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:: Volume 21, Issue 4 (12-2019) ::
J Gorgan Univ Med Sci 2019, 21(4): 86-92 Back to browse issues page
Antimicrobial resistance and prevalence of Metallo-β-lactamase genes IMP-1, VIM-1 and class 1 integron in clinical isolates of Pseudomonas aeruginosa
Fatemeh Namvar1 , Marjan Shaheli 2, Abbasali Rezaeian3
1- M.Sc Student in Genetic, Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran
2- Assistant Professor, Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran , shaheli87@yahoo.com
3- Assistant Professor, Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
Abstract:   (10314 Views)
Background and Objective: The ever-increasing resistance to beta-lactame antibiotic in opportunistic Pseudomonas aeruginosa bacteria considered as one of the important factors of death of hospital-acquired infections. This study was performed for determine the antibiotic resistance and prevalence of IMP-1 and VIM-1 metallo-beta-lactamase and integron class I genes in clinical isolates of Pseudomonas aeruginosa.
Methods: In this descriptive-analytical study, 200 Pseudomonas spp. isolates from blood, urine, ulcer, eye and sputum infections were collected from Arsanjan hospital in Fars province in south –west of Iran during April-September 2016. After confirmation genus of bacterial by biochemical and 16S rRNA tests, and isolation of Pseudomonas aeruginosa by specific primer of lasI Gene, antibiotic susceptibility was done according to diffusion disk assay and CLSI procedure, the presence of blaVIM, blaIMP and Int-1 genes were determined by PCR.
Results: The results of phenotypic and genotypic tests led to the isolation of 107 isolates of Pseudomonas aeruginosa that the highest resistance with (79.38%) for cefepime and the lowest resistance with (13.08%) for tobramycin. Out of 107 isolates, 10 (9.35%) isolates were carrying class1 Integron,19 (17/76%) isolates carrying IMP gene, 23 (21.5%) isolates carrying VIM gene, 4 (3.74%) isolates carrying IMP gene and integron class1, 11 (10.28%) isolates carrying VIM gene, and class1 intgron, 15 (14.02%) isolates carrying both IMP, VIM and 12 (11.22%) isolates simultaneously were carrying each three genes, VIM, IMP and class1 integron. 13 (12.15%) isolates did not have none of these three genes, VIM, IMP, class1 integron.
Conclusion: The results showed increased multidrug resistance and simultaneous presence of one or two IMP, VIM and Int-1 genes in Pseudomonas aeruginosa strains. Int-1 has the ability to transduce resistance genes and create resistant populations.
Keywords: Pseudomonas aeruginosa, Metallo-beta-lactamase, Class1 integron, IMP gene, VIM gene
Full-Text [PDF 343 kb]   (11914 Downloads)    
Type of Study: Original Articles | Subject: Microbiology
References
1. Hemmati F, Soroori Zanjani R, Haghi F, Zeighami H. [Determination of Antibiotic Resistance Profile and Frequency of Metallo-Beta- Lactamases in Pseudomonas Aeruginosa Isolates]. J Adv Med Biomed Res. 2014; 22 (93) :77-85. [Article in Persian]
2. Doosti M, Ramazani A, Garshasbi M. Identification and Characterization of Metallo-β-Lactamases Producing Pseudomonas aeruginosa Clinical Isolates in University Hospital from Zanjan Province, Iran. Iranian Biomedical Journal. 2013; 17 (3): 129-33. doi: 10.6091/ibj.1107.2013
3. Hawkey PM. The origins and molecular basis of antibiotic resistance. BMJ. 1998 Sep; 317(7159): 657-60. doi: 10.1136/bmj.317.7159.657
4. Zeighami H, Haghi F, Hajiahmadi F. [Integrons and Their role in Antibiotic Resistance]. J Med Lab Diagn. 2014; 5(22): 61-71. [Article in Persian]
5. Peymani A, Naserpour Farivar T, Rahimi H, Ranjbar M, Najafipour R. [Frequency of Class I Integron among Multidrug Resistant Pseudomonas aeruginosa Isolates from the Selected Hospitals in Qazvin and Tehran, Iran]. Qom Univ Med Sci J. 2014; 8(3): 61-69. [Article in Persian]
6. Deyhim B, Bessikhasteh M. [The pattern of antibiotic resistance and MBL production of Pseudomonas aeruginosa strains isolated from clinical samples from Dr. Ganjavian hospital in Dezful]. Zistfanavari Microbi, Shahr-e-Qods Branch, Islamic Azad University. 2012; 4(4): 21-28. [Article in Persian]
7. Kazeminezhad B, Bostanmanesh Rad A, Gharib A, Zahedifard S. blaVIM and blaIMP Genes Detection in Isolates of Carbapenem Resistant P. aeruginosa of Hospitalized Patients in Two Hospitals in Iran. Iran J Pathol. 2017; 12(4): 392-96.
