|
Effect of a High and Medium Intensity Interval Training Period with Liposomal Alpha-Lipoic Acid Supplementation on the Expression of Sema3A Protein in the Soleus Muscle of the Induced Diabetic Rats
|
Seyedeh Fatemeh Fatemi1    , Seyed Abdollah Hashemvarzi * 2, Minoo Dadban Shahamat3 , Amin Farzaneh Hessari4 |
1- Ph.D Candidate in Exercise Physiology, Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
2- Assistant Professor, Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran. , hashemvarzi_tkd@yahoo.com
3- Assistant Professor of Sport Physiology, Faculty of Human Sciences, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran.
4- Assistant Professor, Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran. |
|
|
Abstract: (2174 Views) |
Background and Objective: Diabetes leads to impaired blood supply to the peripheral nerves. Sema3A (Semaphorin 3A) is a denervated protein that increases in response to hyperglycemia caused by diabetes. Exercise and alpha-lipoic acid (ALA) supplements can protect against diabetes-induced denervation. This study was done to evaluate the effect of high and moderate-intensity interval training with alpha-lipoic acid supplementation on the expression of Sema3A protein in the soleus muscle of Induced diabetic rats.
Methods: In this experimental study, thirty-five male Wistar rats (weight range: 190-220 g, 6-8 weeks old) were randomly allocated into seven groups of five: healthy control, diabetic, diabetic supplement (S), diabetic high-intensity training (HIT), diabetic moderate-intensity training (MIT), diabetic high intensity+supplement (HIT+S), and diabetic moderate intensity training+supplement (MIT+S). Diabetes was induced by injection of streptozotocin (50 mg/kg/bw). The HIT and MIT protocols were performed five days a week for six weeks. ALA was administered orally at 20 mg/kg daily by gavage. Immunohistochemistry was used to evaluate the expression of Sema3A protein in the soleus muscle. Serum insulin was measured by the ELISA method.
Results: Diabetes leads to increased level of glucose, Sema3A, and a significant decrease in insulin in the soleus muscle compared to healthy (P<0.05). HIT and MIT in combination with ALA, significantly showed lower expression of Sema3A Protein than in the diabetic group (P<0.05).
Conclusion: Although HIT and MIT can reduce the expression of Sema3A protein in the soleus muscle of diabetic rats, combining alpha-lipoic acid supplementation with exercise training is more effective in reducing the amount of denervation. |
|
Keywords: Alpha-lipoic acid [MeSH], Interval Training [MeSH], Semaphorin [MeSH], Diabetes Mellitus [MeSH]
Article ID: Vol25-17 |
|
|
Full-Text [PDF 1038 kb]
(4508 Downloads)
|
|
Type of Study: Original Articles |
Subject:
Exercise Physiology
|
|
|
|
|
|
|
| References |
