|
Biotyping of Yersiniaenterocolitica Isolates from Children with Diarrhea and Chicken Meat in Tehran, Iran (2016-17)
|
Mohammad Mehdi Soltan Dallal *    1, Mohammad Kazem Sharifi Yazdi2 , Alireza Monadi Sefidan3 , Gholamreza Hassanpour4 , Sara Sharifi Yazdi5 , Shabnam Haghighat Khajavi6 , Saeed Vahedi7 , Seyedeh Masoomeh Abrichamchian Langaroudi8 , Mahdieh Pourmoradian9 , Hedroosha Molla Agha Mirzaei10 |
1- Professor, Food Microbiology Research Center /Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran, Iran. , msoltandallal@gmail.com
2- Professor, Zoonosis Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
3- Associate Professor, Department of Laboratory Sciences, School of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran.
4- Associate Professor, Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran.
5- Medical Student, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
6- Assistant Professor, Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
7- Academic Instructor, Operating Room Department, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.
8- M.Sc in Microbiology, Pathobiology Laboratory Center, Tehran. Iran.
9- Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
10- M.Sc in Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. |
|
|
Abstract: (3396 Views) |
Background and Objective: Yersinia is water and foodborne organism that cause human gastroenteritis. This study was done to evaluate the frequency of Yersinia species isolated from children diarrheal samples and chicken meat in Tehran, Iran.
Methods: In this descriptive study 250 sample of diarrhea of children referred to the Children's Medical Center, Tehran, Iran and 250 samples of chicken were collected and examined for Yersinia infection during July 2016 to March 2017. Isolation method was performed based on initial enrichment in phosphate buffer for 3 weeks in refrigerator (cooling in c4 +) and then using KOH as secondary enrichment and culture on CIN agar medium. Biotyping method was used to determine pathogenic strains.
Results: In this study, 5(2%) isolates from pediatric diarrhea samples and 20 isolates (8%) from chicken meat samples were obtained from Yersiniaenterocolitica. Biotyping of human Yersiniaenterocolitica isolates identified 3 cases of biotype 1A, one case of biotype 1B, one case of biotype 2 and from chicken meat isolates, 16 isolates belonged to biotype 1A and 4 isolates belonged to biotype 1B.
Conclusion: Presence of common pathogenic 1B and non-pathogenic 1A biotypes in pediatric diarrhea samples and chicken meat can indicate the cause of diarrhea in children.
|
|
Keywords: Yersiniaenterocolitica [MeSH], Diarrhea [MeSH], Child [MeSH]
Article ID: Vol24-13 |
|
|
Full-Text [PDF 537 kb]
(10214 Downloads)
|
|
Type of Study: Original Articles |
Subject:
Microbiology
|
|
|
|
|
|
|
| References |
1. Varela G, Batthyány L, Bianco MN, Pérez W, Pardo L, Algorta G, et al. Enteropathogens associated with acute diarrhea in children from households with high socioeconomic level in uruguay. Int J Microbiol. 2015; 2015: 592953. DOI: 10.1155/2015/592953 [ DOI] [ PubMed] 2. Tian L, Zhu X, Chen Z, Liu W, Li S, Yu W, et al. Characteristics of bacterial pathogens associated with acute diarrhea in children under 5 years of age: a hospital-based cross-sectional study. BMC Infect Dis. 2016 Jun; 16: 253. DOI: 10.1186/s12879-016-1603-2 [ DOI] [ PubMed] 3. Sabina Y, Rahman A, Ray RC, Montet D. Yersiniaenterocolitica: Mode of Transmission, Molecular Insights of Virulence, and Pathogenesis of Infection. J Pathog. 