[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aims and Scope
Content Coverage
Conflict of Interest
Publication Ethics
Editorial Board::
Executive Members::
Instruction to Authors::
Peer Review::
Articles Archive::
Current Issue
All Issues
Indexing Databases::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 22, Issue 1 (3-2020) ::
J Gorgan Univ Med Sci 2020, 22(1): 82-87 Back to browse issues page
Prevalancy of cagA and babA of Helicobacter pylori isolated from gastric atrophic patients
Sekineh Safarnejad1 , Amin Talebi Bezmin Abadi * 2
1- M.Sc Student in Medical Bacteriology, Department of Bacteriology, Medical Sciences, Tarbiat Modares University, Tehran, Iran.
2- Assistant Professor, Department of Bacteriology, Medical Sciences, Tarbiat Modares University, Tehran, Iran , amin.talebi@modares.ac.ir
Abstract:   (10023 Views)
Background and Objective: The infection of Helicobacter pylori (H. pylori) is one of the most complex items addressed in the clinical microbiology. Although H. pylori positive subjects are bound to develop into atrophic gastritis, current body of evidences is rare. Due to the high prevalence of this bacterium worldwide, finding the true virulence factors as biomarkers for severe gastroduodenal diseases was the priority in recent researches. This study was carried out to determine the prevalancy of cagA and babA of Helicobacter pylori isolated from gastric atrophic patients.
Methods: This descriptive – analytical study was conducted on 100 patients with gastroduodenal disorders in Labafinejad hospital in Tehran, Iran during 2018. Identification of each patient and also bacterial isolation were undertaken according to the standard protocols.
Results: H. pylori were isolated in 23% of patients. 10 patients affected by atrophic gastritis followd by gastric ulcer (7 patients) and acute gastritis (6 patients). In totally, the rate of cagA gene and babA in H. pylori isolated with positive results was 52% and 34%, respectively. There was a significant association between the presence of cagA positive strains and patients with gastric atrophic (P<0.05). The babA gene did not correlate with the presence of gastric atrophic patients.
Conclusion: This study showed that various carrying cagA positive H. pylori can be recovered from patients with gastric atrophy.
Keywords: Helicobacter pylori, cagA, babA, Gastric cancer, Gastric atrophy
Article ID: Vol22-12
Full-Text [PDF 198 kb]   (14920 Downloads)    
Type of Study: Original Articles | Subject: Microbiology
References
1. Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev. 2006 Jul; 19(3): 449-90. doi: 10.1128/CMR.00054-05
2. Marcus EA, Scott DR. Gastric colonization by H. pylori. In: Kim N. Helicobacter pylori. Chap 2. 1st ed. Singapore: Springer. 2016; pp: 23-34. doi: 10.1007/978-981-287-706-2_2
3. Malfertheiner P, Mégraud F, O'Morain C, Hungin AP, Jones R, Axon A, et al. Current concepts in the management of Helicobacter pylori infection--the Maastricht 2-2000 Consensus Report. Aliment Pharmacol Ther. 2002 Feb; 16(2): 167-80. doi: 10.1046/j.1365-2036.2002.01169.x
4. Hatakeyama M, Higashi H. Helicobacter pylori CagA: a new paradigm for bacterial carcinogenesis. Cancer Sci. 2005 Dec; 96(12): 835-43. doi: 10.1111/j.1349-7006.2005.00130.x
5. Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984 Jun; 1(8390): 1311-15. doi: 10.1016/s0140-6736(84)91816-6
