[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aim and Scope
Content Coverage
Ethics
Conflict of Interest
Copyrights & License
Price
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Current Issue
All Issues
Instruction to Authors::
Peer-Review::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 18, Issue 2 (6-2016) ::
J Gorgan Univ Med Sci 2016, 18(2): 120-126 Back to browse issues page
Atypical Yersinia virulence markers isolated from children with diarrhea
Soltan Dallal MM 1, Vafaei Z2 , Rahimi Foroushani A3 , Haghi Ashtiani MT4 , Sharifi Yazdi MK5 , Kavan M2 , Bakhtiari R2 , Nikmanesh B6
1- Professor, Division of Medical Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Professor, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran , msoltandallal@gmail.com
2- M.Sc in Medical Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Professor, Department of Epidemiology and Biostatics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4- Professor, Laboratory Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran
5- Professor, Zoonosis Research Centre, Tehran University of Medical Sciences, Tehran, Iran. Professor, Department of Medical Laboratory Sciences, School of Para Medicine, Tehran University of Medical Sciences, Tehran, Iran
6- Assistant Professor, Department of Medical Laboratory Sciences, School of Para Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (10992 Views)

Background and Objective: Yersinia is a gram-negative bacillus that cause diarrhea through consumption of contaminated food and water.  This study was performed to identify the atypical Yersinia virulence markers isolated from children with diarrhea.

Methods: This descriptive cross -sectional study was done on 384 fecal samples of 0- 14 years old children admitted at children medical center from August 2011 to August of 2012. Fecal samples, for the enrichment, after 21 days of incubation in alkaline buffer with pH=7.2 at 4degree C, on days 7, 14 and 21 samples were cultured on CIN agar and Mac agar and then confirm the differentiation atypical Yersinia from other typical Yersinia species from fermentation of different sugars. Isolates were tested for marker of virulence including calcium dependence, auto agglutination, Congo red uptake and binding of crystal violet.

Results: Out of 384 stool samples, 4 (1.04%) were infected with Yersinia (Yersinia frederikseni, Yersinia kristensenii and Yersinia enterocolitica). Out of these three, only two samples in association was positive with virulence markers.

