|
Effects of Endurance Training and Propolis Against Oxidative and Myocardial Stress in Diabetic Ovariectomized Rats
|
Fatemeh Hasanzadeh Dolatabadi1    , Khosro Jalali Dehkordi * 2, Farzaneh Taghian3 , Seyed Ali Hoseini4 |
1- Ph.D Candidate in Physical Education and Sport Sciences, Department of Physical Education and Sport Sciences, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.
2- Assistant Professor, Department of Physical Education and Sport Sciences, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran. , khosrojalali@gmail.com
3- Associate Professor, Department of Physical Education and Sport Sciences, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.
4- Professor, Department of Physical Education and Sport Sciences, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran. |
|
|
Abstract: (2337 Views) |
Background and Objective: Considering the role of diet and the use of natural antioxidants in addition to exercise, the attention of researchers has been drawn to take further advantage of the effects of exercise to improve cellular metabolism. Despite the favorable role of exercise and antioxidants on heart health, the simultaneous effect of exercise and consumption of propolis (prepared by bees) is not yet known. This study aimed to investigate the effects of endurance training and propolis against oxidative and myocardial stress in diabetic ovariectomized rats.
Methods: This experimental study was conducted on 36 female Sprague Dawley rats aged 12-16 weeks and weighing 220-250 grams. Six rats were included in the healthy control group. Diabetes was induced in 30 overiectomized rats by intraperitoneal injection of streptozotocin (40 mg/kg). Then, the diabetic animals were divided into five groups of six including diabetic ovariectomized control, sham, propolis, endurance training, and endurance training + propolis. Rats in the training groups trained for eight weeks, five sessions per week, with 55-75% VO2 max. Moreover, propolis was administered 100 mg/kg/day by peritoneal injection. The level of Pro-oxidant-antioxidant balance (PAB) and expression of HSP72 and NF-κB genes were measured.
Results: HSP72 expression was significantly higher in the propolis, endurance training, and endurance training + propolis groups. The levels of NF-kB and malonaldehyde were significantly decreased in the ovariectomized diabetic rats (P<0.05). Expression of HSP72 in the endurance training and endurance training + propolis groups were significantly higher than in the propolis group. Moreover, NF-kB in the endurance training and endurance training + propolis groups were significantly lower than in the propolis group (P<0.05). The value of PAB in the propolis and endurance training + propolis groups were significantly lower than in the ovariectomized diabetic rats (P<0.05).
Conclusion: Exercise and consumption of propolis seem to synergistically improve the protective HSP72 pathway and reduce oxidative stress in heart tissue following estrogen dysfunction and diabetes. However, the interaction of the two depends on endurance training.
|
|
Keywords: Endurance Training [MeSH], Propolis [MeSH], Oxidative Stress [MeSH], HSP 72 [MeSH], Heart [MeSH], Ovariectomy [MeSH], Diabetes Mellitus [MeSH]
Article ID: Vol24-51 |
|
|
Full-Text [PDF 742 kb]
(7243 Downloads)
|
|
Type of Study: Original Articles |
Subject:
Exercise Physiology
|
|
|
|
|
|
|
| References |
1. Sinatora RV, Chagas EFB, Mattera FOP, Mellem LJ, Santos AROD, Pereira LP, et al. Relationship of Inflammatory Markers and Metabolic Syndrome in Postmenopausal Women. Metabolites. 2022 Jan; 12(1): 73. doi: 10.3390/metabo12010073 [ DOI] [ PubMed] 2. El Khoudary SR, Aggarwal B, Beckie TM, Hodis HN, Johnson AE, Langer RD, et al. Menopause Transition and Cardiovascular Disease Risk: Implications for Timing of Early Prevention: A Scientific Statement From the American Heart Association. Circulation. 