6. Graham DY. Illusions regarding Helicobacter pylori clinical trials and treatment guidelines. Gut. 2017 Dec; 66(12): 2043-46. doi: 10.1136/gutjnl-2017-314744
7. Howden CW, Hunt RH. Guidelines for the management of Helicobacter pylori infection. Ad Hoc Committee on Practice Parameters of the American College of Gastroenterology. Am J Gastroenterol. 1998 Dec; 93(12): 2330-38. doi: 10.1111/j.1572-0241.1998.00684.x
8. Zagari RM, Romano M, Ojetti V, Stockbrugger R, Gullini S, Annibale B, et al. Guidelines for the management of Helicobacter pylori infection in Italy: The III Working Group Consensus Report 2015. Dig Liver Dis. 2015 Nov; 47(11): 903-12. doi: 10.1016/j.dld.2015.06.010
9. Cai H, Li W, Shu X, Peng K, Zhang Y, Jiang M. Genetic variation of Helicobacter pylori in the oral cavity and stomach detected using thymine adenine cloning in children with chronic gastritis. Pediatr Infect Dis J. 2014 Jan; 33(1): e1-6. doi: 10.1097/INF.0000000000000017
10. Draper JL, Hansen LM, Bernick DL, Abedrabbo S, Underwood JG, Kong N, et al Fallacy of the Unique Genome: Sequence Diversity within Single Helicobacter pylori Strains. American Society for Microbilogy. 2017; 8(1): e02321-16. doi: 10.1128/mBio.02321-16
11. Mocellin S, Verdi D, Pooley KA, Nitti D. Genetic variation and gastric cancer risk: a field synopsis and meta-analysis. Gut. 2015 Aug; 64(8): 1209-19. doi: 10.1136/gutjnl-2015-309168
12. Ajami A, Shadman M, Rafiei A, Hosseini V, Talebi Bezmin Abadi A, Alizadeh A, et al. Prevalence of EPIYA motifs in Helicobacter pylori strains isolated from patients with gastroduodenal disorders in northern Iran. Reseach in Molecular Medicine. 2013; 1(1): 29-34. doi: 10.18869/acadpub.rmm.1.1.29
13. Honarmand-Jahromy S, Siavoshi F, Malekzadeh R, Sattari TN, Latifi-Navid S. Multiple repeats of Helicobacter pylori CagA EPIYA-C phosphorylation sites predict risk of gastric ulcer in Iran. Microb Pathog. 2015 Dec; 89: 87-92. doi: 10.1016/j.micpath.2015.09.005
14. Lin CJ, Liao WC, Lin HJ, Hsu YM, Lin CL, Chen YA, et al. Statins Attenuate Helicobacter pylori CagA Translocation and Reduce Incidence of Gastric Cancer: In Vitro and Population-Based Case-Control Studies. PLoS One. 2016 Jan; 11(1): e0146432. doi: 10.1371/journal.pone.0146432
15. Ackermark P, Kuipers EJ, Wolf C, Breumelhof R, Seldenrijk CA, Timmer R, et al. Colonization with cagA-positive Helicobacter pylori strains in intestinal metaplasia of the esophagus and the esophagogastric junction. Am J Gastroenterol. 2003 Aug; 98(8): 1719-24. doi: 10.1111/j.1572-0241.2003.07585.x
16. Amieva MR, Vogelmann R, Covacci A, Tompkins LS, Nelson WJ, Falkow S. Disruption of the epithelial apical-junctional complex by Helicobacter pylori CagA. Science. 2003 May; 300(5624): 1430-34. doi: 10.1126/science.1081919
17. Peterson WL. Helicobacter pylori and peptic ulcer disease. N Engl J Med. 1991 Apr; 324(15): 1043-48. doi: 10.1056/NEJM199104113241507
18. Park JY, Forman D, Waskito LA, Yamaoka Y, Crabtree JE. Epidemiology of Helicobacter pylori and CagA-Positive Infections and Global Variations in Gastric Cancer. Toxins (Basel). 2018 Apr; 10(4) pii: E163. doi: 10.3390/toxins10040163
19. Ansari S, Yamaoka Y. Helicobacter pylori BabA in adaptation for gastric colonization. World J Gastroenterol. 2017 Jun; 23(23): 4158-69. doi: 10.3748/wjg.v23.i23.4158
20. Singh R, Taneja VL, Verma KS, Dung R. Chronic Gastritis: Helicobacter pylori Infection: A Clinico-Endoscopic and Histological evaluation. Global Journal for Research Analysis. 2017 Feb; 6(2): 32-35.
