sima Besharat2 , Nasser Behnampour3 , Armina Behnam4 , Negar Asgari5 , Nazanin Mortazavi6
1- Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran;Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
2- Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran
3- Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, Iran
4- Dental Research Center, Golestan University of Medical Sciences, Gorgan, Iran
5- Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
6- Dental Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Oral and Maxillofacial Medicine, School of Dentistry, Golestan University of Medical Sciences, Gorgan, Iran ,mortazavi_nazanin@yahoo.com
2- Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran
3- Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, Iran
4- Dental Research Center, Golestan University of Medical Sciences, Gorgan, Iran
5- Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
6- Dental Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Oral and Maxillofacial Medicine, School of Dentistry, Golestan University of Medical Sciences, Gorgan, Iran ,
Abstract: (1965 Views)
Background: Ulcerative colitis (UC) is a long-term inflammatory bowel disease (IBD) caused by abnormal immune responses, leading to inflammation and scarring in the large intestine. The bacteria Prevotella melaninogenica, found in the intestine and mouth, may contribute to UC. This study focuses on the detection of P. melaninogenica in the saliva of UC patients and compares them with the healthy control (HC) group.
Methods: The present study was a case-control study including 40 UC patients and 40 healthy controls (HCs) with an average age of 43.0 3± 10.3. This study used a real-time PCR test to investigate the frequency and average number of P. melaninogenica from the 16S rRNA gene sequence of P. melaninogenica in both groups.
Results: P. melaninogenica was more frequent in UC patients (77.5%) than HCs (45%) (p = 0.003). The patient group had more bacteria (339.31 ± 1082.29) than HCs (61.29 ± 154.03) (p = 0.005). Women in the UC group had more P. melaninogenica (492.35 ± 1427.61) than the control group (56.98 ± 123.50) (p = 0.0342). Similarly, men in the UC group (262.85 ± 664.97) had more bacteria than the control group (72.62 ± 222.76) (p = 0.015).
Conclusion: The current study showed that dysbiosis in P. melaninogenica, a bacterium in human saliva, could be important in the development of UC. Further investigation is needed to evaluate its use as a potential biomarker in the UC.
Methods: The present study was a case-control study including 40 UC patients and 40 healthy controls (HCs) with an average age of 43.0 3± 10.3. This study used a real-time PCR test to investigate the frequency and average number of P. melaninogenica from the 16S rRNA gene sequence of P. melaninogenica in both groups.
Results: P. melaninogenica was more frequent in UC patients (77.5%) than HCs (45%) (p = 0.003). The patient group had more bacteria (339.31 ± 1082.29) than HCs (61.29 ± 154.03) (p = 0.005). Women in the UC group had more P. melaninogenica (492.35 ± 1427.61) than the control group (56.98 ± 123.50) (p = 0.0342). Similarly, men in the UC group (262.85 ± 664.97) had more bacteria than the control group (72.62 ± 222.76) (p = 0.015).
Conclusion: The current study showed that dysbiosis in P. melaninogenica, a bacterium in human saliva, could be important in the development of UC. Further investigation is needed to evaluate its use as a potential biomarker in the UC.
Research Article: Research Article |
Subject:
Microbiology
Received: 2023/08/1 | Accepted: 2023/11/1 | Published: 2024/03/30 | ePublished: 2024/03/30
Received: 2023/08/1 | Accepted: 2023/11/1 | Published: 2024/03/30 | ePublished: 2024/03/30
References
1. Gajendran M, Loganathan P, Jimenez G, Catinella AP, Ng N, Umapathy C, et al. A comprehensive review and update on ulcerative colitis. Dis Mon. 2019; 65(12): 100851. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Halling ML, Kjeldsen J, Knudsen T, Nielsen J, Hansen LK. Patients with inflammatory bowel disease have increased risk of autoimmune and inflammatory diseases. World J Gastroenterol. 2017; 23(33): 6137-6146. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Aniwan S, Santiago P, Loftus EV Jr, Park SH. The epidemiology of inflammatory bowel disease in Asia and Asian immigrants to Western countries. United European Gastroenterol J. 2022; 10(10): 1063-1076. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Lynch WD, Hsu R. Ulcerative Colitis. 2023 Jun 5. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023. [View at Publisher] [PMID]
