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:: Volume 22, Issue 3 (10-2020) ::
J Gorgan Univ Med Sci 2020, 22(3): 73-82 Back to browse issues page
Effect of endurance training and glucosamine consumption on gene expression of IGF-1 and IGFBP-3 in knee tissue of mice with acuteosteoarthritis
Mahnaz Alinejad1 , Alireza Barari 2, Asieh Abbasi Daloii3 , Parvin Farzanegi4
1- Ph.D Candidate in Sport Physiology _ Cardiovascular and Respiration, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Associate Professor, Department of Sport Physiology , Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , alireza54.barari@gmail.com
3- Associate Professor, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
4- Associate Professor, Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran
Abstract:   (9922 Views)
Background and Objective: Knee osteoarthritis is one of the most common causes of musculoskeletal disability. This study was performed to determine the effect of endurance training and glucosamine consumption on Insulin-Like Growth Factor 1 (IGF-1) and Insulin-Like Growth Factor Binding Protein 3 (IGFBP-3) gene expression in knee tissue of mice with osteoarthritis.
Methods: This experimental study was performed on 30 adult male Wistar rats. Induction of osteoarthritis of the knee was performed by surgery. The animals were randomly allocated into 5 groups and each group consisted of 6 mice. Groups include: Control, Saline, glucosamine, train and train-glucosamine. The training program for eight weeks, three sessions per week and included 29-25 minutes running on a treadmill at a speed of 15 m/ min for the first week, and each week one meter per minute was added to reach 22 m/min for the eighth week. All animals followed 12-hour fasting and 48 hours after the last session of the session, anesthetized with intraperitoneal injection of Ketamine and Xylosin. The expression of IGF-1 and IGFBP-3 of cartilage was measured by Real Time PCR.
Results: Expression of IGF-1 and IGFBP-3 increased to be 22% and 6% in control group compared to Saline group. Also, level of IGF-1 in exercise groups, glucosamine and exercise-glucosamine group was significantly increased compared to control group (P<0.05). The gene expression of IGFBP-3 in the exercise-glucosamine group was significantly increased compared to the control group (P<0.05).
Conclusion: Probably Chondrocytes secrete higher levels of IGF-1 during tissue damage that causes the structural synthesis of collagen 2 and proteoglycans and Finally, endurance training with consumption of glucose amine increases the expression of IGFBP-3 gene.
Keywords: Endurance Training [MeSH], IGF-1 [MeSH], IGFBP-3 [MeSH], Osteoarthritis [MeSH]
Article ID: Vol22-40
Full-Text [PDF 338 kb]   (11364 Downloads)    
Type of Study: Original Articles | Subject: Orthopaedics
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Alinejad M, Barari A, Abbasi Daloii A, Farzanegi P. Effect of endurance training and glucosamine consumption on gene expression of IGF-1 and IGFBP-3 in knee tissue of mice with acuteosteoarthritis. J Gorgan Univ Med Sci 2020; 22 (3) :73-82
URL: http://goums.ac.ir/journal/article-1-3659-en.html
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Volume 22, Issue 3 (10-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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