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:: Volume 20, Issue 4 (12-2018) ::
J Gorgan Univ Med Sci 2018, 20(4): 31-35 Back to browse issues page
Effect of gestational diabetes on expersion of desmocollin 2 and collagen structural genes in C57BL mouse embryo heart
Sara Pasban Bovanlo1 , Masoud Golalipour2 , Kamran Haidari3 , Mohammad Jafar Golalipour * 4
1- M.Sc Student of Anatomy, Faculty of Medicine, Golestan University of Medical Scineces, Gorgan, Iran. pasbansara@gmail.com
2- Assistant Professor, Medical Cellular and Molecular Research Center, Golestan University of Medical Scineces, Gorgan, Iran
3- Associate Professor, Department of Anatomical Sciences, Faculty of Medicine, Golestan University of Medical Scineces, Gorgan, Iran
4- Professor, Gorgan Congenital Malformations Research Center, Golestan University of Medical Scineces, Gorgan, Iran , mjgolalipour@yahoo.com
Abstract:   (10117 Views)
Background and Objective: Gestational diabetes (GDM) is a metabolic disorder which is caused by insufficient secretion of insulin. GDM is a risk factor for embryo during pregnancy and it is possible leads to congenital heart defects (CHD). Some of these defects may be due to a change in the expression of some of the important structural genes in the heart. Desmocollin 2 and collagen structural genes have important role in the cell adhesion of the cardiomyocytes.This study was done to determine the effect of gestational diabetes on expersion of desmocollin 2 and col5a2 structural genes in C57BL mouse embryo heart.
Methods: In this experimental study, 12 adult female and six adult male C57BL mice were used.After mating of the animals and observation of the vaginal plug, the female mice with vaginal plug were randomly divided into diabetic and control groups. At the first day of pregnancy, Induction of gestational diabetes mellitus in dams in the diabetic group was performed by the intraperitoneal (IP) injection of Streptozotocin with a dose of 150 mg / kg body weight per day in GD1. While in the control group, only citrate buffer was injected.Cesarean Surgery was done at E11.5 and embryo's heart was extracted from the body.Extraction of RNA, cDNA, and quantitative measurements of the amount of RNA were performed using Real -Time PCR.
Results: Induction of gestational diabetes increased the expersion of desmocollin 2 and col5a2 structural genes in compared to controls, althought only the expersion of desmocollin 2 gene was significant (P<0.05).
Conclusion: We suggest that the induction of DM lead to upregulation of structural genes primarily including desmocollin 2 and col5a2 in embryos heart development.
Keywords: Gestational diabetes mellitus, Col5a2 gene, Dsc2 gene, Heart development, C57BL mouse
Full-Text [PDF 231 kb]   (19238 Downloads)    
Type of Study: Original Articles | Subject: Embryology
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Pasban Bovanlo S, Golalipour M, Haidari K, Golalipour M J. Effect of gestational diabetes on expersion of desmocollin 2 and collagen structural genes in C57BL mouse embryo heart. J Gorgan Univ Med Sci 2018; 20 (4) :31-35
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Volume 20, Issue 4 (12-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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