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:: Volume 20, Issue 3 (10-2018) ::
J Gorgan Univ Med Sci 2018, 20(3): 70-75 Back to browse issues page
Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen
Sasan Badi1 , Hami Kaboosi * 2, Hossian Abbaspoor3
1- M.Sc in Genetic Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2- Assistant Professor, Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. hkaboosi@gmail.com , hkaboosi@gmail.com
3- Associate Professor, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Abstract:   (8051 Views)
Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples.
Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated.
Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms.
Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples.
Keywords: Digestive diseases, Helicobacter pylori, vacA gene alleles
Full-Text [PDF 283 kb] [English Abstract]   (16972 Downloads) |   |   Abstract (HTML)  (445 Views)  
Type of Study: Original Articles | Subject: Microbiology
* Corresponding Author Address: Assistant Professor, Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. hkaboosi@gmail.com
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Badi S, Kaboosi H, Abbaspoor H. Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen . J Gorgan Univ Med Sci 2018; 20 (3) :70-75
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Volume 20, Issue 3 (10-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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