[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 4 (Winter 2020) ::
J Gorgan Univ Med Sci 2020, 21(4): 107-113 Back to browse issues page
Comparison of the in vitro effect of Cannabis sativa Essential Oil with Albendazole on Protoscolices of Hydatid Cyst
Amir Reza Youssefi1 , Mohammad Reza Youssefi * 2, Mohaddeseh Abouhosseini Tabari3
1- Academic Instrcrutor, Department of Veterinary Parasitology, Babol Branch, Islamic Azad University, Babol, Iran
2- Associate Professor, Department of Veterinary Parasitology, Babol Branch, Islamic Azad University, Babol, Iran , youssefi929@hotmail.com
3- Assistant Professor, Department of Pharmacology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
Abstract:   (948 Views)
Background and Objective: Echinococcosis garnulosus is a major zoonotic disease, and surgery is the best treatment for it. So far, several chemical scolicidals have been used to prevent protoscolices leakage during surgery, but due to their adverse effects were not well-received. Therefore, using medicinal plants or compounds driven from them as alternatives were taken into consideration. This study was conducted to compare the in vitro effect of Cannabis sativa essential oil with albendazole on protoscolices of hydatid cyst.
Methods: In this descriptive-analytic study, essential oil of C. sativa was prepared from aerial parts of the plant in flowering season in university of Camerino, Italy, and Gas chromatography – mass spectrometry (GC-MS) analysis was performed. Protoscolices were extracted from the livers infected with hydatid cyst and were exposed to different concentrations of C. sativa (1, 2, 5 and 10 μg/ml) for 10, 30, 60 and 120 minutes. The viability of protoscolices was measured by 0.1% eosin staining. Albendazole was used as a standard medicinal drug.
Results: Essential oil of C. sativa at all tested concentrations led to significant mortality in protoscolices which showed a direct relation with increase in concentration (P<0.05). The highest concentration of albendazole after 2 h led to 13.24% mortality rate while, this amount with C. sativa essential oil at 2µg/ml reached to 20.9%, and at 10µg/ml reached to 26.08%.
Conclusion: This study showed that C. sativa essential oil has promising scolicidal effect compared to albendazole and can be suggested as a natural protoscolicidal agent.
Keywords: Echinococcus granulosus, Green drug, Essential oil, Albendazole
Full-Text [PDF 234 kb]   (136 Downloads)    
Type of Study: Original Articles | Subject: Parasitology
1. Abunna F, Fentaye S, Megersa B, Regassa A. Prevalence of bovine hydatidosis in Kombolcha ELFORA abattoir, North Eastern Ethiopia. Biomedical and Life Sciences. 2012; 2(4): 281-86. doi: 10.4236/ojas.2012.24038
2. Dakkak A. Echinococcosis/hydatidosis: a severe threat in Mediterranean countries. Vet Parasitol. 2010 Nov; 174(1-2): 2-11. doi: 10.1016/j.vetpar.2010.08.009
3. Andalib Aliabadi Z, Berenji F, Fata A, Jarahi L. Human Hydatidosis/Echinococosis in North Eastern Iran from 2003-2012. Iran J Parasitol. 2015 Oct-Dec; 10(4): 658-62.
4. Topcu O, Sumer Z, Tuncer E, Aydin C, Koyuncu A. Efficacy of chlorhexidine gluconate during surgery for hydatid cyst. World J Surg. 2009 Jun; 33(6): 1274-80. doi: 10.1007/s00268-009-9971-z
5. Hazrati Tappe K, Mousavi SJ, Barazesh A. Prevalence and fertility of hydatid cyst in slaughtered livestock of Urmia city, Northwest Iran. J Parasitol Vector Biol. 2011; 3(2): 29-32.
6. Todorov T, Vutova K, Mechkov G, Georgiev P, Petkov D, Tonchev Z, Nedelkov G. Chemotherapy of human cystic echinococcosis: comparative efficacy of mebendazole and albendazole. Ann Trop Med Parasitol. 1992 Feb; 86(1): 59-66. doi: 10.1080/00034983.1992.11812631
7. Small E., Cronquist A. A practical and natural taxonomy for cannabis. Taxon. 1976; 25(4): 405-35. doi: 10.2307/1220524
8. Borhade SS., Chemical Composition and Characterzation of Hemp(Cannabis sativa) seed oil and essential fatty acids by HPLC Method. Arch Appl Sci Res. 2013; 5(1): 5-8.
9. Moon T, Wilkinson JM, Cavanagh HM. Antiparasitic activity of two Lavandula essential oils against Giardia duodenalis, Trichomonas vaginalis and Hexamita inflata. Parasitol Res. 2006 Nov; 99(6): 722-28. doi: 10.1007/s00436-006-0234-8
10. Anthony JP, Fyfe L, Smith H. Plant active components - a resource for antiparasitic agents? Trends Parasitol. 2005 Oct; 21(10): 462-68. doi: 10.1016/j.pt.2005.08.004
11. Guarrera PM. Traditional antihelmintic, antiparasitic and repellent uses of plants in Central Italy. J Ethnopharmacol. 1999 Dec; 68(1-3): 183-92.
