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:: Volume 23, Issue 1 (3-2021) ::
J Gorgan Univ Med Sci 2021, 23(1): 31-37 Back to browse issues page
Comparison of the effect of lead acetate and Sodium Sulfide poisoning on Npm1 gene expression in adult male rats
Razieh Mottahedzadeh1 , Saeed Khatamsaz * 2, Mohammad Javad Mokhtari3
1- Ph.D in Biology – Physiology, Shiraz, Iran.
2- Associate Professor, Department of Biology, College of Science, Jeroft Branch, Islamic Azad University, Jeroft, Iran. , saeed1617@yahoo.com
3- Assistant Professor, Department of Biology, College of Science, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
Abstract:   (6163 Views)
Background and Objective: Evaluation of effective factors in the incidence of leukemia, especially pollutants, such as Sodium Sulfide and lead acetate, can contribute to the treatment of cancer and prevention of disease. Npm1 gene is a multiple Phosphoprotein that contains several action domains. Npm1 gene is encoded between nucleus and cytoplasm and performs several functions including protein ribosome transfer and control of centrosome proliferation. Npm1 mutations are transferred from the nucleus to the cytoplasm. This study was conducted to compare the effect of lead static poisoning and sodium sulfide on Npm1 gene expression in adult male rats.
Methods: In this experimental study, 48 adult male rats were allocated into 6 groups. The experimental groups include control, the first and second experimental groups were received sodium sulfide with doses of 300 mg/kg/bw and 600 mg/kg/bw, respectively. The third and fourth experimental groups were received lead acetate with doses of 30 mg/kg/bw and 60 mg/kg/bw, respectively. The fifth experimental groups were received maximum doses of sodium sulfide and lead acetate, respectively. Lead acetate and sodium sulfide were gavaged daily for 4 months. After that, blood was taken from the mice and RNA was extracted. Then, CDNA synthesis and Npm1 gene expression compared to Ywhaz gene were evaluated quantitatively using Real Time PCR.
Results: Npm1 gene expression reduced in groups were received sodium sulfide at doses of 300 and 600 mg/kg body weight. Npm1 expression increased in lead static groups with doses of 30 and 60 mg/kg body weight.
Conclusion: This study showed that by increasing the expression of Npm1 gene expression, Threshold Cycle value decreases.
Keywords: Acute Myeloid Leukemia [MeSH], Lead [MeSH], Nucleophosmin Gene [MeSH], Rats [MeSH]
Article ID: Vol23-04
Full-Text [PDF 832 kb]   (11877 Downloads)    
Type of Study: Original Articles | Subject: Toxicology
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Mottahedzadeh R, Khatamsaz S, Mokhtari M J. Comparison of the effect of lead acetate and Sodium Sulfide poisoning on Npm1 gene expression in adult male rats. J Gorgan Univ Med Sci 2021; 23 (1) :31-37
URL: http://goums.ac.ir/journal/article-1-3794-en.html
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Volume 23, Issue 1 (3-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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