:: Volume 19, Issue 1 (3-2017) ::
J Gorgan Univ Med Sci 2017, 19(1): 89-95 Back to browse issues page
Comparison of hypericin content, antioxidant, antimicrobial and cytotoxic activities of Hypericum perforatum L. from three geographic regions of Iran
M Akhbari * 1, M Ebrahimian2
1- Assistant Professor, Department of Phytochemistry, Essential Oil Research Institute, University of Kashan, Kashan, Iran , m_akhbari@kashanu.ac.ir
2- M.Sc in Organic Chemistry, Department of Chemistry, Shahr-e-Ray Branch, Islamic Azad University, Tehran, Iran
Abstract:   (10304 Views)

Background and Objective: Hypericin is found in different species of Hypericum genus, as a main compound with antimicrobial, antiviral, nonspecific kinas inhibition and dopamin β-hydroxilase inhibitoring effects. This study was done to compare the hypericin content, antioxidant, antimicrobial and cytotoxic activities of Hypericum perforatum L. from three geographic regions of Iran.

Methods: In this descriptive study, Hypericin content of aerial parts of H. perforatum L. was assessed using UV-Vis spectrometric method. Antioxidant activity was measured using DPPH and β-carotene bleaching assay. Cytotoxicity was evaluated via brine shrimp lethality assay. Antimicrobial activity was determined using inhibition zone diameter evaluation via disc diffusion method and measuring minimum inhibitory concentration (MIC) value.

Results: Hypericin content of aerial parts of H. perforatum L. from Qom, Golestan and Kurdestan provinces were 673, 1223 and 1568 ppm, respectively. Antioxidant and cytotoxic activities in samples from Kurdestan was more than samples from Qom and Golestan. Antimicrobial activity, as far as the number of sensitive microorganisms was evaluated. In this way the order of Golestan>Kurdestan>Qom was exhibited, however the extract of the plant from Kurdestan had the highest activity for two staphylococcus species with the inhibition zone diameter of 17 and 19 mm for S. aureus and S.epidermidis, respectively and MIC value of 250 µg/mL.

Conclusion: Hypericin content was more from samples of Kurdistan province with cold climate. Antimicrobial, antioxidant and cytotoxic activities of aerial parts of all samples were high. There is a relationship between hypericin content of aerial parts of H. perforatum L. and biological activities.
Keywords: Hypericum perforatum L., Hypericin, Antioxidant activity, Cytotoxic activity, Antimicrobial activity
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Type of Study: Original Articles | Subject: Microbiology
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