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:: Volume 22, Issue 1 (3-2020) ::
J Gorgan Univ Med Sci 2020, 22(1): 50-56 Back to browse issues page
Effect of Oxaliplatin on sperm parameters of 60 days old offspring during pre-pregnancy, pregnancy and lactation period in mice
Moslem Dahmardeh1 , Javad Sadeghinezhad * 2, Zahra Tootian3 , Mojdeh Salehnia4
1- Ph.D Candidate in Comparative Anatomy and Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2- Associate Professor, Department of basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , sadeghinezhad@ut.ac.ir
3- Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
4- Professor, Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
Abstract:   (11669 Views)
Background and Objective: Oxaliplatin is the main agent used in the treatment of colorectal cancers. Oxaliplatin inhibits DNA replication and transcription and to induce apoptosis or necrosis in cancer cells and rapidly dividing cell lines. This study was designed to determine the effect of Oxaliplatin on sperm parameters of 60 days old offspring during pre-pregnancy, pregnancy and lactation period in mice.
Methods: In this experimental study, 32 female NMRI mature mice were randomly allocated into 4 groups. Animals in control group were received 0.2 ml saline intraperitoneally (IP) during 21 days of pre-pregnany, pregnancy and lactation periods. Animals in experimental groups including pre-pregnant, pregnant and lactation groups were received 3 mg/kg oxaliplatin trice a week IP during 21 days before mating, during pregnancy and lactation periods, respectively. At the 60th postnatal day, all the male offspring were euthanized and sperm samples were obtained. Analysis of sperm parameters including count, motility, vitality, maturation and DNA integrity was done.
Results: Sperm count, motility and DNA integrity were significantly reduced in all three groups of Pre-pregnancy, pregnancy and lactation in comparison with control group (P<0.05). Moreover, the percentage of immature and dead sperms were significantly increased in oxaliplatin groups (P<0.05).
Conclusion: Admistration of oxaliplatin induces adverse effect on sperm quality in perinatal period. The greatest effect of this drug is on lactation period. Also, by increasing the time interval for oxaliplatin administration in mice to puberty of offspring, the adverse effects of this drug on the quality of sperm parameters are reduced.
Keywords: Oxaliplatin, Sperm quality, Perinatal period, Spermogram, Mice
Article ID: Vol22-07
Full-Text [PDF 222 kb]   (13791 Downloads)    
Type of Study: Original Articles | Subject: Oncology
References
1. Bleiberg H. Oxaliplatin (L.OHP): a new reality in colorectal cancer. Br J Cancer. 1998 Jun; 77(4): 1-3.
2. Raymond E, Faivre S, Woynarowski JM, Chaney SG. Oxaliplatin: mechanism of action and antineoplastic activity. Semin Oncol. 1998 Apr; 25(2 Suppl 5): 4-12.
3. Levi M, Shalgi R, Brenner B, Perl G, Purim O, Amit L, et al. The impact of oxaliplatin on the gonads: from bedside to the bench. Mol Hum Reprod. 2015 Dec; 21(12): 885-93. doi: 10.1093/molehr/gav055
4. Yu BB, Tong XH, Dong SY, Gu YC, Jiao H, Ji J, et al. [Total flavonoids of litsea coreana decreases the cytotoxicity of oxaliplatin in TM3 Leydig cells via enhancing the function of gap junction]. Zhonghua Nan Ke Xue. 2014 May; 20(5): 400-4. [Article in Chinese]
5. Tong X, Han X, Yu B, Yu M, Jiang G, Ji J, et al. Role of gap junction intercellular communication in testicular leydig cell apoptosis induced by oxaliplatin via the mitochondrial pathway. Oncol Rep. 2015 Jan; 33(1): 207-14. doi: 10.3892/or.2014.3571
6. Jeppesen JB, Østerlind K. Successful twin pregnancy outcome after in utero exposure to FOLFOX for metastatic colon cancer: a case report and review of the literature. Clin Colorectal Cancer. 2011 Dec; 10(4): 348-52. doi: 10.1016/j.clcc.2011.06.003
7. Makoshi Z, Perrott C, Al-Khatani K, Al-Mohaisen F. Chemotherapeutic treatment of colorectal cancer in pregnancy: case report. J Med Case Rep. 2015 Jun; 9: 140. doi: 10.1186/s13256-015-0621-9
8. McQuade RM, Carbone SE, Stojanovska V, Rahman A, Gwynne RM, Robinson AM, et al. Role of oxidative stress in oxaliplatin-induced enteric neuropathy and colonic dysmotility in mice. Br J Pharmacol. 2016 Dec; 173(24): 3502-21. doi: 10.1111/bph.13646
9. Dobrzyńska MM, Gajowik A, Jankowska-Steifer EA, Radzikowska J, Tyrkiel EJ. Reproductive and developmental F1 toxicity following exposure of pubescent F0 male mice to bisphenol A alone and in a combination with X-rays irradiation. Toxicology. 2018 Dec; 410: 142-51. doi: 10.1016/j.tox.2018.10.007
10. World Health Organization. WHO laboratory manual for the examination of human semen and sprm-cervical mucus interaction. 5th ed. Cambridg: Cambridge University Press. 2010; pp: 8-23.
