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:: Volume 20, Issue 3 (10-2018) ::
J Gorgan Univ Med Sci 2018, 20(3): 19-23 Back to browse issues page
BMP 2 and BMP 4 genes expression in the development of the embryo heart in induced gestational diabetes of C57BL/6 mice
Fatemeh Ghasemzadeh1 , Masoud Golalipour2 , Kamran Haidari3 , Zahra Nazari4 , Mohamad Jafar Golalipour * 5
1- M.Sc Student in Anatomy, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
2- Assistant Professor, Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3- Associate Professor, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
4- Ph.D in Cell and Developmental Biology, Gorgan Congenital Malformations Research Center, Golestan University of Medical Sciences, Gorgan, Iran
5- Professor, Gorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran. mjgolalipour@yahoo.com , mjgolalipour@yahoo.com
Abstract:   (9359 Views)
Background and Objective: Gestational diabetes mellitus (GDM) is usually a disease caused by inadequate insulin production in pregnant women. GDM induces abnormal fetal growth.This study was done to evaluate the BMP2 and BMP4 genes expression in the development of the embryos heart in induced gestational diabetes of C57BL/6 mice.
Methods: In this experimental study, 8-week old adult C57BL/6 mice were randomly divided into diabetic and control groups. After mating of animals, the dams in diabetic group were received a single dose of 150 mg/kg/bw of streptozotocin on gestational day 1 of pregnancy, intrapereatonally. After 11.5 days of pregnancy, the embryos of both groups were extracted and heart tissue was extracted. RNA total tissue of the heart was extracted by trazole. After extracting RNA, expression of BMP2 and BMP4 genes in the heart of both groups was estimated by Real-time PCR.
Results: There was no singnificant diference in expression of BMP2 and BMP4 genes in the heart of 11.5 days of embryos in gestational diabetes mellitus group and control group.
Conclusion: Gestational diabetes mellitus had no effect on the expression of BMP2 and BMP4 genes in the development of the embryos heart.
Keywords: Gestational diabetes mellitus, Heart development, BMP2 gene, BMP4 gene, Mouse
Full-Text [PDF 263 kb] [English Abstract]   (12100 Downloads) |   |   Abstract (HTML)  (273 Views)  
Type of Study: Original Articles | Subject: Embryology
* Corresponding Author Address: Professor, Gorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran. mjgolalipour@yahoo.com
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Ghasemzadeh F, Golalipour M, Haidari K, Nazari Z, Golalipour M J. BMP 2 and BMP 4 genes expression in the development of the embryo heart in induced gestational diabetes of C57BL/6 mice. J Gorgan Univ Med Sci 2018; 20 (3) :19-23
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Volume 20, Issue 3 (10-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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