Liver toxicity of multiwall carbon nanotubes in rats

Kooravand Bardpareh , Mitra; Noori , Ali

[Home ] [Archive]

[ فارسی ]

Journal of Gorgan University of Medical Sciences

Main Menu

Home

Journal Information

Editorial Board

Executive Members

Instruction to Authors

Peer Review

Articles Archive

Indexing Databases

Contact Us

Site Facilities

Search in website

Receive site information

Volume 20, Issue 4 (12-2018)

J Gorgan Univ Med Sci 2018, 20(4): 36-43

Back to browse issues page

Liver toxicity of multiwall carbon nanotubes in rats

Mitra Kooravand Bardpareh¹

, Ali Noori ^*

1- M.Sc in Animal Physiology, Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2- Assistant Professor, Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran , ali.noori55@gmail.com

Abstract: (6615 Views)

Background and Objective: Multiwall carbon nanotubes nowadays have multiple uses in the field of drug and gene delivery and other biological fields, and it is necessary to study their potential toxicity on organisms due to unique properties of these nanostructures. This study was conducted to determine the toxicity of multi-wall carbon nanotubes functionalized with carboxylic groups on the function and structure of the rats liver tissue.
Methods: In this experimental study, 50 mature female Wistar Rats were randomly allocated into five groups including the control group of normal saline and Tween and treatment groups 2.5, 5, 10, 20 mg/kg/bw concentrations of multi-wall carbon nanotubes functionalized with carboxylic group with diameter less than 8 nm and length 30 micrometers that was received in 8 steps, intraperitoneally. Blood sampling was performed in two steps (The first stage was one day after the last injection and the second stage was 20 days after the last injection). The level of activity of the aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) enzymes and the amount of malondialdehyde were measured in serum. By preparing the tissue sections of the liver, a number of rats in each group (after 20 days from the last injection) with hematoxylin-eosin staining, the tissue structure of the liver was examined by optical microscopy. Animals were weighed before and after treatment.
Results: In the first stage, only the mean of AST activity at 5 mg/kg/bw concentration was significantly increased (P<0.05). In the second stage, ALP activity was significantly reduced (P<0.05) in all doses higher than 2.5 mg/kg/bw and the activity of AST and ALT in doses of 5 and 10 mg/kg/bw was significantly reduced (P<0.05) and in the dose of 2.5 mg/kg/bw was significantly increased (P<0.05). Histologic studies revealed disturbances such as degeneration of the vein wall of the lobular center, degeneration of the nucleus and hepatocyte lysis with severe atrophy, irregularity and dilatation of the sinusoids and accumulation of inflammatory cells in the treatment groups in dose-dependent manner. Based on the above findings the most disturbances were related to the 20 mg/kg/bw concentration.
Conclusion: It seems that multi-wall carbon nanotubes functionalized with carboxylic group, even in small amounts (2.5 and 5 mg/kg/bw) after 20 days, are toxic on the liver and cause liver tissue and function impairment.

Keywords: Multiwall Carbon Nanotubes, Liver Enzymes, Liver Tissue Structure, Toxicity, Rat

Full-Text [PDF 419 kb] (19470 Downloads)

Type of Study: Original Articles | Subject: Medical Biotecnology

References

1. Hillegass JM, Shukla A, Lathrop SA, MacPherson MB, Fukagawa NK, Mossman BT. Assessing nanotoxicity in cells in vitro. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010; 2(3): 219-31. doi: 10.1002/wnan.54

2. De Volder MF, Tawfick SH, Baughman RH, Hart AJ. Carbon nanotubes: present and future commercial applications. Science. 2013 Feb; 339(6119): 535-39. doi: 10.1126/science.1222453

3. Yomogida Y, Tanaka T, Zhang M, Yudasaka M, Wei X, Kataura H. Industrial-scale separation of high-purity single-chirality single-wall carbon nanotubes for biological imaging. Nat Commun. 2016 Jun; 7: 12056. doi: 10.1038/ncomms12056

4. Vlastou E, Gazouli M, Ploussi A, Platoni K, Efstathopoulos EP. Nanoparticles: nanotoxicity aspects. J Phys Conf Ser. 2017; 931(17): 012020. doi: 10.1088/1742-6596/931/1/012020

5. Lacerda L, Bianco A, Prato M, Kostarelos K. Carbon nanotubes as nanomedicines: from toxicology to pharmacology. Adv Drug Deliv Rev. 2006 Dec; 58(14): 1460-70. doi: 10.1016/j.addr.2006.09.015

6. Abdal Dayem A, Hossain MK, Lee SB, Kim K, Saha SK, Yang GM, et al. The Role of Reactive Oxygen Species (ROS) in the Biological Activities of Metallic Nanoparticles. Int J Mol Sci. 2017 Jan; 18(1) pii: E120. doi: 10.3390/ijms18010120

7. Prakash S, Malhotra M, Shao W, Tomaro-Duchesneau C, Abbasi S. Polymeric nanohybrids and functionalized carbon nanotubes as drug delivery carriers for cancer therapy. Adv Drug Deliv Rev. 2011 Nov; 63(14-15): 1340-51. doi: 10.1016/j.addr.2011.06.013

8. Yang ST, Wang X, Jia G, Gu Y, Wang T, Nie H, et al. Long-term accumulation and low toxicity of single-walled carbon nanotubes in intravenously exposed mice. Toxicol Lett. 2008 Oct; 181(3): 182-9. doi: 10.1016/j.toxlet.2008.07.020

9. Clichici S, Biris AR, Tabaran F, Filip A. Transient oxidative stress and inflammation after intraperitoneal administration of multiwalled carbon nanotubes functionalized with single strand DNA in rats. Toxicol Appl Pharmacol. 2012 Mar; 259(3): 281-92. doi: 10.1016/j.taap.2012.01.004

10. Schipper ML, Nakayama-Ratchford N, Davis CR, Shi Kam NW, Chu P, Liu Z, et al. A pilot toxicology study of single-walled carbon nanotubes in a small sample of mice. Nature Nanotechnology. 2008; 3(8): 216-21.