11. Ahmadi A, Sader khanlou RJ, Salami S, Ahmadi A. [Evaluation of sperm quality, maturation and DNA integrity in adult mice treated with sulpiride]. Tehran Univ Med J. 2012; 70(4): 205-11. [Article in Persian]
12. Hammadeh ME, Al Hasani S, Rosenbaum P, Schmidt W, Fischer Hammadeh C. Reactive oxygen species, total antioxidant concentration of seminal plasma and their effect on sperm parameters and outcome of IVF/ICSI patients. Arch Gynecol Obstet. 2008 Jun; 277(6):515-26. doi: 10.1007/s00404-007-0507-1
13. Sadeghi MR, Hodjat M, Lakpour N, Arefi S, Amirjannati N, Modarresi T, et al. Effects of sperm chromatin integrity on fertilization rate and embryo quality following intracytoplasmic sperm injection. Avicenna J Med Biotechnol. 2009; 1(3): 173-80.
14. Seethalakshmi L, Flores C, Kinkead T, Carboni AA, Malhotra RK, Menon M. Effects of subchronic treatment with cis-platinum on testicular function, fertility, pregnancy outcome, and progeny. J Androl. 1992 Jan-Feb; 13(1): 65-74.
15. Bechter R, Haebler R, Ettlin RA, Dixon RL. Testicular toxicity of antineoplastic drugs during postnatal development of the rat. Arch Toxicol Suppl. 1985; 8: 390-93.
16. Favareto AP, de Toledo FC, Kempinas Wde G. Paternal treatment with cisplatin impairs reproduction of adult male offspring in rats. Reprod Toxicol. 2011 Dec; 32(4): 425-33. doi: 10.1016/j.reprotox.2011.10.003
17. Liu M, Hales BF, Robaire B. Effects of four chemotherapeutic agents, bleomycin, etoposide, cisplatin, and cyclophosphamide, on DNA damage and telomeres in a mouse spermatogonial cell line. Biol Reprod. 2014 Apr; 90(4): 72. doi: 10.1095/biolreprod.114.117754
18. Glen CD, Dubrova YE. Exposure to anticancer drugs can result in transgenerational genomic instability in mice. Proceedings of the National Academy of Sciences. 2012 Feb; 109(8): 2984-88. doi: 10.1073/pnas.1119396109
19. Asleiranifam N, Hasanzadeh S, Sam M R, Najafi Tazehkand G R. [The Effects of Hydro-alcoholic Extract of Achillea millefolium on Sperm Parameters and Apoptotic Changes in Cyclophosphamide Treated Mice]. J Mazandaran Univ Med Sci. 2016; 25(133): 77-90. [Article in Persian]
20. Amin A, Abraham C, Hamza AA, Abdalla ZA, Al-Shamsi SB, Harethi SS, et al. A standardized extract of Ginkgo biloba neutralizes cisplatin-mediated reproductive toxicity in rats. J Biomed Biotechnol. 2012 Feb; 2012: 362049. doi: 10.1155/2012/362049
21. Qu N, Kuramasu M, Hirayanagi Y, Nagahori K, Hayashi S, Ogawa Y, et al. Gosha-Jinki-Gan Recovers Spermatogenesis in Mice with Busulfan-Induced Aspermatogenesis. Int J Mol Sci. 2018 Sep; 19(9) pii: E2606. doi: 10.3390/ijms19092606
22. Sikka SC. Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J Androl. 2004 Jan-Feb; 25(1): 5-18. doi: 10.1002/j.1939-4640.2004.tb02751.x
23. Zhu B, Zheng YF, Zhang YY, Cao YS, Zhang L, Li XG, et al. Protective effect of L-carnitine in cyclophosphamide-induced germ cell apoptosis. J Zhejiang Univ Sci B. 2015 Sep; 16(9): 780-87. doi: 10.1631/jzus.B1500015
24. Soleimanirad J, Roshangar L, Abouzaripour M, Daneshi E. [Study of Sperm Parameters and Sperm Fertility in Mice were Exposed to Tamoxifen during Embryonic Development]. J Shahid Sadoughi Univ Med Sci. 2017; 25(2): 101-10. [Article in Persian]
25. Chamorro-Cevallos G, Garduño-Siciliano L, Martínez-Galero E, Mojica-Villegas A, Pages N, Gutiérrez-Salmeán G. The protective effect of dietary Arthrospira (Spirulina) maxima against mutagenicity induced by benzo[alpha]pyrene in mice. J Med Food. 2014 May; 17(5): 527-34. doi: 10.1089/jmf.2013.0109
26. Pasqualotto FF, Sharma RK, Nelson DR, Thomas AJ, Agarwal A. Relationship between oxidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil Steril. 2000 Mar; 73(3): 459-64. doi: 10.1016/s0015-0282(99)00567-1
27. Tremellen K. Oxidative stress and male infertility--a clinical perspective. Hum Reprod Update. 2008 May-Jun; 14(3): 243-58. doi: 10.1093/humupd/dmn004
28. Schaalan MF, Ramadan BK, H Abd Elwahab A. Ameliorative effect of taurine-chloramine in azathioprine-induced testicular damage; a deeper insight into the mechanism of protection. BMC Complement Altern Med. 2018 Sep; 18(1): 255. doi: 10.1186/s12906-018-2272-z.