1. Handsaker JC, Brown SJ, Bowling FL, Maganaris CN, Boulton AJ, Reeves ND. Resistance exercise training increases lower limb speed of strength generation during stair ascent and descent in people with diabetic peripheral neuropathy. Diabet Med. 2016 Jan; 33(1): 97-104. doi: 10.1111/dme.12841. [ DOI] [ PubMed] 2. Huang Z, Fang Q, Ma W, Zhang Q, Qiu J, Gu X, Yang H, Sun H. Skeletal Muscle Atrophy Was Alleviated by Salidroside Through Suppressing Oxidative Stress and Inflammation During Denervation. Front Pharmacol. 2019 Sep; 10: 997. doi: 10.3389/fphar.2019.00997. [ DOI] [ PubMed] 3. Mohamed R, Ranganathan P, Jayakumar C, Nauta FL, Gansevoort RT, Weintraub NL, et al. Urinary semaphorin 3A correlates with diabetic proteinuria and mediates diabetic nephropathy and associated inflammation in mice. J Mol Med (Berl). 2014 Dec; 92(12): 1245-56. doi: 10.1007/s00109-014-1209-3. [ DOI] [ PubMed] 4. Dumitru A, Radu BM, Radu M, Cretoiu SM. Muscle Changes During Atrophy. Adv Exp Med Biol. 2018; 1088: 73-92. doi: 10.1007/978-981-13-1435-3_4. [ DOI] [ PubMed] 5. Alvarez-Suarez P, Gawor M, Prószyński TJ. Perisynaptic schwann cells - The multitasking cells at the developing neuromuscular junctions. Semin Cell Dev Biol. 2020 Aug; 104: 31-38. doi: 10.1016/j.semcdb.2020.02.011. [ DOI] [ PubMed] 6. Lu Q, Zhu L. The Role of Semaphorins in Metabolic Disorders. Int J Mol Sci. 2020 Aug; 21(16): 5641. doi: 10.3390/ijms21165641. [ DOI] [ PubMed] 7. Kocaoğlu S, Aktaş Ö, Zengi O, Tufan A, Güzey FK. Effects of alpha lipoic acid on motor function and antioxidant enzyme activity of nerve tissue after sciatic nerve crush injury in rats. Turk Neurosurg. 2017 Aug. doi: 10.5137/1019-5149.JTN.18585-16.1. Online ahead of print. [ DOI] [ PubMed] 8. Oyenihi AB, Ayeleso AO, Mukwevho E, Masola B. Antioxidant strategies in the management of diabetic neuropathy. Biomed Res Int. 2015; 2015: 515042. doi: 10.1155/2015/515042. [ DOI] [ PubMed] 9. Ajith TA. Alpha-lipoic acid: A possible pharmacological agent for treating dry eye disease and retinopathy in diabetes. Clin Exp Pharmacol Physiol. 2020 Dec; 47(12): 1883-90. doi: 10.1111/1440-1681.13373. [ DOI] [ PubMed] 10. Jeffrey S, Samraj PI, Raj BS. The Role of Alpha-lipoic Acid Supplementation in the Prevention of Diabetes Complications: A Comprehensive Review of Clinical Trials. Curr Diabetes Rev. 2021; 17(9): e011821190404. doi: 10.2174/1573399817666210118145550. [ DOI] [ PubMed] 11. Najafi R, Sharifi AM, Hosseini A. Protective effects of alpha lipoic acid on high glucose-induced neurotoxicity in PC12 cells. Metab Brain Dis. 2015 Jun; 30(3): 731-38. doi: 10.1007/s11011-014-9625-1. [ DOI] [ PubMed] 12. Khanevari T, Rohani H, Vakili J, Sari sarraf V. [Effect of High-Intensity Interval Training on Leptin, Adiponectin, and Leptin/Adiponectin Ratio in Overweight Adolescent Boys]. Yafte. 2021; 23(3): 43-56. doi: 10.32592/Yafteh.2021.23.3.5 [Article in Persian] [ View at Publisher] [ DOI] 13. Holmes CJ, Hastings MK. The Application of Exercise Training for Diabetic Peripheral Neuropathy. J Clin Med. 2021 Oct; 10(21): 5042. doi: 10.3390/jcm10215042. [ DOI] [ PubMed] 14. Lee Y, Kim JH, Hong Y, Lee SR, Chang KT, Hong Y. Prophylactic effects of swimming exercise on autophagy-induced muscle atrophy in diabetic rats. Lab Anim Res. 2012 Sep; 28(3): 171-79. doi: 10.5625/lar.2012.28.3.171. [ DOI] [ PubMed] 15. Shaban N, Kenno KA, Milne KJ. The effects of a 2 week modified high intensity interval training program on the homeostatic model of insulin resistance (HOMA-IR) in adults with type 2 diabetes. J Sports Med Phys Fitness. 2014 Apr; 54(2): 203-209. [ PubMed] 16. Tayebi SM, Siahkouhian M, Keshavarz M, Yousefi M. The Effects of High-Intensity Interval Training on Skeletal Muscle Morphological Changes and Denervation Gene Expression of Aged Rats. Montenegrin Journal of Sports Science and Medicine. 