21. Chomvarin C, Namwat W, Chaicumpar K, Mairiang P, Sangchan A, Sripa B, et al. Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients. Int J Infect Dis. 2008 Jan; 12(1): 30-36. doi: 10.1016/j.ijid.2007.03.012
22. Mégraud F, Lehours P. Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin Microbiol Rev. 2007 Apr; 20(2): 280-322. doi: 10.1128/CMR.00033-06
23. Huang JY, Goers Sweeney E, Guillemin K, Amieva MR. Multiple Acid Sensors Control Helicobacter pylori Colonization of the Stomach. PLoS Pathog. 2017 Jan; 13(1): e1006118. doi: 10.1371/journal.ppat.1006118
24. Rhee KH, Park JS, Cho MJ. Helicobacter pylori: bacterial strategy for incipient stage and persistent colonization in human gastric niches. Yonsei Med J. 2014 Nov; 55(6): 1453-66. doi: 10.3349/ymj.2014.55.6.1453
25. Sgouras DN, Trang TT, Yamaoka Y. Pathogenesis of Helicobacter pylori Infection. Helicobacter. 2015 Sep;20 Suppl 1:8-16. doi: 10.1111/hel.12251
26. Hong J-B, Zuo W, Wang A-J, Lu N-H. Helicobacter pylori infection synergistic with IL-1β gene polymorphisms potentially contributes to the carcinogenesis of gastric cancer. Int J Med Sci. 2016; 13(4): 298-303. doi: 10.7150/ijms.14239
27. Zhang R-G, Duan G-C, Fan Q-T, Chen S-Y. Role of Helicobacter pylori infection in pathogenesis of gastric carcinoma. World J Gastrointest Pathophysiol. 2016 Feb; 7(1): 97-107. doi: 10.4291/wjgp.v7.i1.97
28. Wang F, Meng W, Wang B, Qiao L. Helicobacter pylori-induced gastric inflammation and gastric cancer. Cancer Lett. 2014 Apr; 345(2): 196-202. doi: 10.1016/j.canlet.2013.08.016
29. Bugaytsova JA, Björnham O, Chernov YA, Gideonsson P, Henriksson S, Mendez M, et al. Helicobacter pylori Adapts to Chronic Infection and Gastric Disease via pH-Responsive BabA-Mediated Adherence. Cell Host Microbe. 2017 Mar; 21(3): 376-89. doi: 10.1016/j.chom.2017.02.013
30. Gerhard M, Lehn N, Neumayer N, Borén T, Rad R, Schepp W, et al. Clinical relevance of the Helicobacter pylori gene for blood-group antigen-binding adhesin. Proc Natl Acad Sci U S A. 1999 Oct; 96(22): 12778-83. doi: 10.1073/pnas.96.22.12778
31. Gold BD, van Doorn LJ, Guarner J, Owens M, Smith DP, Song Q, et al. Genotypic, Clinical, and Demographic Characteristics of Children Infected with Helicobacter pylori. J Clin Microbiol. 2001 Apr; 39(4): 1348-52. doi: 10.1128/JCM.39.4.1348-1352.2001
32. Rafeey M, Ghotaslou R, Milani M, Farokhi N, Ghojazadeh M. Association between Helicobacter pylori, cagA, and vacA Status and Clinical Presentation in Iranian Children. Iran J Pediatr. 2013 Oct; 23(5): 551-56.
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Safarnejad S, Talebi Bezmin Abadi A. Prevalancy of cagA and babA of Helicobacter pylori isolated from gastric atrophic patients. J Gorgan Univ Med Sci 2020; 22 (1) :82-87
URL: http://goums.ac.ir/journal/article-1-3570-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 22, Issue 1 (3-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4660
Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)