8. Sacha P, Wieczorek P, Hauschild T, Zórawski M, Olszańska D, Tryniszewska E. Metallo-beta-lactamases of Pseudomonas aeruginosa--a novel mechanism resistance to beta-lactam antibiotics. Folia Histochem Cytobiol. 2008; 46(2): 137-42. doi: 10.2478/v10042-008-0020-9
9. Mendiratta DK, Deotale V, Narang P. Metallo-beta-lactamase producing Pseudomonas aeruginosa in a hospital from a rural area. Indian J Med Res. 2005 May; 121(5): 701-3.
10. Peymani A, Naserpour Farivar T, Mohammadi Ghanbarlou M, Najafipour R. Dissemination of Pseudomonas aeruginosa producing bla IMP-1 and bla VIM-1 in Qazvin and Alborz educational hospitals, Iran. Iran J Microbiol. 2015 Dec; 7(6): 302-309.
11. Walsh TR. The emergence and implications of metallo-beta-lactamases in Gram-negative bacteria. Clin Microbiol Infect. 2005 Nov; 11(Suppl 6): 2-9. doi: 10.1111/j.1469-0691.2005.01264.x
12. Ramasamy D, Mishra AK, Lagier JC, Padhmanabhan R, Rossi M, Sentausa E, et al. A polyphasic strategy incorporating genomic data for the taxonomic description of novel bacterial species. Int J Syst Evol Microbiol. 2014 Feb; 64(Pt 2): 384-91. doi: 10.1099/ijs.0.057091-0
13. Janda JM, Abbott SL. 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. J Clin Microbiol. 2007 Sep; 45(9): 2761-64. doi: 10.1128/JCM.01228-07
14. Shahcheraghi F, Nikbin VS, Feizabadi MM. Identification and genetic characterization ofmetallo-beta-lactamase-producing strains of Pseudomonas aeruginosa in Tehran, Iran. New Microbiologica. 2010 Jul; 33(3): 243-8.
15. Patel JB, Patel R, Weinstein MP. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third informational Supplement. M100-S23. Clinical and Laboratory Standards Institute. 2013 Jan; vol 33. No 1.
16. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012 Mar; 18(3): 268-81. doi: 10.1111/j.1469-0691.2011.03570.x
17. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012 Mar; 18(3): 268-81. doi: 10.1111/j.1469-0691.2011.03570.x
18. Kohanteb J, Dayaghi M, Motazedian M, Ghayumi MA. Comparison of biotyping and antibiotyping of Pseudomonas aeruginosa isolated from patients with burn wound infection and nosocomial pneumonia in Shiraz, Iran. Pak J Biol Sci. 2007 Jun; 10(11): 1817-22.
19. Nikokar I, Tishayar A, Flakiyan Z, Alijani K, Rehana-Banisaeed S, Hossinpour M, et al. Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa, isolated from burn patients in Guilan, Iran. Iran J Microbiol. 2013 Mar; 5(1): 36-41.
20. Fazeli H, Havaei S, Solgi H, Shokri D, Motallebirad T. Pattern of Antibiotic Resistance in PesudomonasAeruginosa Isolated from Intensive Care Unit, Isfahan, Iran. J Isfahan Med Sch. 2013 Jun; 31(232): 433-38.
21. Martínez T, Vazquez GJ, Aquino EE, Goering RV, Robledo IE. Two novel class I ntegrin arrays containing IMP-18 metallo-β-lactamase gene in Pseudomonas aeruginosa clinical isolates from Puerto Rico. Antimicrob Agents Chemother. 2012; 56(4): 2119-21. doi: 10.1128/AAC.05758-11
22. Toval F, Guzmán-Marte A, Madriz V, Somogyi T, Rodríguez C, García F. Predominance of carbapenem-resistant Pseudomonas aeruginosa isolates carrying blaIMP and blaVIM metallo-β-lactamases in a major hospital in Costa Rica. J Med Microbiol. 2015 Jan; 64(Pt 1): 37-43. doi: 10.1099/jmm.0.081802-0
23. Aghamiri S, Amirmozafari N, Fallah Mehrabadi J, Fouladtan B, Samadi Kafil H. Antibiotic Resistance Pattern and Evaluation of Metallo-Beta Lactamase Genes Including bla- IMP and bla- VIM Types in Pseudomonas aeruginosa Isolated from Patients in Tehran Hospitals. ISRN Microbiol. 2014 Apr; 2014: 941507. doi: 10.1155/2014/941507
24. Keramati N, Zeighami H, Haghi F. [Frequency of Class I and II Integrons in Metalobetalactamase Producing Clinical Isolates of Pseudomonas Aeruginosa]. J Adv Med Biomed Res. 2014; 22(94): 111-19. [Article in Persian]
25. Ohara M, Kouda S, Onodera M, Fujiue Y, Sasaki M, Kohara T, et al. Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan. Microbiol Immunol. 2007; 51(3): 271-77.
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Namvar F, Shaheli M, Rezaeian A. Antimicrobial resistance and prevalence of Metallo-β-lactamase genes IMP-1, VIM-1 and class 1 integron in clinical isolates of Pseudomonas aeruginosa. J Gorgan Univ Med Sci 2019; 21 (4) :86-92
URL: http://goums.ac.ir/journal/article-1-3482-en.html


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Volume 21, Issue 4 (12-2019) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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