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:: Volume 23, Issue 4 (12-2021) ::
J Gorgan Univ Med Sci 2021, 23(4): 40-46 Back to browse issues page
Identification of SARS-CoV-2 Genome in the Blood Samples of COVID-19 Patients
Bahman Aghcheli1 , Abdolvahab Moradi2 , Alijan Tabarraei3 , Hamed Naziri4 , Mohammad Reza Kalani5 , Alireza Tahamtan * 6
1- Ph.D Candidate in Medical Virology, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
2- Professor of Medical Virology, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
3- Professor of Medical Virology, Infectious Diseases Research Center, School of Medicine, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran.
4- Assistant Professor of Medical Virology, Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
5- Assistant Professor of Molecular Medicine, Department of Molecular Medicine, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
6- Assistant Professor of Medical Virology, Infectious Diseases Research Center, School of Medicine, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran. , alireza.tmn@gmail.com
Abstract:   (5192 Views)
Background and Objective: Since the onset of the COVID-19 (Corona Virus Disease 2019) pandemic, several challenges have been proposed to the disease and the causing viral agent. Accurate and rapid diagnosis of the virus is essential to control the spread and progression of the disease. Choosing a suitable sample in different phases of the disease will reduce the false-negative results. This study was performed to identify the SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) genome in the blood samples of COVID-19 patients.
Methods: This descriptive-analytical study was performed by census method on 100 whole blood samples of patients (50 recovery and 50 deceased) with a definitive diagnosis of COVID-19 (positive Real-Time RT-PCR test of nasopharyngeal swab samples) admitted to Shahid Sayyad Shirazi educational and medical center in Gorgan during 2020-21. Clinical and laboratory findings were compared in the two groups of patients. The viral nucleic acid was extracted from the whole blood samples of the patients, and the presence of the virus genome was investigated using primer and probes via the Real-Time RT-PCR method.
Results: The age of the recovered patients (49.06±15.1 years) was significantly was lower than deceased patients (58.3±12.4 years) (P<0.05). Clinical symptoms including cough, shortness of breath, sputum secretion, and vomiting in deceased patients were significantly more than recovery group (P<0.05). The lymphocytes count and platelet level in the deceased group were lower than in the recovered group. Level of lactate dehydrogenase (LDH) was higher in the deceased group in compare to recovered group (P<0.05). The virus genome identified in the blood samples of 7 patients (3 recovered and 4 deceased), which had no significant relationship with the outcome of the disease.
Conclusion: The use of blood samples for the diagnosis of COVID-19 is not appropriate.
Keywords: COVID-19 [MeSH], SARS-CoV-2 [MeSH], COVID-19 Nucleic Acid Testing [MeSH]
Article ID: Vol23-52
Full-Text [PDF 534 kb]   (14826 Downloads)    
Type of Study: Original Articles | Subject: Virology
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Aghcheli B, Moradi A, Tabarraei A, Naziri H, Kalani M R, Tahamtan A. Identification of SARS-CoV-2 Genome in the Blood Samples of COVID-19 Patients. J Gorgan Univ Med Sci 2021; 23 (4) :40-46
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Volume 23, Issue 4 (12-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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