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:: Volume 21, Issue 3 (10-2019) ::
J Gorgan Univ Med Sci 2019, 21(3): 41-48 Back to browse issues page
Effect of glucose on improvement of BM-MSC differentiation into cardiomyocyte - like cells
Sara Raisolsadati1 , Abdoljalal Marjani2 , Safoura Khajeniazi * 3
1- M.Sc Student in Medical Biotechnology, Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
2- Professor, Metabolic Disorders Research Center, Department of Biochemistry and Biophysics, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3- Assistant Professor, Stem Cell Research Center, Department of Medical Technology, Faculty of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran , niazie80@gmail.com
Abstract:   (6505 Views)
Background and Objective: Cardiovascular diseases and heart failure are major diseases in developed countries. Stem cells showed specific features to play an important role in heart disease treatment. One of the most common compounds has been used to induce differentiation of stem cells into cardiomyocyte is 5-Azacytidine. Medium contents of cell culture such as different glucose concentrations also influence on morphology and function of final differentiated cells. This study was done to evaluate the effect of glucose on improvement of BM-MSC differentiation into cardiomyocyte - like cells.
Methods: In this experimental study, effect of two different glucose concentrations (5 and 25mM) on the mesenchymal stem cells differentiation (MSCs) to cardiomyocytes during 21 days was evaluated. Bone marrow MSCs (BM MSCs) seeded in differentiation medium which treated with 5-aza and 5 & 25mM glucose concentrations. In next step, total RNA was extracted and cDNA synthesis was carried out. Finally, quantitative polymerase chain reaction (Q-PCR) was done to determine level of cardiac-specific markers during differentiation process including Connexin43, α-cardiac actin, TroponinT and TroponinI.
Results: Level of cardiac-specific markers during differentiation of mesenchymal stem cells to cardiomyocyte including Connexin43, α-cardiac actin, TroponinT and TroponinI in 5 and 25 mM of glucose concentration was diferent, but this diference was not significant.
Conclusion: Our results showed that two concentrations of glucose (5, 25mM) have no remarkable effect on the expression of cardiac markers during differentiation of bone marrow mesenchymal stem cells to cardiomyocyte.
Keywords: Cardiomyocyte, Mesenchymal stem cell, Glucose, 5-Azacytidine, Cardiac-specific markers
Full-Text [PDF 321 kb]   (15385 Downloads)    
Type of Study: Original Articles | Subject: Stem Cells
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Raisolsadati S, Marjani A, Khajeniazi S. Effect of glucose on improvement of BM-MSC differentiation into cardiomyocyte - like cells. J Gorgan Univ Med Sci 2019; 21 (3) :41-48
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Volume 21, Issue 3 (10-2019) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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