[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aims and Scope
Content Coverage
Conflict of Interest
Publication Ethics
Indexing Databases::
Editorial Board::
Executive Members::
Instruction to Authors::
Peer Review::
Articles Archive::
Current Issue
All Issues
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 25, Issue 1 (3-2023) ::
J Gorgan Univ Med Sci 2023, 25(1): 47-53 Back to browse issues page
Effect of Six Weeks of High-Intensity Training on mTOR Phosphorylation and Quadriceps Femoris Muscle Features in Healthy Male Rats
Nasrin Alborzian Juneqani1 , Mohammad Fathi *2 , Rahim Mirnasouri3
1- M.Sc student in Exercise Physiology, Department of Physical Education & Sport Sciences, Faculty of Humanities, Lorestan University, Khorramabad, Iran.
2- * Corresponding Author, Associate Professor of Exercise Physiology, Department of Physical Education & Sport Sciences, Faculty of Humanities, Lorestan University, Khorramabad, Iran. , fathi.m@lu.ac.ir
3- Assistant Professor of Exercise Physiology, Department of Physical Education & Sport Sciences, Faculty of Humanities, Lorestan University, Khorramabad, Iran.
Abstract:   (4004 Views)
Background and Objective: Understanding the cellular signaling mechanisms involved in muscle hypertrophy is considered a scientific challenge. Mammalian target of rapamycin (mTOR) is one of the regulatory factors in this process that increases protein synthesis in skeletal muscle through phosphorylation. This study aimed to determine the effect of six weeks of high-intensity interval training (HIIT) on phosphorylated mTOR protein in the quadriceps muscles of adult male Wistar rats.
Methods: In this experimental study, 16 adult male Wistar rats (six weeks old and weighing an average of 190.93±4.97g) were used. The animals were randomly divided into two groups of control and training (n=8). The training group underwent six weeks of HIIT on a treadmill, with five sessions per week. The load was increased during the six weeks from repeating the interval of 30 meters per minute for 30 seconds in the first sessions to eleven repetitions of the interval of 35 meters per minute for 30 seconds at the end of the sixth week, with rest intervals between the intervals at a speed of 13 meters per minute for 60 seconds. The control group did not undergo any training. The mice were anesthetized, and the Vastus lateralis of the quadriceps muscle was extracted. The level of phosphorylated mTOR protein in the quadriceps muscle was measured using the immunohistochemical method.
Results: HIIT significantly increased the levels of mTOR phosphorylation protein in male Wistar quadriceps femoris muscle compared to the control group (P<0.05).
Conclusion: Interval activity can have a positive effect on muscle hypertrophy through mTOR.
 
Keywords: High-Intensity Interval Training [MeSH], mTOR protein [MeSH], Wistar Rats [MeSH]
Article ID: Vol25-06
Full-Text [PDF 889 kb]   (12207 Downloads)    
Type of Study: Original Articles | Subject: Exercise Physiology
References
1. Biglari S, Gaeini AA, Kordi MR, Ghardashi Afousi A. [The Effect of 8 Weeks High-intensity Interval Training on Myostatin and Follistatin Gene Expression in Gastrocnemius Muscle of the Rats]. J Arak Uni Med Sci. 2018; 21(1): 1-10. [Article Persian] [View at Publisher]
2. Vitale JA, Bonato M, La Torre A, Banfi G. The Role of the Molecular Clock in Promoting Skeletal Muscle Growth and Protecting against Sarcopenia. Int J Mol Sci. 2019 Sep; 20(17): 4318. doi: 10.3390/ijms20174318 [DOI] [PubMed]
3. Bar-Peled L, Sabatini DM. Regulation of mTORC1 by amino acids. Trends Cell Biol. 2014 Jul; 24(7): 400-406. doi: 10.1016/j.tcb.2014.03.003 [DOI] [PubMed]