Conclusion: Phenotypic markers can be used to study the properties of phenotypic strains of Yersinia.
Keywords: Yersinia enterocolitica, Atypical Yersinia, Virulence marker, Diarrhea
Full-Text [PDF 236 kb] [English Abstract]   (16191 Downloads) |   |   Abstract (HTML)  (557 Views)  
Type of Study: Original Articles | Subject: Microbiology
References
1. Velayati AA, Ghazi Saidi K, Travati MR. [A study of Salmonella, Shigella and Enteropathogenic Escherichia coli serotypes in acute gastroenteritis children under the age of five]. Med J Islam Repub Iran. 1987; 1(1): 22-31. [Article in Persian]
2. Torres ME, Pirez MC, Schelotto F, Varela G, Parodi V, Allende F, et al. Etiology of childrens diarrhea in Montevideo, Uruguay: associated pathogenic and unusual isolates. J Clin Microbiol. 2001 Jun; 39(6): 2134-9.
3. Bercovier H, Mollaret H. Genus XIV: Yersinia. In: Krieg NR (ed.), Bergey's Manual of Systematic Bacteriology. 1st ed. Baltimore: Williams &Wilkins. 1984; pp: 498-506.
4. Gierczyński R. Evaluation of the usefulness of selected virulence markers for the identification of virulent Yersinia enterocolitica strains. I. Phenotypic markders associated with Plasmid pYV. Med Dosw Mikrobiol. 2000; 52(1): 25-34.
5. Nathues C, Grüning P, Fruth A, Verspohl J, Blaha T, Kreienbrock L, Merle R. Campylobacter spp., Yersinia enterocolitica, and Salmonella enterica and their simultaneous occurrence in German fattening pig herds and their environment. J Food Prot. 2013 Oct; 76(10): 1704-11. doi:10.4315/0362-028X.JFP-13-076
6. Pal M. Zoonoses. 2nd ed. India, Jaipur: Satyam Publishers. 2007; pp: 151-2.
7. Bernardino-Varo L, Quiñones-Ramírez EI, Fernández FJ, Vázquez-Salinas C. Prevalence of Yersinia enterocolitica in raw cow's milk collected from stables of Mexico City. J Food Prot. 2013 Apr; 76(4): 694-8. doi:10.4315/0362-028X.JFP-12-325
8. Jay JM. Modern Food Microbiology. 6th ed. Maryland: Aspen Publishers, Inc. 2000; pp: 549-63.
9. Rosner BM, Stark K, Höhle M, Werber D. Risk factors for sporadic Yersinia enterocolitica infections, Germany 2009-2010. Epidemiol Infect. 2012 Oct; 140(10): 1738-47. doi:10.1017/S0950268811002664
10. Shayegani M, DeForge I, McGlynn DM, Root T. Characteristics of Yersinia enterocolitica and related species isolated from human, animal, and environmental sources. J Clin Microbiol. 1981 Sep; 14(3): 304-12.
11. Loftus CG, Harewood GC, Cockerill FR 3rd, Murray JA. Clinical features of patients with novel Yersinia species. Dig Dis Sci. 2002 Dec; 47(12): 2805-10.
12. Noble MA, Barteluk RL, Freeman HJ, Subramaniam R, Hudson JB. Clinical significance of virulence-related assay of Yersinia species. J Clin Microbiol. 1987 May; 25(5): 802-7.
13. Prpic JK, Robins-Browne RM, Davey RB. In vitro assessment of virulence in Yersinia enterocolitica and related species. J Clin Microbiol. 1985 Jul; 22(1): 105-10.
14. Falcão JP, Falcão DP, Pitondo-Silva A, Malaspina AC, Brocchi M. Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food originsisolated between 1968 and 2000 in Brazil. J Med Microbiol. 2006 Nov; 55(Pt 11): 1539-48.
15. Soltan Dallal MM, Vafaei Z, Haghi Ashtiani MT, Nikmanesh B, Rahimi Foroushani A. [Antibiotic susceptibility of Yersinia spp isolated from children with diarrhea]. J Gorgan Uni Med Sci. 2015; 17(1): 114-18. [Article in Persian]
16. Marks MI, Pai CH, Lafleur L, Lackman L, Hammerberg O. Yersinia enterocolitica gastroenteritis: a prospective study of clinical, bacteriologic, and epidemiologic features. J Pediatr. 1980 Jan; 96(1): 26-31.
17. Soltan-Dallal MM, Moezardalan K. Frequency of Yersinia species infection in paediatric acute diarrhoea in Tehran. East Mediterr Health J. 2004 Jan-Mar; 10(1-2): 152-8.
18. Ray SM, Ahuja SD, Blake PA, Farley MM, Samuel M, Fiorentino T, et al. Population-based surveillance for Yersinia enterocolitica infections in FoodNet sites, 1996-1999: higher risk ofdisease in infants and minority populations. Clin Infect Dis. 2004 Apr; 38 (Suppl 3): S181-9.
19. Lee LA, Taylor J, Carter GP, Quinn B, Farmer JJ, Tauxe RV. Y.enterocolitica 0:3 an emerging cause of pediatric gastroenteritis in the United States. J Infec Dis. 1991; 163(3): 660-3.
20. Laukkanen-Ninios R, Fredriksson-Ahomaa M, Maijala R, Korkeala H. High prevalence of pathogenic Yersinia enterocolitica in pig cheeks. Food Microbiol. 2014 Oct; 43: 50-2. doi:10.1016/j.fm.2014.04.016
21. Tauxe RV, Vandepitte J, Wauters G, Martin SM, Goossens V, De Mol P, et al. Yersinia enterocolitica infections and pork: the missing link. Lancet. 1987 May; 1(8542): 1129-32.
22. MacDonald E, Heier BT, Nygård K, Stalheim T, Cudjoe KS, Skjerdal T, et al. Yersinia enterocolitica outbreak associated with ready-to-eat salad mix, Norway, 2011. Emerg Infect Dis. 2012 Sep; 18(9): 1496-9. doi:10.3201/eid1809.120087
23. Hoogkamp-Korstanje JA, Stolk-Engelaar VM. Yersinia enterocolitica infection in children. Pediatr Infect Dis J. 1995 Sep; 14(9): 771-5.
24. Acha NP, Szyfres B. Zoonoses and Communicable Disease Common to Man and Animal. 3rd ed. Washington, DC: Pan American Health Organization. 2003; pp: 122-32.
25. Soltan Dallal MM, Salmanian H. [The prevalence rate of Yersinia enterocolitica in surface water and drinking area kan (Tehran)]. Iran J Infect Dis Trop Med. 1996; 1(2): 44-50. [Article in Persian]
26. Sulakvelidze A. Yersiniae other than Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis: the ignored species. Microbes Infect. 2000 Apr; 2(5): 497-513.
27. Zheng H, Sun Y, Mao Z, Jiang B. Investigation of virulence genes in clinical isolates of Yersinia enterocolitica. FEMS Immunol Med Microbiol. 2008 Aug; 53(3): 368-74. doi:10.1111/j.1574-695X.2008.00436.x
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Soltan Dallal MM, Vafaei Z, Rahimi Foroushani A, Haghi Ashtiani MT, Sharifi Yazdi MK, Kavan M, et al . Atypical Yersinia virulence markers isolated from children with diarrhea. J Gorgan Univ Med Sci 2016; 18 (2) :120-126
URL: http://goums.ac.ir/journal/article-1-2772-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 2 (6-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.04 seconds with 36 queries by YEKTAWEB 4645