2020 Dec; 142(25): e506-e532. doi: 10.1161/CIR.0000000000000912 [ DOI] [ PubMed] 3. Conti FF, Brito Jde O, Bernardes N, Dias Dda S, Sanches IC, Malfitano C, et al. Cardiovascular autonomic dysfunction and oxidative stress induced by fructose overload in an experimental model of hypertension and menopause. BMC Cardiovasc Disord. 2014 Dec; 14: 185. doi: 10.1186/1471-2261-14-185 [ DOI] [ PubMed] 4. Anklam CFV, Lissarassa YPS, Dos Santos AB, Costa-Beber LC, Sulzbacher LM, Goettems-Fiorin PB, et al. Oxidative and Cellular Stress Markers in Postmenopause Women with Diabetes: The Impact of Years of Menopause. J Diabetes Res. 2021 Sep; 2021: 3314871. doi: 10.1155/2021/3314871 [ DOI] [ PubMed] 5. Bourgonje AR, Abdulle AE, Al-Rawas AM, Al-Maqbali M, Al-Saleh M, Enriquez MB, et al. Systemic Oxidative Stress Is Increased in Postmenopausal Women and Independently Associates with Homocysteine Levels. Int J Mol Sci. 2020 Jan; 21(1): 314. doi: 10.3390/ijms21010314 [ DOI] [ PubMed] 6. Szyller J, Bil-Lula I. Heat Shock Proteins in Oxidative Stress and Ischemia/Reperfusion Injury and Benefits from Physical Exercises: A Review to the Current Knowledge. Oxid Med Cell Longev. 2021 Jan; 2021: 6678457. doi: 10.1155/2021/6678457 [ DOI] [ PubMed] 7. Salehi OR, Ghabezi S, Khajehlandi A, Mohammadi A. Interactive effect of aerobic training and estrogen consumption on serum levels of catalase and glutathione peroxidase enzymes in. Jorjani Biomed J. 2020; 8(2): 38-47. doi: 10.52547/jorjanibiomedj.8.2.38 [ View at Publisher] [ DOI] 8. Davari F, Alimanesh Z, Alimanesh Z, Salehi O, Hosseini SA. Effect of training and crocin supplementation on mitochondrial biogenesis and redox-sensitive transcription factors in liver tissue of type 2 diabetic rats. Arch Physiol Biochem. 2022 Oct; 128(5): 1215-20. doi: 10.1080/13813455.2020.1762663 [ DOI] [ PubMed] 9. Stefani GP, Capalonga L, da Silva LR, Heck TG, Frizzo MN, Sulzbacher LM, et al. Effects of aerobic and resistance exercise training associated with carnosine precursor supplementation on maximal strength and V̇O2max in rats with heart failure. Life Sci. 2021 Oct; 282: 119816. doi: 10.1016/j.lfs.2021.119816 [ DOI] [ PubMed] 10. Chicco AJ, Hydock DS, Schneider CM, Hayward R. Low-intensity exercise training during doxorubicin treatment protects against cardiotoxicity. J Appl Physiol (1985). 2006 Feb; 100(2): 519-27. doi: 10.1152/japplphysiol.00148.2005 [ DOI] [ PubMed] 11. Hosseini SA, Salehi O, Keikhosravi F, Hassanpour G, Ardakani HD, Farkhaie F, et al. Mental Health Benefits of Exercise and Genistein in Elderly Rats. Exp Aging Res. 2022 Jan-Feb; 48(1): 42-57. doi: 10.1080/0361073X.2021.1918473 [ DOI] [ PubMed] 12. Oršolić N, Landeka Jurčević I, Đikić D, Rogić D, Odeh D, Balta V, et al. Effect of Propolis on Diet-Induced Hyperlipidemia and Atherogenic Indices in Mice. Antioxidants (Basel). 2019 Jun; 8(6): 156. doi: 10.3390/antiox8060156 [ DOI] [ PubMed] 13. Hosseini SA, Salehi OR, Farzanegi P, Farkhaie F, Darvishpour AR, Roozegar S. Interactive Effects of Endurance Training and Royal Jelly Consumption on Motor Balance and Pain Threshold in Animal Model of the Alzheimer Disease. Arch Neurosci. 2020; 7(2): e91857. doi: 10.5812/ans.91857 [ DOI] [ PubMed] 14. Kitamura H. Effects of Propolis Extract and Propolis-Derived Compounds on Obesity and Diabetes: Knowledge from Cellular and Animal Models. Molecules. 2019 Dec; 24(23): 4394. doi: 10.3390/molecules24234394 [ DOI] [ PubMed] 15. Al-Hariri MT, Eldin TAG, Al-Harb MM. Protective effect and potential mechanisms of propolis on streptozotocin-induced diabetic rats. Journal of Taibah University Medical Sciences. 2016 Feb; 11(1): 7-12. doi: 10.1016/j.jtumed.2015.11.002 [ Link] [ DOI] 16. Usman UZ, Bakar ABA, Mohamed M. Propolis improves pregnancy outcomes and placental oxidative stress status in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2018 Dec; 18(1): 324. doi: 10.1186/s12906-018-2391-6 [ DOI] [ PubMed] 17. Kwon TD, Lee MW, Kim KH. The effect of exercise training and water extract from propolis intake on the antioxidant enzymes activity of skeletal muscle and liver in rat. J Exerc Nutrition Biochem. 