Wed, Apr 16, 2025
Volume 18, Issue 3 (May-Jun 2024)
mljgoums 2024, 18(3): 13-15 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Michel T, Esther V, Bernard B, Louise M N, Simon Stevensen S, Raissa N et al . Chemiluminescence vs hemagglutination assay in the detection of Anti-Treponema pallidum antibodies. mljgoums 2024; 18 (3) :13-15
URL: http://mlj.goums.ac.ir/article-1-1590-en.html
URL: http://mlj.goums.ac.ir/article-1-1590-en.html
Toukam Michel1 
, Voundi-Voundi Esther2 , Bole Bernard3 , Mviena Nyimbe Louise4 , Sahmo Simon Stevensen4 , Nogue Raissa4 , Kasia Jean Marie5
, Voundi-Voundi Esther2 , Bole Bernard3 , Mviena Nyimbe Louise4 , Sahmo Simon Stevensen4 , Nogue Raissa4 , Kasia Jean Marie5
1- Department of Microbiology, Parasitology, Hematology and infectious diseases, Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Cameroon , mtoukam2002@gmail.com
2- Department of Microbiology, Parasitology, Hematology and infectious diseases, Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Cameroon
3- Ministry of Public Health, Cameroon
4- Gynaecological Endoscopic Surgery and Human Reproductive Teaching Hospital, Cameroon
5- Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Cameroon
2- Department of Microbiology, Parasitology, Hematology and infectious diseases, Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Cameroon
3- Ministry of Public Health, Cameroon
4- Gynaecological Endoscopic Surgery and Human Reproductive Teaching Hospital, Cameroon
5- Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Cameroon
Abstract: (1248 Views)
Background: Syphilis has serious consequences for human health and poses challenges for biological diagnosis. Techniques such as chemiluminescence are still poorly known in our context. Our objective was to compare chemiluminescence and hemagglutination assays with immunoblot as a reference test for detecting anti-Treponema pallidum antibodies.
Methods: Anti-Treponema pallidum antibodies were detected in serum samples. Comparison of chemiluminescence and hemagglutination assays was carried out with immunoblot as the reference test, based on sensitivity, specificity, positive and negative predictive values, and kappa statistic, using IBM-SPSS version 24 software. The significance level was set at 5%.
Results: A total of 600 participants were recruited, with a prevalence of syphilis infection of 3%. Sensitivity and specificity were 100% and 96.2% for chemiluminescence, and 93.8% and 100% for hemagglutination, respectively. Cohen's kappa statistic between the two tests showed satisfactory agreement (0.768). Cohen's kappa agreements between chemiluminescence/immunoblot and plate hemagglutination assay/immunoblot were greater than 0.8, indicating excellent agreement with the reference test (p<0.0001).
Conclusion: Chemiluminescence is more sensitive but less specific than hemagglutination. The use of chemiluminescence as a first-line test for the diagnosis of syphilis should be considered.
Methods: Anti-Treponema pallidum antibodies were detected in serum samples. Comparison of chemiluminescence and hemagglutination assays was carried out with immunoblot as the reference test, based on sensitivity, specificity, positive and negative predictive values, and kappa statistic, using IBM-SPSS version 24 software. The significance level was set at 5%.
Results: A total of 600 participants were recruited, with a prevalence of syphilis infection of 3%. Sensitivity and specificity were 100% and 96.2% for chemiluminescence, and 93.8% and 100% for hemagglutination, respectively. Cohen's kappa statistic between the two tests showed satisfactory agreement (0.768). Cohen's kappa agreements between chemiluminescence/immunoblot and plate hemagglutination assay/immunoblot were greater than 0.8, indicating excellent agreement with the reference test (p<0.0001).
Conclusion: Chemiluminescence is more sensitive but less specific than hemagglutination. The use of chemiluminescence as a first-line test for the diagnosis of syphilis should be considered.
Research Article: Research Article |
Subject:
Laboratory Sciences
Received: 2022/10/28 | Accepted: 2024/01/21 | Published: 2024/11/12 | ePublished: 2024/11/12
Received: 2022/10/28 | Accepted: 2024/01/21 | Published: 2024/11/12 | ePublished: 2024/11/12
References
1. WHO. Sexually transmitted infections. 2023. [View at Publisher]
2. Buder S, Schöfer H, Meyer T, Bremer V, Kohl PK, Skaletz-Rorowski A, et al. Bacterial sexually transmitted infections. J Dtsch Dermatol Ges. 2019; 17(3): 287-315. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. McClintock HF, Dulak SL. Intimate partner violence and Sexually Transmitted Infections among women in Sub-Saharan Africa. J Immigr Minor Health. 2021; 23(2): 191-198. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. WHO. Rapid Syphilis Tests - IRIS. 2019. [View at Publisher]
5. Guntz Ph, North ML. Sérodiagnostic de la syphilis. Rev Francoph Lab. 1997;1997(294):51-8. [View at Publisher] [DOI] [Google Scholar]
6. Matthias J, Klingler EJ, Schillinger JA, Keller G, Wilson C, Peterman TA. Frequency and characteristics of biological false-positive test results for syphilis reported in Florida and New York City, USA, 2013 to 2017. Journal of Clinical Microbiology. 2019; 57(11): e00898-19. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Tao C, Hao X, Xu W, Zhang J, Pan S, Tao Z, et al. Evaluation of the Elecsys syphilis immunoassay for routine screening of serum samples in China. Sci Rep. 2017; 7(1): 9559. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Li Z, Feng Z, Liu P, Yan C. Screening for antibodies against Treponema pallidum with chemiluminescent microparticle immunoassay: analysis of discordant serology results and clinical characterization. Ann Clin Biochem. 2016; 53(5): 588 92. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Zhiyan L, Meiling W, Ping L, Jinhua D, Zhenlin Y, Zhenru F. Consistency Between Treponema pallidum Particle Agglutination Assay and Architect Chemiluminescent Microparticle Immunoassay and Characterization of Inconsistent Samples. J Clin Lab Anal. 2015; 29(4): 281 4. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Mo X, Jin Y, Yang Y, Hu W, Gu W. Evaluation of a new chemiluminescence immunoassay for diagnosis of syphilis. Eur J Med Res. 2010; 15(2): 66 9. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Chen Q, An J, Rao C, Wang T, Li D, Feng S, Tao C. Performance Evaluation of a Novel Chemiluminescence Assay Detecting Treponema Pallidum Antibody as a Syphilis Screening Method. Clin Lab. 2016; 62(4): 519-26. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Bopage RI, Vollmer-Conna U, Shand AW, Post JJ. Sex differences in the significance of isolated reactive treponemal chemiluminescence immunoassay results. Sex Transm Infect. 2018; 94(3): 187 91. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Wang K-D, Xu D-J, Su J-R. Preferable procedure for the screening of syphilis in clinical laboratories in China. Infect Dis (Lond). 2016; 48(1): 26 31. [View at Publisher] [DOI]
14. Jary A, Dienta S, Leducq V, Le Hingrat Q, Cisse M, Diarra AB, et al. Seroprevalence and risk factors for HIV, HCV, HBV and syphilis among blood donors in Mali. BMC Infect Dis. 2019; 19(1): 1064. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Köksal MO, Beka H, Evlice O, Çiftçi S, Keskin F, Başaran S, et al. Syphilis seroprevalence among HIV-infected males in Istanbul, Turkey. Rev Argent Microbiol. 2020; 52(4): 266-271. [View at Publisher] [DOI] [PMID] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.