2011; 2011: 429069. DOI: 10.4061/2011/429069 [ DOI] [ PubMed] 4. Grahek-Ogden D, Schimmer B, Cudjoe KS, Nygård K, Kapperud G. Outbreak of Yersiniaenterocolitica serogroup O:9 infection and processed pork, Norway. Emerg Infect Dis. 2007 May; 13(5): 754-56. DOI: 10.3201/eid1305.061062 [ DOI] [ PubMed] 5. Imataki O, Uemura M, Matsumoto K, Ishibashi N. Yersinia pseudotuberculosis enterocolitis mimicking enteropathic γδ T-cell lymphoma with abnormal clonality. BMC Infect Dis. 2014 Jan; 14: 42. DOI: 10.1186/1471-2334-14-42 [ DOI] [ PubMed] 6. Soltan Dallal MM, Vafaei Z, Haghi Ashtiani MT, Nikmanesh B, Rahimi Foroushani A. [Antibiotic susceptibility of Yersinia spp isolated from children with diarrhea]. J Gorgan Univ Med Sci. 2015; 17(1): 114-18. [Article in Persian] [ View at Publisher] 7. Hamama A, el Marrakchi A, el Othmani F. Occurrence of Yersiniaenterocolitica in milk and dairy products in Morocco. Int J Food Microbiol. 1992 May; 16(1): 69-77. DOI: 10.1016/0168-1605(92)90127-o [ DOI] [ PubMed] 8. Soltan Dallal M, Izadpour F, Khalifeh Gholi M, Zeraati H, Bakhtiari R. [Prevalence of Yersinia spp. in red meat and chicken marketed in southern Tehran]. Sch Public Health Inst Public Health Res. 2006; 4(4): 49-56. [Article in Persian] [ View at Publisher] 9. Verbikova V, Borilova G, Babak V, Moravkova M. Prevalence, characterization and antimicrobial susceptibility of Yersiniaenterocolitica and other Yersinia species found in fruits and vegetables from the European :union:. Food Control. 2018 Mar; 85: 161-67. DOI: 10.1016/j.foodcont.2017.08.038 [ Article] [ DOI] 10. Kiani P, Bakhshi B, Soltan-Dallal MM, Najar-Peerayeh S. Heterogeneity of Highly Susceptible Yersiniaenterocolitica Isolates of Clinical and Environmental Origin: A 5-Year Survey from Iran (2011-2016). Microb Drug Resist. 2020 Jan; 26(1): 46-53. DOI: 10.1089/mdr.2018.0469 [ DOI] [ PubMed] 11. Ackers ML, Schoenfeld S, Markman J, Smith MG, Nicholson MA, DeWitt W, et al. An outbreak of Yersiniaenterocolitica O:8 infections associated with pasteurized milk. J Infect Dis. 2000 May; 181(5): 1834-37. DOI: 10.1086/315436 [ DOI] [ PubMed] 12. Soltan-Dallal MM, Tabarraie A, MoezArdalan K. Comparison of four methods for isolation of Yersiniaenterocolitica from raw and pasteurized milk from northern Iran. Int J Food Microbiol. 2004 Jul; 94(1): 87-91. DOI: 10.1016/j.ijfoodmicro.2003.10.017 [ DOI] [ PubMed] 13. Boghenbor KK, On SL, Kokotovic B, Baumgartner A, Wassenaar TM, Wittwer M, et al. Genotyping of human and porcine Yersiniaenterocolitica, Yersinia intermedia, and Yersinia bercovieri strains from Switzerland by amplified fragment length polymorphism analysis. Appl Environ Microbiol. 2006 Jun; 72(6): 4061-66. DOI: 10.1128/AEM.01996-05 [ DOI] [ PubMed] 14. Borjian S. [The common causes of bacterial diarrhea in children aged below 2 years in Borujen Hospital]. J Shahrekord Univ Med Sci. 1999; 1(1): 13-20. [Article in Persian] [ View at Publisher] 15. Kanan TA, Abdulla ZA. Isolation of Yersinia spp. from cases of diarrhoea in Iraqi infants and children. East Mediterr Health J. 2009 Mar-Apr; 15(2): 276-84 [ PubMed] 16. Jamali H, Paydar M, Radmehr B, Ismail S. Prevalence, characterization, and antimicrobial resistance of Yersinia species and Yersiniaenterocolitica isolated from raw milk in farm bulk tanks. Journal of Dairy Science. 