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Biotyping of Yersiniaenterocolitica Isolates from Children with Diarrhea and Chicken Meat in Tehran, Iran (2016-17)
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Mohammad Mehdi Soltan Dallal *    1, Mohammad Kazem Sharifi Yazdi2 , Alireza Monadi Sefidan3 , Gholamreza Hassanpour4 , Sara Sharifi Yazdi5 , Shabnam Haghighat Khajavi6 , Saeed Vahedi7 , Seyedeh Masoomeh Abrichamchian Langaroudi8 , Mahdieh Pourmoradian9 , Hedroosha Molla Agha Mirzaei10 |
1- Professor, Food Microbiology Research Center /Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran, Iran. , msoltandallal@gmail.com
2- Professor, Zoonosis Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
3- Associate Professor, Department of Laboratory Sciences, School of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran.
4- Associate Professor, Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran.
5- Medical Student, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
6- Assistant Professor, Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
7- Academic Instructor, Operating Room Department, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.
8- M.Sc in Microbiology, Pathobiology Laboratory Center, Tehran. Iran.
9- Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
10- M.Sc in Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. |
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Abstract: (3932 Views) |
Background and Objective: Yersinia is water and foodborne organism that cause human gastroenteritis. This study was done to evaluate the frequency of Yersinia species isolated from children diarrheal samples and chicken meat in Tehran, Iran.
Methods: In this descriptive study 250 sample of diarrhea of children referred to the Children's Medical Center, Tehran, Iran and 250 samples of chicken were collected and examined for Yersinia infection during July 2016 to March 2017. Isolation method was performed based on initial enrichment in phosphate buffer for 3 weeks in refrigerator (cooling in c4 +) and then using KOH as secondary enrichment and culture on CIN agar medium. Biotyping method was used to determine pathogenic strains.
Results: In this study, 5(2%) isolates from pediatric diarrhea samples and 20 isolates (8%) from chicken meat samples were obtained from Yersiniaenterocolitica. Biotyping of human Yersiniaenterocolitica isolates identified 3 cases of biotype 1A, one case of biotype 1B, one case of biotype 2 and from chicken meat isolates, 16 isolates belonged to biotype 1A and 4 isolates belonged to biotype 1B.
Conclusion: Presence of common pathogenic 1B and non-pathogenic 1A biotypes in pediatric diarrhea samples and chicken meat can indicate the cause of diarrhea in children.
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Keywords: Yersiniaenterocolitica [MeSH], Diarrhea [MeSH], Child [MeSH]
Article ID: Vol24-13 |
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Full-Text [PDF 537 kb]
(12257 Downloads)
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Type of Study: Original Articles |
Subject:
Microbiology
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Soltan Dallal M M, Sharifi Yazdi M K, Monadi Sefidan A, Hassanpour G, Sharifi Yazdi S, Haghighat Khajavi S, et al . Biotyping of Yersiniaenterocolitica Isolates from Children with Diarrhea and Chicken Meat in Tehran, Iran (2016-17). J Gorgan Univ Med Sci 2022; 24 (1) :94-99 URL: http://goums.ac.ir/journal/article-1-3996-en.html
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