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:: Volume 24, Issue 1 (3-2022) ::
J Gorgan Univ Med Sci 2022, 24(1): 44-52 Back to browse issues page
The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats
Soren Valafar1 , Eidy Alijani 2, Fariba Aghaei3 , Mahsa Mohsenzadeh3
1- Ph.D Candidate in Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran.
2- Professor of Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran. , eidyalijani@yahoo.com
3- Assistant Professor of Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran.
Abstract:   (2721 Views)
Background and Objective: Type 1 diabetes (T1D) is highly prevalent in the group of autoimmune and inflammatory patients. Tumor necrosis factor-alpha (TNF-a) and interleukin-10 (IL-10) plays an important role in regulating complex interactions between pancreatic beta cells and immune cells in the development of T1D. This study was performed to determine the simultaneous effect of resistance training and endothelial progenitor cell injection on blood glucose levels and protein expression of proinflammatory factors TNF-a and IL-10 in muscle tissue of streptozotocin (STZ) induced diabetic male rats.
Methods: In this experimental study, 36 male Wistar rats weighing approximately 200±20 g and six weeks old were randomly divided into six groups. Induction of diabetes was performed by intraperitoneal injection of STZ at a dose of 45 mg/kg body weight. Groups included diabetes + stem cell injection + resistance training group, diabetes + resistance training group, diabetes + stem cell injection group, control diabetic group to control the passage of time, and healthy basal and diabetic groups for defaults. Exercises were performed for 17 sessions of resistance training, including climbing ladders with increasing weight three days a week in the same laboratory conditions. Endothelial progenitor cells were cultured by femoral bone marrow aspiration and culture and then injection into the tail vein. 68 hours after the last training session, blood glucose levels were assessed by ELISA and the expression of TNF-a and IL-10 protein in muscle tissue was assessed by Western blotting.
Results: Endothelial stem cell injection, resistance training and resistance training with the simultaneous injection of endothelial stem cells significantly increased the anti-inflammatory factor IL-10 in the skeletal muscle tissue of diabetic rats in compared to control group (P<0.05). Expression of the anti-inflammatory factor IL-10 in the skeletal muscle tissue was significantly increased in resistance training plus the simultaneous injection of endothelial stem cells group in compared to injection of stem cells and resistance training groups (P<0.05). Glucose concentration in the skeletal muscle tissue was significantly reduced in resistance training plus the simultaneous injection of endothelial stem cells group in compared to injection of stem cells and resistance training groups (P<0.05).
Conclusion: It seems that 17 sessions of resistance training reduces blood glucose level and improves inflammatory conditions in response to an increase in IL-10 and a decrease in TNF-a in a group of diabetic rats with resistance training and simultaneous injection of endothelial progenitor cells in diabetic male rats.
Keywords: Resistance Training [MeSH], Endothelial Progenitor Cells [MeSH], Type 1 Diabetes Mellitus [MeSH], Blood Glucose [MeSH], Tumor Necrosis Factor-alpha [MeSH], Interleukin-10 [MeSH]
Article ID: Vol24-06
Full-Text [PDF 849 kb]   (8252 Downloads)    
Type of Study: Experimental Study | Subject: Immunology
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Valafar S, Alijani E, Aghaei F, Mohsenzadeh M. The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats. J Gorgan Univ Med Sci 2022; 24 (1) :44-52
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Volume 24, Issue 1 (3-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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