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:: Volume 27, Issue 4 (12-2025) ::
J Gorgan Univ Med Sci 2025, 27(4): 83-91 Back to browse issues page
Protective Effects of Lycopene and Coenzyme Q10 Against 5-Fluorouracil-Induced Genotoxicity in SW480 (Colorectal Cancer) and Bone Marrow Mesenchymal Stem Cells Cell Lines: A Comet Assay Study
Elahe Gharehkhani1 , Sajedeh Zibayi2 , Mahboube Rahmati Kukandeh3 , Ramin Ataee4 , Mohammad Shokrzadeh *5
1- Ph.D in Toxicology, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
2- Pharmacy Student, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Ramsar, Iran.
3- Ph.D in Toxicology, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran., Mazandaran, Sari
4- Associate Professor of Pharmacology, Medicinal Plants Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
5- Professor, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran. Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran. , mshokrzadeh@mazums.ac.ir
Abstract:   (552 Views)
Background and Objective: 5-fluorouracil (5-FU) is a widely used chemotherapeutic agent for colorectal cancer treatment; however, its genotoxicity can lead to deoxyribonucleic acid (DNA) damage in healthy cells. Lycopene and Coenzyme Q10 are natural antioxidants capable of exerting protective effects against oxidative damage. This study was conducted to determine the protective effect of lycopene combined with Q10 against 5-FU-induced genotoxicity in colorectal cancer (SW480) and bone marrow mesenchymal stem cells (MSCs) cell lines using the Comet assay.
Methods: This descriptive-analytical study was conducted on SW480 and MSCs cell lines obtained from the Iranian Genetic Resources Cell Bank at the Cell Culture Laboratory of the Faculty of Pharmacy in 2023. The SW480 and MSCs cell lines were cultured at a density of 104 and exposed to a single dose of 5-FU (1 µM) along with various concentrations of lycopene and Q10 (0, 10, 20, and 30 µM). For each cell line, seven groups were defined: A control group (without treatment); a 5-FU group at optimum concentration (1 µM); groups receiving Q10 at 10, 20, and 30 µM plus lycopene at 10, 20, and 30 µM, respectively, comined with receiving 5-FU at optimum concentration (1 µM); a group receiving Q10 alone (30 µM); and a group receiving lycopene alone (30 µM). Cytotoxicity was evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, and DNA damage was assessed via the Comet assay.
Results: 5-FU caused a significant decrease in cell viability and a significant increase in DNA damage (P<0.05). Lycopene and Q10 alone did not exhibit significant cytotoxicity. The combination of lycopene and Q10 with 5-FU culminated in increased cell viability and decreased DNA damage compared to the group treated with 5-FU alone.
Conclusion: Lycopene and Q10 demonstrated significant protective effects against 5-FU-induced genotoxicity in both SW480 and MSCs cell lines.
 
Keywords: Fluorouracil [MeSH], Lycopene [MeSH], Coenzyme Q10 [MeSH], Mutagenicity Tests [MeSH], Comet Assay [MeSH]
Article ID: Vol27-39
Full-Text [PDF 692 kb]   (69 Downloads) |   |   Abstract (HTML)  (4 Views)  
Type of Study: Original Articles | Subject: Pharmacology
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Gharehkhani E, Zibayi S, Rahmati Kukandeh M, Ataee R, Shokrzadeh M. Protective Effects of Lycopene and Coenzyme Q10 Against 5-Fluorouracil-Induced Genotoxicity in SW480 (Colorectal Cancer) and Bone Marrow Mesenchymal Stem Cells Cell Lines: A Comet Assay Study. J Gorgan Univ Med Sci 2025; 27 (4) :83-91
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Volume 27, Issue 4 (12-2025) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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