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:: Volume 18, Issue 3 (10-2016) ::
J Gorgan Univ Med Sci 2016, 18(3): 92-96 Back to browse issues page
Antimicrobial effect of probiotics in combination with prebiotics against Staphylococcus aureus and Listeria monocytogenes
P Rezaee 1, R Vahedi Shahandashti2 , R Kasra Kermanshahi3
1- Ph.D Candidate in Microbiology, Alzahra University, Tehran, Iran. , parastrezai@gmail.com
2- M.Sc in Microbiology, Alzahra University, Tehran, Iran.
3- Professor, Department of Microbiology, Faculty of Biology Science, Alzahra University, Tehran, Iran.
Abstract:   (7558 Views)

Background and Objective: Probiotc bacteria have benefical effect on consumer health. This study was done to investigate the antimicrobial effect of several probiotic in combinations with different prebiotics against food patoghenic bacteria including Staphylococcus aureus and Listeria monocytogenes.

Methods: In this descriptive - analytical study, probiotics including Lactobacillus plantarum, L. acidophilus, L. fermntum, L. casei and L. rhamnosus with prebiotics (1%) including raffinose, lactulose, inulin and trehalose were cultured in MRS broth for 24 hours at 30ºC in anaerobic conditions. Antimicrobial property of them was determined with well diffusion plate's method.

Results: Probiotics in the presence of prebiotics indicated the higher antimicrobial effect compared to probiotics alone (P<0.05). The application of prebiotics such as L. casei with raffinose showed higher antimicrobial property against Listeria monocytogenes than the free prebiotics consumption. The diameter of inhibitory growth zone in the presence of raffinose as a prebiotics was 14.66 mm and its absence reduced to 11.75 mm.

Conclusion: Antimicrobial effect of probiotics in combination with prebiotics against Staphylococcus aureus and Listeria monocytogenes was higher than probiotics consumption alone.

Keywords: Probiotic, Prebiotic, Antimicrobial Property, Staphylococcus aureus, Listeria monocytogenes
Full-Text [PDF 203 kb] [English Abstract]   (13139 Downloads) |   |   Abstract (HTML)  (987 Views)  
Type of Study: Original Articles | Subject: Microbiology
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Rezaee P, Vahedi Shahandashti R, Kasra Kermanshahi R. Antimicrobial effect of probiotics in combination with prebiotics against Staphylococcus aureus and Listeria monocytogenes. J Gorgan Univ Med Sci 2016; 18 (3) :92-96
URL: http://goums.ac.ir/journal/article-1-2841-en.html


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Volume 18, Issue 3 (10-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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