12. Esperandim VR, da Silva Ferreira D, Sousa Rezende KC, Magalhães LG, Medeiros Souza J, Pauletti PM, et al. In vitro antiparasitic activity and chemical composition of the essential oil obtained from the fruits of Piper cubeba. Planta Med. 2013 Nov; 79(17): 1653-55. doi: 10.1055/s-0033-1351022
13. Benelli G, Pavela R, Petrelli R, Cappellacci L, Santini G, Fiorini D, et al. The essential oil from industrial hemp (Cannabis sativa L.) by-products as an effective tool for insect pest management in organic crops. Industrial Crops and Products. 2018; 122: 308-15. https://doi.org/10.1016/j.indcrop.2018.05.032
14. Moazeni M, Saharkhiz MJ, Hosseini AA. In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces. Vet Parasitol. 2012 Jun; 187(1-2): 203-8. doi: 10.1016/j.vetpar.2011.12.025
15. Elissondo MC, Albani CM, Gende L, Eguaras M, Denegri G. Efficacy of thymol against Echinococcus granulosus protoscoleces. Parasitol Int. 2008 Jun; 57(2): 185-90. doi: 10.1016/j.parint.2007.12.005
16. María AC, Celina EM. Efficacy of albendazole in combination with thymol against Echinococcus multilocularis protoscoleces and metacestodes. Acta Trop. 2014 Dec; 140: 61-7. doi: 10.1016/j.actatropica.2014.08.007
17. Mahmoudvand H, Tavakoli Oliaei R, Mirbadie SR, Kheirandish F, Tavakoli Kareshk A, Ezatpour B, et al. Efficacy and Safety of Bunium Persicum (Boiss) to Inactivate Protoscoleces during Hydatid Cyst Operations. Surg Infect (Larchmt). 2016 Dec; 17(6): 713-19. doi: 10.1089/sur.2016.010
18. Mahmoudvand H, Mirbadie SR, Ghasemi Kia M, Badparva E, Shamsadini Lori S, Fasihi Harandi M. Efficacy of Pistacia khinjuk Fruits on Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model. Iran J Parasitol. 2016 Jul-Sep; 11(3): 383-88.
19. Hosseini M, Yousefi M, Abouhosseini M. [Comparison of the Effect of Artemisia Sieberi Essential Oil and Albendazole Drug on Protoscolices of Hydatid Cyst under in Vitro Conditions]. J Babol Univ Med Sci. 2017; 19(12): 63-68. doi: 10.18869/acadpub.jbums.19.12.63 [Article in Persian]
20. Rostami A, Taheri M, Gholizadeh M, Seyyedtabaei SJ, Raeghi S, Fallahi S. Scolicidal effect of some herbs on Echinococcus granulosus protoscoleces: a systematic literature review. Herb Med J. 2016; 1(1): 53-59.
21. Sadjjadi SM, Zoharizadeh MR, Panjeshahin MR. In vitro screening of different Allium sativum extracts on hydatid cysts protoscoleces. J Invest Surg. 2008 Nov-Dec; 21(6): 318-22. doi: 10.1080/08941930802348261
22. Maggiore MA, Albanese AA, Gende LB, Eguaras MJ, Denegri GM, Elissondo MC. Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes. Parasitol Res. 2012 Mar; 110(3): 1103-12. doi: 10.1007/s00436-011-2595-x
23. Tabari MA, Youssefi MR, Nasiri M, Hamidi M, Kiani K, Alian Samakkhah S, et al. Towards green drugs against cestodes: Effectiveness of Pelargonium roseum and Ferula gummosa essential oils and their main component on Echinococcus granulosus protoscoleces. Vet Parasitol. 2019 Feb; 266: 84-87. doi: 10.1016/j.vetpar.2018.12.019
24. Monzote L, Stamberg W, Staniek K, Gille L. Toxic effects of carvacrol, caryophyllene oxide, and ascaridole from essential oil of Chenopodium ambrosioides on mitochondria. Toxicol Appl Pharmacol. 2009 Nov; 240(3): 337-47. doi: 10.1016/j.taap.2009.08.001
25. Soares DC, Portella NA, Ramos MF, Siani AC, Saraiva EM. Trans- β -Caryophyllene: An Effective Antileishmanial Compound Found in Commercial Copaiba Oil (Copaifera spp.). Evid Based Complement Alternat Med. 2013; 2013: 761323. doi: 10.1155/2013/761323
26. Polanco-Hernández G, Escalante-Erosa F, García-Sosa K, Rosado ME, Guzmán-Marín E, Acosta-Viana KY, et al. Synergistic Effect of Lupenone and Caryophyllene Oxide against Trypanosoma cruzi. Evid Based Complement Alternat Med. 2013; 2013: 435398. doi: 10.1155/2013/435398
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Youssefi A R, Youssefi M R, Abouhosseini Tabari M. Comparison of the in vitro effect of Cannabis sativa Essential Oil with Albendazole on Protoscolices of Hydatid Cyst. J Gorgan Univ Med Sci. 2020; 21 (4) :107-113
URL: http://goums.ac.ir/journal/article-1-3527-en.html

Volume 21, Issue 4 (Winter 2020) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 4111