29. Rezvanfar MA, Rezvanfar MA, Shahverdi AR, Ahmadi A, Baeeri M, Mohammadirad A, et al. Protection of cisplatin-induced spermatotoxicity, DNA damage and chromatin abnormality by selenium nano-particles. Toxicol Appl Pharmacol. 2013 Feb; 266(3): 356-65. doi: 10.1016/j.taap.2012.11.025
30. Garrido N, Meseguer M, Simon C, Pellicer A, Remohi J. Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility. Asian J Androl. 2004 Mar; 6(1): 59-65.
31. Agarwal A, Makker K, Sharma R. Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol. 2008 Jan; 59(1): 2-11. doi: 10.1111/j.1600-0897.2007.00559.x
32. Attia SM, Ahmad SF, Okash RM, Bakheet SA. Dominant lethal effects of nocodazole in germ cells of male mice. Food Chem Toxicol. 2015 Mar; 77:101-4. doi: 10.1016/j.fct.2015.01.004
33. Oshio S, Ozaki S, Ohkawa I, Tajima T, Kaneko S, Mohri H. Mecobalamin promotes mouse sperm maturation. Andrologia. 1989 Mar-Apr; 21(2): 167-73.
34. Zhou DX, Qiu SD, Zhang J, Tian H, Wang HX. The protective effect of vitamin E against oxidative damage caused by formaldehyde in the testes of adult rats. Asian J Androl. 2006 Sep; 8(5): 584-88. doi: 10.1111/j.1745-7262.2006.00198.x
35. Codrington AM, Hales BF, Robaire B. Exposure of male rats to cyclophosphamide alters the chromatin structure and basic proteome in spermatozoa. Hum Reprod. 2007 May; 22(5): 1431-42. doi: 10.1093/humrep/dem002
36. Cabral REL, Mendes TB, Vendramini V, Miraglia SM. Carnitine partially improves oxidative stress, acrosome integrity, and reproductive competence in doxorubicin-treated rats. Andrology. 2018 Jan; 6(1): 236-46. doi: 10.1111/andr.12426
37. Ghezzi M, Berretta M, Bottacin A, Palego P, Sartini B, Cosci I, et al. Impact of Bep or Carboplatin Chemotherapy on Testicular Function and Sperm Nucleus of Subjects with Testicular Germ Cell Tumor. Front Pharmacol. 2016 May; 7: 122. doi: 10.3389/fphar.2016.00122
38. Tavalaee M, Razavi S, Nasr-Esfahani MH. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil Steril. 2009 Apr; 91(4): 1119-26. doi: 10.1016/j.fertnstert.2008.01.063
39. Moustafa MH, Sharma RK, Thornton J, Mascha E, Abdel-Hafez MA, Thomas AJ Jr, et al. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum Reprod. 2004 Jan; 19(1): 129-38. doi: 10.1093/humrep/deh024
40. Guérin P, El Mouatassim S, Ménézo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update. 2001 Mar-Apr; 7(2): 175-89. doi: 10.1093/humupd/7.2.175
41. Raez LE, Kobina S, Santos ES. Oxaliplatin in first-line therapy for advanced non-small-cell lung cancer. Clin Lung Cancer. 2010 Jan; 11(1): 18-24. doi: 10.3816/CLC.2010.n.003
42. Stordal B, Pavlakis N, Davey R. Oxaliplatin for the treatment of cisplatin-resistant cancer: a systematic review. Cancer Treat Rev. 2007 Jun; 33(4): 347-57. doi: 10.1016/j.ctrv.2007.01.009
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Dahmardeh M, Sadeghinezhad J, Tootian Z, Salehnia M. Effect of Oxaliplatin on sperm parameters of 60 days old offspring during pre-pregnancy, pregnancy and lactation period in mice. J Gorgan Univ Med Sci 2020; 22 (1) :50-56
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Volume 22, Issue 1 (3-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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