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:: Volume 20, Issue 3 (10-2018) ::
J Gorgan Univ Med Sci 2018, 20(3): 90-98 Back to browse issues page
Experimental evaluation of biocompatible pegylated phospholipid formulation containing Thyme essential oils and evaluation of anti-fungal activity of nanoscale system
Samira Naderinezhad1 , Shohre Babasafari2 , Fateme Haghiralsadat 3
1- M.Sc in Biotechnology, Department of Biotechnology and Pharmaceutical Engineering, School of Engineering, University of Tehran, Tehran, Iran
2- M.Sc in Biochemistry, Department of Biology, Payame Noor University, Yazd, Taft, Iran
3- Ph.D in Nanobiotechnology, Assistant Professor, Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. fhaghirosadat@ut.ac.ir , fhaghirosadat@ut.ac.ir
Abstract:   (9176 Views)
Background and Objective: Thyme essential oil is strong antifungal, anti-parasitic and antibacterial due to presence phenolic thymol and carvacrol. Encapsulation is used in order to increase the solubility, protection against oxidation and evaporation and also to improve the effectiveness of essential oils. This study was performed in order to loading Thymus essential oil (Thymus daenensis Celak and Zataria multiflora species) into liposomal vesicles and evaluation of antifungal activity of Zataria multiflora specie encapsulated with nano-systems.
Methods: In this descriptive – laboratory study, liposomes containing Thymus essential oil- were prepared using thin film hydration method. After size reduction, nano-particle was characterized in term of morphology, size, zeta potential and chemical interactions. Effect of phospholipids types, cholesterol content and species of Thymus were investigated on encapsulation efficiency. Finally, the antifungal activity of essential oil of Zataria multiflora specie loaded liposome was evaluated the minimum fungicidal concentration, minimum inhibitory concentration and zone of inhibition against Trichophyton mentagrophytes.
Results: Thymus essential oil loaded into liposome with over 80% efficiency. Optimal formulation contained of 10% cholesterol, 90% soybean phosphatidylcholine phospholipid with 3% of polyethylene glycol and also Thymus essential oil with concentration of 0.5 mg/ml. Nanoparticles were anionic with spherical shape and size less than 100 nm. There was no chemical interaction between liposomes and essential oil. Prepared formulation was chemically stable and the essential oil had not retained during encapsulation. Medicinal-nano system of Zataria multiflora specie was effective in inhibition of the growth of Trichophyton mentagrophytes.
Conclusion: The preparation of optimal liposomal formulation containing Thymus essential oil is affected by the type and amount of phospholipid, and it was completely independent of the species of Thymus. Also, Encapsulation increases the anti-fungal activity of essential oil of Zataria multiflora.
Keywords: Thyme, Liposome, Anti-fungal activity, Trichophyton mentagrophytes, Sustained-release, Pegylated
Full-Text [PDF 355 kb] [English Abstract]   (12956 Downloads) |   |   Abstract (HTML)  (380 Views)  
Type of Study: Original Articles | Subject: Medical Biotecnology
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Naderinezhad S, Babasafari S, Haghiralsadat F. Experimental evaluation of biocompatible pegylated phospholipid formulation containing Thyme essential oils and evaluation of anti-fungal activity of nanoscale system. J Gorgan Univ Med Sci 2018; 20 (3) :90-98
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Volume 20, Issue 3 (10-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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