5. Olfatifar M, Zali MR, Pourhoseingholi MA, Balaii H, Ghavami SB, Ivanchuk M, et al. The emerging epidemic of inflammatory bowel disease in Asia and Iran by 2035: A modeling study. BMC Gastroenterol. 2021; 21(1): 204. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Segal JP, LeBlanc JF, Hart AL. Ulcerative colitis: an update. Clin Med (Lond). 2021; 21(2): 135-139. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Langholz E, Munkholm P, Nielsen OH, Kreiner S, Binder V. Incidence and prevalence of ulcerative colitis in Copenhagen county from 1962 to 1987. Scand J Gastroenterol. 1991; 26(12): 1247-56. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Lepage P, Häsler R, Spehlmann ME, Rehman A, Zvirbliene A, Begun A, et al. Twin study indicates loss of interaction between microbiota and mucosa of patients with ulcerative colitis. Gastroenterology. 2011; 141(1): 227-36. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Guo XY, Liu XJ, Hao JY. Gut microbiota in ulcerative colitis: insights on pathogenesis and treatment. J Dig Dis. 2020; 21(3): 147-159. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Ribaldone DG, Brigo S, Mangia M, Saracco GM, Astegiano M, Pellicano R. Oral Manifestations of Inflammatory Bowel Disease and the Role of Non-Invasive Surrogate Markers of Disease Activity. Medicines (Basel). 2020; 7(6): 33. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Veloso FT. Extraintestinal manifestations of inflammatory bowel disease: do they influence treatment and outcome? World J Gastroenterol. 2011; 17(22): 2702-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Kitamoto S, Nagao-Kitamoto H, Hein R, Schmidt TM, Kamada N. The Bacterial Connection between the Oral Cavity and the Gut Diseases. J Dent Res. 2020; 99(9): 1021-1029. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Li C, Wu Y, Xie Y, Zhang Y, Jiang S, Wang J, et al. Oral manifestations serve as potential signs of ulcerative colitis: A review. Front Immunol. 2022; 13: 1013900. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Könönen E, Gursoy UK. Oral Prevotella Species and Their Connection to Events of Clinical Relevance in Gastrointestinal and Respiratory Tracts. Front Microbiol. 2022; 12: 798763. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Könönen E, Fteita D, Gursoy UK, Gursoy M. Prevotella species as oral residents and infectious agents with potential impact on systemic conditions. J Oral Microbiol. 2022; 14(1): 2079814. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Kaan AMM, Kahharova D, Zaura E. Acquisition and establishment of the oral microbiota. Periodontol 2000. 2021; 86(1): 123-141. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Kondo Y, Sato K, Nagano K, Nishiguchi M, Hoshino T, Fujiwara T, et al. Involvement of PorK, a component of the type IX secretion system, in Prevotella melaninogenica pathogenicity. Microbiol Immunol. 2018; 62(9): 554-566. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Qi Y, Zang SQ, Wei J, Yu HC, Yang Z, Wu HM, et al. High-throughput sequencing provides insights into oral microbiota dysbiosis in association with inflammatory bowel disease. Genomics. 2021; 113(1 Pt 2): 664-676. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Martin FE, Nadkarni MA, Jacques NA, Hunter N. Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol. 2002; 40(5): 1698-704. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Sharma G, Garg N, Hasan S, Shirodkar S. Prevotella: An insight into its characteristics and associated virulence factors. Microb Pathog. 2022; 169: 105673. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Ren W, Yan H, Yu B, Walsh MC, Yu J, Zheng P, et al. Prevotella-rich enterotype may benefit gut health in finishing pigs fed diet with a high amylose-to-amylopectin ratio. Anim Nutr. 2021; 7(2): 400-411. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Larsen JM. The immune response to Prevotella bacteria in chronic inflammatory disease. Immunology. 2017 Aug;151(4):363-374. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Belkaid Y, Hand TW. Role of the microbiota in immunity and inflammation. Cell. 2014 Mar 27;157(1):121-41. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Hajishengallis G. Periodontitis: from microbial immune subversion to systemic inflammation. Nat Rev Immunol. 2015; 15(1): 30-44. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Atarashi K, Suda W, Luo C, Kawaguchi T, Motoo I, Narushima S, et al. Ectopic colonization of oral bacteria in the intestine drives TH1 cell induction and inflammation. Science. 2017; 358(6361): 359-365. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Molinero N, Taladrid D, Zorraquín-Peña I, de Celis M, Belda I, Mira A, Bartolomé B, et al. Ulcerative Colitis Seems to Imply Oral Microbiome Dysbiosis. Curr Issues Mol Biol. 2022; 44(4): 1513-1527. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Zhang T, Kayani MUR, Hong L, Zhang C, Zhong J, Wang Z, et al. Dynamics of the Salivary Microbiome During Different Phases of Crohn's Disease. Front Cell Infect Microbiol. 2020; 10: 544704. [View at Publisher] [DOI] [PMID] [Google Scholar]
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