2019; 8(2): 39-45. doi: 10.26773/mjssm.190906. [ Link] [ DOI] 17. Mahdian H, Farzanegi P, Farzaneh-Hessari A. [The effect of combined therapy with resveratrol, and continuous and interval exercises on levels of apoptotic biomarkers in heart tissue of male rats with non-alcoholic fatty liver]. Feyz. 2018; 22(5): 469-77. [Article in Persian] [ View at Publisher] 18. Holmes A, Coppey LJ, Davidson EP, Yorek MA. Rat Models of Diet-Induced Obesity and High Fat/Low Dose Streptozotocin Type 2 Diabetes: Effect of Reversal of High Fat Diet Compared to Treatment with Enalapril or Menhaden Oil on Glucose Utilization and Neuropathic Endpoints. J Diabetes Res. 2015; 2015: 307285. doi: 10.1155/2015/307285. [ DOI] [ PubMed] 19. Dworacka M, Chukanova G, Iskakova S, Kurmambayev Y, Wesołowska A, Frycz BA, et al. New arguments for beneficial effects of alpha-lipoic acid on the cardiovascular system in the course of type 2 diabetes. Eur J Pharm Sci. 2018 May; 117: 41-47. doi: 10.1016/j.ejps.2018.02.009. [ DOI] [ PubMed] 20. Karimi N, Bohlooli S, Mazani M. Nanoliposomal formulation of Ecballium elaterium Extract: Cytotoxic Evaluation against Human Gastric Adenocarcinoma (AGS) Cell Line. Nanomed Res J. 2016; 1(1): 9-14. doi: 10.7508/nmrj.2016.01.002. [ View at Publisher] [ DOI] 21. Hafstad AD, Lund J, Hadler-Olsen E, Höper AC, Larsen TS, Aasum E. High- and moderate-intensity training normalizes ventricular function and mechanoenergetics in mice with diet-induced obesity. Diabetes. 2013 Jul; 62(7): 2287-94. doi: 10.2337/db12-1580. [ DOI] [ PubMed] 22. Nakanishi Y, Kang S, Kumanogoh A. Axon guidance molecules in immunometabolic diseases. Inflamm Regen. 2022 Jan; 42(1): 5. doi: 10.1186/s41232-021-00189-0. [ DOI] [ PubMed] 23. Fazelzadeh M, Afzalpour MI, Fallah Mohammadi Z. [The Effect of 4-Week Voluntary Wheel Running on Hippocampus Levels of Semaphorin 3B and Hydrogen peroxide and Apoptosis in Diabetic Rats]. Med J Tabriz Uni Med Sciences Health Services. 2020 June- July; 42(2): 215-21. doi: 10.34172/mj.2020.039 [Article in Persian] [ View at Publisher] [ DOI] 24. Morinaka A, Yamada M, Itofusa R, Funato Y, Yoshimura Y, Nakamura F, et al. Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse. Sci Signal. 2011 Apr; 4(170): ra26. doi: 10.1126/scisignal.2001127. [ DOI] [ PubMed] 25. Wang Q, Wang PP, Meng PP, Han C, Yue SW. Intensive training accelerates the recovery of motor functions following cerebral ischemia-reperfusion in MCAO rats. Eur Rev Med Pharmacol Sci. 2016 Sep; 20(18): 3839-52. [ PubMed] 26. van Praag H. Exercise and the brain: something to chew on. Trends Neurosci. 2009 May; 32(5): 283-90. doi: 10.1016/j.tins.2008.12.007. [ DOI] [ PubMed] 27. Ghadiri Hormati L, Aminaei M, Dakhili AB, Asadi shekaari M. [The Effect of High-Intensity Exercise Training on Gene Expression of Semaphorin 3A in Extensor Digitorum Longus Muscles of Aged C57bl/6 Mice]. JIUMS. 2017; 25(1): 92-102. doi: 10.29252/sjimu.25.1.92 [Article in Persian] [ View at Publisher] [ DOI] 28. Fatemi SF, Hashemvarzi SA, Farzaneh Hesari A. [Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats]. JSSU. 2021; 29(8): 3976-88. doi: 10.18502/ssu.v29i8.7659 [Article in Persian] [ View at Publisher] [ DOI] 29. de Wit J, Verhaagen J. Role of semaphorins in the adult nervous system. Prog Neurobiol. 