2015 Feb; 98(2): 798-803. DOI: 10.3168/jds.2014-8853 [ Article] [ DOI] 17. Sharifi Yazdi MK, Soltan-Dallal MM, Zali MR, Avadisians S, Bakhtiari R. Incidence and antibiotic susceptibilities of Yersiniaenterocolitica and other Yersin species recovered from meat and chicken in Tehran, Iran. Afr J Microbiol Res. 2011; 5(18): 2649-53. [ Article] 18. Soltan-Dallal MM, Moezardalan K. Frequency of Yersinia species infection in paediatric acute diarrhoea in Tehran. East Mediterr Health J. 2004 Jan-Mar; 10(1-2): 152-58. [ PubMed] 19. Wauters G, Kandolo K, Janssens M. Revised biogrouping scheme of Yersiniaenterocolitica. Contrib Microbiol Immunol. 1987; 9: 14-21. [ PubMed] 20. Luca CM, Nemescu R, Teodor A, Fântânaru R, Petrovici CM, Dorobăţ C. [Etiological aspects of acute gastroenteritis--a ten-year review (1.01. 2001-31.12.2010)]. Rev Med Chir Soc Med Nat Iasi. 2011 Jul-Sep; 115(3): 712-17. [Article in Romanian] [ PubMed] 21. Soltan Dallal MM, Khorramizadeh MR, MoezArdalan K. Occurrence of enteropathogenic bacteria in children under 5 years with diarrhoea in south Tehran. East Mediterr Health J. 2006 Nov; 12(6): 792-97. [ PubMed] 22. Scallan E, Mahon BE, Hoekstra RM, Griffin PM. Estimates of illnesses, hospitalizations and deaths caused by major bacterial enteric pathogens in young children in the United States. Pediatr Infect Dis J. 2013 Mar; 32(3): 217-21. DOI: 10.1097/INF.0b013e31827ca763 [ DOI] [ PubMed] 23. Martínez PO, Fredriksson-Ahomaa M, Sokolova Y, Roasto M, Berzins A, Korkeala H. Prevalence of enteropathogenic Yersinia in Estonian, Latvian, and Russian (Leningrad region) pigs. Foodborne Pathog Dis. 2009 Jul-Aug; 6(6): 719-24. DOI: 10.1089/fpd.2008.0251 [ DOI] [ PubMed] 24. Tauxe RV, Vandepitte J, Wauters G, Martin SM, Goossens V, De Mol P, et al. Yersiniaenterocolitica infections and pork: the missing link. Lancet. 1987 May; 1(8542): 1129-32. DOI: 10.1016/s0140-6736(87)91683-7 [ DOI] [ PubMed] 25. Saberianpour S, Tajbakhsh E, Khamesipour F. Prevalaence of virulence genes and biotyping of Yersiniaenterocolitica of isolated from chicken meat in Shahrekord, Iran. Vidyabharati International Interdisciplinary Research Journal. 2014 Dec; 3(2): 71-76. [ View at Publisher] 26. Sirghani K, Zeinali T, Jamshidi A. Detection of Yersiniaenterocolitica in Retail Chicken Meat, Mashhad, Iran. J Pathog. 2018 Apr; 2018: 1286216. DOI: 10.1155/2018/1286216 [ DOI] [ PubMed] 27. Soltan Dallal MM, Vafaei Z, Rahimi Foroushani A, Haghi Ashtiani MT, Sharifi Yazdi MK, Kavan M, et al. [Atypical Yersinia virulence markers isolated from children with diarrhea]. J Gorgan Univ Med Sci. 2016; 18(2): 120-26. [Article in Persian] [ View at Publisher] 28. Haghighi L, Vahdat A. The first successful isolation and identification of Yersiniaenterocolitica in Iran. Pahlavi Med J. 1977 Jan; 8(1): 133-4. [ PubMed] |
|
| Send email to the article author |
|
|
|
Soltan Dallal M M, Sharifi Yazdi M K, Monadi Sefidan A, Hassanpour G, Sharifi Yazdi S, Haghighat Khajavi S, et al . Biotyping of Yersiniaenterocolitica Isolates from Children with Diarrhea and Chicken Meat in Tehran, Iran (2016-17). J Gorgan Univ Med Sci 2022; 24 (1) :94-99 URL: http://goums.ac.ir/journal/article-1-3996-en.html
|
