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Effects of Alkaline Phosphatase, GATA, and BMP Expression on Three Populations of Cells: Spermatogonial Stem Cells, Embryonic Cells, and Embryonic Stem-Like Cells in Mice
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Emad Reza1    , Hossein Azizi * 2 |
1- M.Sc Student of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
2- * Corresponding Author, Ph.D in Cellular and Molecular Biology, Stem Cells, Associate Professor, Faculty of Biotechnology, Amol University of Spcial Modern Technologies, Amol, Iran. , h.azizi@ausmt.ac.ir |
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Abstract: (2748 Views) |
Background and Objective: Alkaline phosphatase, BMP, and GATA proteins are important factors in the process of spermatogenesis. This study aims to investigate the effect of alkaline phosphatase, GATA, and BMP expression on spermatogenic stem cells, embryonic cells, and embryonic stem-like cells (ES-like) in C57BL mice.
Methods: In this experimental study, spermatogonial stem cells were isolated from three heads of 4-week-old C57BL mice, and embryonic stem cells and ES-like cells were prepared. Alkaline phosphatase staining test was performed on spermatogenic stem cells, embryonic cells, and ES-like cells. The expression of BMP and GATA genes was analyzed using Fluidigm PCR. Protein-protein interaction networks were isolated and drawn using databases.
Results: Positive alkaline phosphatase expression in stem cells and negative expression in testicular Sertoli cells indicated the presence of this enzyme in pluripotent cells. The gene expression of BMP and GATA in spermatogonial stem cells (6.3 and 2.7, respectively), embryonic cells (3.2 and 4.4, respectively), and ES-like cells (8.5 and 2.5, respectively) was positive, but not statistically significant. Bioinformatics studies showed the regulatory role of these genes and their direct effect on alkaline phosphatase.
Conclusion: BMP and GATA genes, along with alkaline phosphatase enzymes, play a crucial role in controlling embryonic and spermatogonial stem cells, maintaining their pluripotency, and guiding them towards differentiated cells.
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Keywords: Embryonic Stem Cells [MeSH], Adult Germline Stem Cells [MeSH], Alkaline Phosphatase [MeSH], GATA Transcription Factors [MeSH], Bone Morphogenetic Protein Receptors [MeSH]
Article ID: Vol25-04 |
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Full-Text [PDF 787 kb]
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Type of Study: Original Articles |
Subject:
Stem Cells
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Reza E, Azizi H. Effects of Alkaline Phosphatase, GATA, and BMP Expression on Three Populations of Cells: Spermatogonial Stem Cells, Embryonic Cells, and Embryonic Stem-Like Cells in Mice. J Gorgan Univ Med Sci 2023; 25 (1) :29-37 URL: http://goums.ac.ir/journal/article-1-4165-en.html
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