2003 Oct; 71(2-3): 249-67. doi: 10.1016/j.pneurobio.2003.06.001. [ DOI] [ PubMed] 30. Rahmati M, Khazani A, Gharakhanlou R, Movaheddin M, Manaheji H. Chronic effects of moderate intensity endurance training on neuropathic pain symptoms in diabetic rats. Physiol Pharmacol. 2013; 16(4): 435-45. [ View at Publisher] 31. Menéndez L, Lastra A, Hidalgo A, Baamonde A. Unilateral hot plate test: a simple and sensitive method for detecting central and peripheral hyperalgesia in mice. J Neurosci Methods. 2002 Jan; 113(1): 91-97. doi: 10.1016/s0165-0270(01)00483-6. [ DOI] [ PubMed] 32. Hoffman MD, Shepanski MA, Mackenzie SP, Clifford PS. Experimentally induced pain perception is acutely reduced by aerobic exercise in people with chronic low back pain. J Rehabil Res Dev. 2005 Mar-Apr; 42(2): 183-90. doi: 10.1682/jrrd.2004.06.0065. [ DOI] [ PubMed] 33. Gupta S, Sherikar A, Upaganlawar A, Upasani C. Neuroprotective effects of α-Lipoic acid alone and in combination with ferulic acid in diabetic neuropathy induced rats. DYSONA - Life Science. 2020; 1(3): 102-12. doi: 10.30493/dls.2020.243982. [ Link] [ DOI] 34. Elbadawy AM, Abd Elmoniem RO, Elsayed AM. Alpha lipoic acid and diabetes mellitus: potential effects on peripheral neuropathy and different metabolic parameters. Alexandria Journal of Medicine. 2021; 57(1): 113-20. doi: 10.1080/20905068.2021.1907961. [ Link] [ DOI] 35. Didangelos T, Karlafti E, Kotzakioulafi E, Kontoninas Z, Margaritidis C, Giannoulaki P, et al. Efficacy and Safety of the Combination of Superoxide Dismutase, Alpha Lipoic Acid, Vitamin B12, and Carnitine for 12 Months in Patients with Diabetic Neuropathy. Nutrients. 2020 Oct; 12(11): 3254. doi: 10.3390/nu12113254. [ DOI] [ PubMed] 36. Qiu J, Fang Q, Xu T, Wu C, Xu L, Wang L, et al. Mechanistic Role of Reactive Oxygen Species and Therapeutic Potential of Antioxidants in Denervation- or Fasting-Induced Skeletal Muscle Atrophy. Front Physiol. 2018 Mar; 9: 215. doi: 10.3389/fphys.2018.00215. [ DOI] [ PubMed] 37. Rababa'h AM, Mardini AN, Alzoubi KH, Ababneh MA, Athamneh RY. The effect of cilostazol on hippocampal memory and oxidative stress biomarkers in rat model of diabetes mellitus. Brain Res. 2019 Jul; 1715: 182-87. doi: 10.1016/j.brainres.2019.03.025. [ DOI] [ PubMed] 38. Mendoza-Núñez VM, García-Martínez BI, Rosado-Pérez J, Santiago-Osorio E, Pedraza-Chaverri J, Hernández-Abad VJ. The Effect of 600 mg Alpha-lipoic Acid Supplementation on Oxidative Stress, Inflammation, and RAGE in Older Adults with Type 2 Diabetes Mellitus. Oxid Med Cell Longev. 2019 Jun; 2019: 3276958. doi: 10.1155/2019/3276958. [ DOI] [ PubMed] 39. El Midaoui A, de Champlain J. Prevention of hypertension, insulin resistance, and oxidative stress by alpha-lipoic acid. Hypertension. 2002 Feb;39(2):303-7. doi: 10.1161/hy0202.104345. [ DOI] [ PubMed] |
|
| Send email to the article author |
|
|
|
Fatemi S F, Hashemvarzi S A, Dadban Shahamat M, Farzaneh Hessari A. Effect of a High and Medium Intensity Interval Training Period with Liposomal Alpha-Lipoic Acid Supplementation on the Expression of Sema3A Protein in the Soleus Muscle of the Induced Diabetic Rats. J Gorgan Univ Med Sci 2023; 25 (2) :22-31 URL: http://goums.ac.ir/journal/article-1-4185-en.html
|