11. Warheit DB, Laurence BR, Reed KL, Roach DH, Reynolds GA, Webb TR. Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats. Toxicol Sci. 2004 Jan; 77(1): 117-25. doi: 10.1093/toxsci/kfg228

12. Jain S, Thakare VS, Das M, Godugu C, Jain AK, Mathur R, et al. Toxicity of multiwalled carbon nanotubes with end defects critically depends on their functionalization density. Chem Res Toxicol. 2011 Nov; 24(11): 2028-39. doi: 10.1021/tx2003728

13. Sharma M, Nikota J, Halappanavar S, Castranova V, Rothen-Rutishauser B, Clippinger AJ. Predicting pulmonary fibrosis in humans after exposure to multi-walled carbon nanotubes (MWCNTs). Arch Toxicol. 2016 Jul; 90(7): 1605-22. doi: 10.1007/s00204-016-1742-7

14. Ong LC, Chung FF, Tan YF, Leong CO. Toxicity of single-walled carbon nanotubes. Arch Toxicol. 2016 Jan; 90(1): 103-18. doi: 10.1007/s00204-014-1376-6

15. Poland CA, Duffin R, Kinloch I, Maynard A, Wallace WA, Seaton A, et al. Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study. Nat Nanotechnol. 2008 Jul; 3(7): 423-28. doi: 10.1038/nnano.2008.111

16. Singh R, Pantarotto D, Lacerda L, Pastorin G, Klumpp C, Prato M, et al. Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers. PNAS 2006 Feb; 103(9): 3357-62. https://doi.org/10.1073/pnas.0509009103

17. Shvedova AA, Kisin ER, Murray AR, Kommineni C, Castranova V, Fadeel B. Increased accumulation of neutrophils and decreased fibrosis in the lung of NADPH oxidase-deficient C57BL/6 mice exposed to carbon nanotubes. Toxicol Appl Pharmacol. 2008 Sep; 231(2): 235-40. doi: 10.1016/j.taap.2008.04.018

18. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983 Jun; 16(2): 109-10.

19. Heidari-Rarani M, Noori A, Ghodousi A. Effects of methamphetamine on pituitary gonadal axis and spermatogenesis in mature male rats. Zahedan J Res Med Sci. 2014; 16(12): 35-40.

20. Zhao X, Liu R. Recent progress and perspectives on the toxicity of carbon nanotubes at organism, organ, cell, and biomacromolecule levels. Environ Int. 2012 Apr; 40: 244-55. doi: 10.1016/j.envint.2011.12.003

21. Liu Z, Cai W, He L, Nakayama N, Chen K, Sun X, et al. In vivo biodistribution and highly efficient tumour targeting of carbon nanotubes in mice. Nature Nanotechnology. 2007; 2: 47-52.

22. Muller J, Decordier I, Hoet PH, Lombaert N, Thomassen L, Huaux F, et al. Clastogenic and aneugenic effects of multi-wall carbon nanotubes in epithelial cells. Carcinogenesis. 2008 Feb; 29(2): 427-33. doi: 10.1093/carcin/bgm243

23. Lacerda L, Ali-Boucetta H, Herrero MA, Pastorin G, Bianco A, Prato M, et al. Tissue histology and physiology following intravenous administration of different types of functionalized multiwalled carbon nanotubes. Nanomedicine (Lond). 2008 Apr; 3(2): 149-61. doi: 10.2217/17435889.3.2.149

24. Wang J, Sun P, Bao Y, Liu J, An L. Cytotoxicity of single-walled carbon nanotubes on PC12 cells. Toxicol In Vitro. 2011 Feb; 25(1): 242-50. doi: 10.1016/j.tiv.2010.11.010

25. Ge C, Du J, Zhao L, Wang L, Liu Y, Li D, et al. Binding of blood proteins to carbon nanotubes reduces cytotoxicity. PNAS 2011; 108(41): 16968-73. https://doi.org/10.1073/pnas. 1105270108

26. Smart SK, Cassady AI, Lu GQ, Martin DJ. The biocompatibility of carbon nanotubes. Carbon. 2006; 44(6): 1034-47. https://doi.org/10.1016/j.carbon.2005.10.011

Send email to the article author

Mendeley

Zotero

RefWorks

Kooravand Bardpareh M, Noori A. Liver toxicity of multiwall carbon nanotubes in rats. J Gorgan Univ Med Sci 2018; 20 (4) :36-43
URL: http://goums.ac.ir/journal/article-1-3538-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 20, Issue 4 (12-2018)

Back to browse issues page

Persian site map - English site map - Created in 0.04 seconds with 36 queries by YEKTAWEB 4660