4. Laplante M, Sabatini DM. mTOR signaling in growth control and disease. Cell. 2012 Apr; 149(2): 274-93. doi: 10.1016/j.cell.2012.03.017 [DOI] [PubMed]
5. Nnah IC, Khayati Kh, Dobrowolski R. Cellular metabolism and lysosomal mTOR signaling. Cell Death in Therapy. 2015; 1: 11-22. [Link]
6. Goodman CA. The role of mTORC1 in regulating protein synthesis and skeletal muscle mass in response to various mechanical stimuli. Rev Physiol Biochem Pharmacol. 2014; 166: 43-95. doi: 10.1007/112_2013_17 [DOI] [PubMed]
7. Hoppeler H, Baum O, Lurman G, Mueller M. Molecular mechanisms of muscle plasticity with exercise. Compr Physiol. 2011 Jul; 1(3): 1383-412. doi: 10.1002/cphy.c100042 [DOI] [PubMed]
8. Robinson MM, Dasari S, Konopka AR, Johnson ML, Manjunatha S, Esponda RR, et al. Enhanced Protein Translation Underlies Improved Metabolic and Physical Adaptations to Different Exercise Training Modes in Young and Old Humans. Cell Metab. 2017 Mar; 25(3): 581-92. doi: 10.1016/j.cmet.2017.02.009 [DOI] [PubMed]
9. Estes RR, Malinowski A, Piacentini M, Thrush D, Salley E, Losey C, et al. The Effect of High Intensity Interval Run Training on Cross-sectional Area of the Vastus Lateralis in Untrained College Students. Int J Exerc Sci. 2017 Jan; 10(1): 137-45. [PubMed]
10. Faezi G, Sherafati Moghadam M, Shadmehri S, Fathalipour M. [The effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways gastrocnemius muscle of type 2 diabetic rats]. Medical Science Journal of Islamic Azad University, Tehran Medical Branch. 2020; 30(2): 120-27. doi: 10.29252/iau.30.2.120 [Article in Persian] [View at Publisher] [DOI]
11. Hawley JA, Lundby C, Cotter JD, Burke LM. Maximizing Cellular Adaptation to Endurance Exercise in Skeletal Muscle. Cell Metab. 2018 May; 27(5): 962-76. doi: 10.1016/j.cmet.2018.04.014 [DOI] [PubMed]
12. Fathi M, Gharakhanlou R, Solimani M, Rajabi H, Rezaei R. [The Effect of Resistance Exercise on MyoD Expression in Slow and Fast Muscles of Wistar Rats]. Journal of Sport Biosciences. 2015; 6(4): 435-49. doi: 10.22059/jsb.2015.53217 [Article in Persian] [View at Publisher] [DOI]
13. Kazior Z, Willis SJ, Moberg M, Apró W, Calbet JA, Holmberg HC, et al. Endurance Exercise Enhances the Effect of Strength Training on Muscle Fiber Size and Protein Expression of Akt and mTOR. PLoS One. 2016 Feb; 11(2): e0149082. doi: 10.1371/journal.pone.0149082 [DOI] [PubMed]
14. Wilson JM, Lowery RP, Joy JM, Andersen JC, Wilson SM, Stout JR, et al. The effects of 12 weeks of beta-hydroxy-beta-methylbutyrate free acid supplementation on muscle mass, strength, and power in resistance-trained individuals: a randomized, double-blind, placebo-controlled study. Eur J Appl Physiol. 2014 Jun; 114(6): 1217-27. doi: 10.1007/s00421-014-2854-5 [DOI] [PubMed]
15. Gerth N, Ruoss C, Jakob K, Dobenecker B, Reese S, Starck JM. Effects of an aerobic training and training cessation on skeletal muscle ultrastructure in domestic dogs. J Morphol Sci. 2015. 32(3): 192-99. doi: 10.4322/jms.095515 [View at Publisher] [DOI]
16. Szentesi P, Csernoch L, Dux L, Keller-Pintér A. Changes in Redox Signaling in the Skeletal Muscle with Aging. Oxid Med Cell Longev. 2019 Jan; 2019: 4617801. doi: 10.1155/2019/4617801 [DOI] [PubMed]
17. Ross A, Leveritt M. Long-Term Metabolic and Skeletal Muscle Adaptations to Short-Sprint Training. Sports Med. 2011; 31(15): 1063-82. doi: 10.2165/00007256-200131150-00003 [DOI] [PubMed]
18. Haraguchi FK, de Brito Magalhães CL, Neves LX, dos Santos RC, Pedrosa ML, Silva ME. Whey protein modifies gene expression related to protein metabolism affecting muscle weight in resistance-exercised rats. 2014 Jul-Aug; 30(7-8): 876-81. doi: 10.1016/j.nut.2013.12.007 [DOI] [PubMed]