2014 Mar; 18(1): 9-17. doi: 10.5717/jenb.2014.18.1.9 [ DOI] [ PubMed] 18. Barthem CS, Rossetti CL, Carvalho DP, da-Silva WS. Metformin ameliorates body mass gain and early metabolic changes in ovariectomized rats. Endocr Connect. 2019 Dec; 8(12): 1568-78. doi: 10.1530/EC-19-0470 [ DOI] [ PubMed] 19. Souza CS, de Sousa Oliveira BS, Viana GN, Correia TML, de Bragança AC, Canale D, et al. Preventive effect of exercise training on diabetic kidney disease in ovariectomized rats with type 1 diabetes. Exp Biol Med (Maywood). 2019 Jun; 244(9): 758-69. doi: 10.1177/1535370219843830 [ DOI] [ PubMed] 20. Pahlavani N, Sedaghat A, Bagheri Moghaddam A, Mazloumi Kiapey SS, Gholizadeh Navashenaq J, Jarahi L, et al. Effects of propolis and melatonin on oxidative stress, inflammation, and clinical status in patients with primary sepsis: Study protocol and review on previous studies. Clin Nutr ESPEN. 2019 Oct; 33: 125-31. doi: 10.1016/j.clnesp.2019.06.007 [ DOI] [ PubMed] 21. Kavazis AN, Smuder AJ, Min K, Tümer N, Powers SK. Short-term exercise training protects against doxorubicin-induced cardiac mitochondrial damage independent of HSP72. Am J Physiol Heart Circ Physiol. 2010 Nov; 299(5): H1515-24. doi: 10.1152/ajpheart.00585.2010 [ DOI] [ PubMed] 22. Costa-Pereira LV, Melo DS, Santos CS, Mendes BF, Esteves EA, Lacerda ACR, et al. Distinct beneficial effects of continuous vs accumulated exercise training on cardiovascular risk factors in Wistar rats. Scand J Med Sci Sports. 2017 Nov; 27(11): 1384-94. doi: 10.1111/sms.12737 [ DOI] [ PubMed] 23. Akin S, Naito H, Ogura Y, Ichinoseki-Sekine N, Kurosaka M, Kakigi R, et al. Short-term treadmill exercise in a cold environment does not induce adrenal Hsp72 and Hsp25 expression. J Physiol Sci. 2017 May; 67(3): 407-13. doi: 10.1007/s12576-016-0473-0 [ DOI] [ PubMed] 24. Gholitabar S, Roshan VD. Effect of treadmill exercise and Ferula gummosa on myocardial HSP72, vascular function, and antioxidant defenses in spontaneously hypertensive rats. Clin Exp Hypertens. 2013; 35(5): 347-54. doi: 10.3109/10641963.2012.732643 [ DOI] [ PubMed] 25. Zeng Z, Centner C, Gollhofer A, König D. Effects of Dietary Strategies on Exercise-Induced Oxidative Stress: A Narrative Review of Human Studies. Antioxidants (Basel). 2021 Mar; 10(4): 542. doi: 10.3390/antiox10040542 [ DOI] [ PubMed] 26. Wang Q, Sui X, Sui DJ, Yang P. Flavonoid Extract from Propolis Inhibits Cardiac Fibrosis Triggered by Myocardial Infarction through Upregulation of SIRT1. Evid Based Complement Alternat Med. 2018 Jun; 2018: 4957573. doi: 10.1155/2018/4957573 [ DOI] [ PubMed] 27. Sun L, Wang K, Xu X, Ge M, Chen Y, Hu F. Potential Protective Effects of Bioactive Constituents from Chinese Propolis against Acute Oxidative Stress Induced by Hydrogen Peroxide in Cardiac H9c2 Cells. Evid Based Complement Alternat Med. 2017; 2017: 7074147. doi: 10.1155/2017/7074147 [ DOI] [ PubMed] 28. Silva H, Francisco R, Saraiva A, Francisco S, Carrascosa C, Raposo A. The Cardiovascular Therapeutic Potential of Propolis-A Comprehensive Review. Biology (Basel). 2021 Jan; 10(1): 27. doi: 10.3390/biology10010027 [ DOI] [ PubMed] 29. Silva DB, Miranda AP, Silva DB, D'Angelo LR, Rosa BB, Soares EA, et al. Propolis and swimming in the prevention of atherogenesis and left ventricular hypertrophy in hypercholesterolemic mice. Braz J Biol. 2015 May; 75(2): 414-22. doi: 10.1590/1519-6984.15313 [ DOI] [ PubMed] 30. Soleimani D, Miryan M, Hadi V, Gholizadeh Navashenaq J, Moludi J, Sayedi SM, et al. Effect of propolis supplementation on athletic performance, body composition, inflammation, and oxidative stress following intense exercise: A triple-blind randomized clinical trial. Food Sci Nutr. 2021 May; 9(7): 3631-40. doi: 10.1002/fsn3.2319 [ DOI] [ PubMed] |
|
| Send email to the article author |
|
|
|
Hasanzadeh Dolatabadi F, Jalali Dehkordi K, Taghian F, Hoseini S A. Effects of Endurance Training and Propolis Against Oxidative and Myocardial Stress in Diabetic Ovariectomized Rats. J Gorgan Univ Med Sci 2022; 24 (4) :51-58 URL: http://goums.ac.ir/journal/article-1-4136-en.html
|