19. 19. National health and medical research council. Australian code for the care and use of animals for scientific purposes. 8th ed. 2013. [Link]
20. Nemati J, Samadi M, Hadidi V, Makintash B. [Effect of resistance training on mTOR and P70S6K Signaling pathway in skeletal muscle of rats]. Journal of Sport and Exercise Physiology. 2015 Feb; 8(1): 1149-56. [Article in Persian] [View at Publisher]
21. Ranjbar K, Agha-Alinejad H, Shahbazi S, Molanouri Shamsi M. [The Effect of Interval Training and Selenium Nanoparticles on mTOR and LC3 mRNA Expression in Mice Bearing Breast Cancer]. Journal of Sport Biosciences. 2018; 10(2): 177-92. doi: 10.22059/jsb.2017.209626.1092 [Article in Persian] [View at Publisher] [DOI]
22. Lundberg TR, Fernandez-Gonzalo R, Gustafsson T, Tesch PA. Aerobic exercise alters skeletal muscle molecular responses to resistance exercise. Med Sci Sports Exerc. 2012 Sep; 44(9): 1680-88. doi: 10.1249/MSS.0b013e318256fbe8 [DOI] [PubMed]
23. Rose AJ, Bisiani B, Vistisen B, Kiens B, Richter EA. Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type. Am J Physiol Regul Integr Comp Physiol. 2009 Feb; 296(2): R326-33. doi: 10.1152/ajpregu.90806.2008 [DOI] [PubMed]
24. de Souza EO, Tricoli V, Bueno Junior C, Pereira MG, Brum PC, Oliveira EM, Roschel H, Aoki MS, Urginowitsch C. The acute effects of strength, endurance and concurrent exercises on the Akt/mTOR/p70(S6K1) and AMPK signaling pathway responses in rat skeletal muscle. Braz J Med Biol Res. 2013 Apr;46(4):343-7. doi: 10.1590/1414-431x20132557 [DOI] [PubMed]
25. Liao J, Li Y, Zeng F, Wu Y. Regulation of mTOR Pathway in Exercise-induced Cardiac Hypertrophy. Int J Sports Med. 2015 May; 36(5): 343-50. doi: 10.1055/s-0034-1395585 [DOI] [PubMed]
26. Ma Z, Qi J, Meng S, Wen B, Zhang J. Swimming exercise training-induced left ventricular hypertrophy involves microRNAs and synergistic regulation of the PI3K/AKT/mTOR signaling pathway. Eur J Appl Physiol. 2013 Oct; 113(10): 2473-86. doi: 10.1007/s00421-013-2685-9 [DOI] [PubMed]
27. Garcia NF, Sponton AC, Delbin MA, Parente JM, Castro MM, Zanesco A, et al. Metabolic parameters and responsiveness of isolated iliac artery in LDLr-/- mice: role of aerobic exercise training. Am J Cardiovasc Dis. 2017 Apr; 7(2): 64-71. [PubMed]
28. Showkat M, Beigh MA, Andrabi KI. mTOR Signaling in Protein Translation Regulation: Implications in Cancer Genesis and Therapeutic Interventions. Mol Biol Int. 2014; 2014: 686984. doi: 10.1155/2014/686984 [DOI] [PubMed]
29. Hinkley JM, Konopka AR, Suer MK, Harber MP. Short-term intense exercise training reduces stress markers and alters the transcriptional response to exercise in skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2017 Mar; 312(3): R426-R433. doi: 10.1152/ajpregu.00356.2016 [DOI] [PubMed]
30. Martins C, Kazakova I, Ludviksen M, Mehus I, Wisloff U, Kulseng B, et al. High-Intensity Interval Training and Isocaloric Moderate-Intensity Continuous Training Result in Similar Improvements in Body Composition and Fitness in Obese Individuals. Int J Sport Nutr Exerc Metab. 2016 Jun; 26(3): 197-204. doi: 10.1123/ijsnem.2015-0078 [DOI] [PubMed]
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Alborzian Juneqani N, Fathi M, Mirnasouri R. Effect of Six Weeks of High-Intensity Training on mTOR Phosphorylation and Quadriceps Femoris Muscle Features in Healthy Male Rats. J Gorgan Univ Med Sci 2023; 25 (1) :47-53
URL: http://goums.ac.ir/journal/article-1-4115-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 25, Issue 1 (3-2023) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.08 seconds with 36 queries by YEKTAWEB 4710
Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)