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:: Volume 20, Issue 3 (10-2018) ::
J Gorgan Univ Med Sci 2018, 20(3): 19-23 Back to browse issues page
BMP 2 and BMP 4 genes expression in the development of the embryo heart in induced gestational diabetes of C57BL/6 mice
Fatemeh Ghasemzadeh1 , Masoud Golalipour2 , Kamran Haidari3 , Zahra Nazari4 , Mohamad Jafar Golalipour * 5
1- M.Sc Student in Anatomy, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
2- Assistant Professor, Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3- Associate Professor, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
4- Ph.D in Cell and Developmental Biology, Gorgan Congenital Malformations Research Center, Golestan University of Medical Sciences, Gorgan, Iran
5- Professor, Gorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran. mjgolalipour@yahoo.com , mjgolalipour@yahoo.com
Abstract:   (11010 Views)
Background and Objective: Gestational diabetes mellitus (GDM) is usually a disease caused by inadequate insulin production in pregnant women. GDM induces abnormal fetal growth.This study was done to evaluate the BMP2 and BMP4 genes expression in the development of the embryos heart in induced gestational diabetes of C57BL/6 mice.
Methods: In this experimental study, 8-week old adult C57BL/6 mice were randomly divided into diabetic and control groups. After mating of animals, the dams in diabetic group were received a single dose of 150 mg/kg/bw of streptozotocin on gestational day 1 of pregnancy, intrapereatonally. After 11.5 days of pregnancy, the embryos of both groups were extracted and heart tissue was extracted. RNA total tissue of the heart was extracted by trazole. After extracting RNA, expression of BMP2 and BMP4 genes in the heart of both groups was estimated by Real-time PCR.
Results: There was no singnificant diference in expression of BMP2 and BMP4 genes in the heart of 11.5 days of embryos in gestational diabetes mellitus group and control group.
Conclusion: Gestational diabetes mellitus had no effect on the expression of BMP2 and BMP4 genes in the development of the embryos heart.
Keywords: Gestational diabetes mellitus, Heart development, BMP2 gene, BMP4 gene, Mouse
Full-Text [PDF 263 kb] [English Abstract]   (16315 Downloads) |   |   Abstract (HTML)  (410 Views)  
Type of Study: Original Articles | Subject: Embryology
* Corresponding Author Address: Professor, Gorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran. mjgolalipour@yahoo.com
References
1. Li PY, Hsu CC, Yin MC, Kuo YH, Tang FY, Chao CY. Protective Effects of red guava on inflammation and oxidative stress in Streptozotocin-induced diabetic mice. Molecules. 2015 Dec; 20(12): 22341-50. doi:10.3390/molecules201219831
2. Khoshnniat Nikoo M, Abbaszadeh Ahranjani S, Larijani B. A review on the prevalence of gestational diabetes mellitus (GDM) in different regions of Iran. Iranian Journal of Diabetes and Lipid Disorders. 2009; 8: 47-56.
3. American Diabetes Association. (2) Classification and diagnosis of diabetes. Diabetes Care. 2015 Jan; 38 Suppl: S8-S16. doi:10.2337/dc15-S005
4. Anastasiou E, Lekakis JP, Alevizaki M, Papamichael CM, Megas J, Souvatzoglou A, et al. Impaired endothelium-dependent vasodilatation in women with previous gestational diabetes. Diabetes Care. 1998 Dec; 21(12): 2111-15.
5. Peters SA, Huxley RR, Woodward M. Sex differences in body anthropometry and composition in individuals with and without diabetes in the UK Biobank. BMJ Open. 2016 Jan; 6(1): e010007. doi:10.1136/bmjopen-2015-010007
6. Moazzen H, Lu X, Ma NL, Velenosi TJ, Urquhart BL, Wisse LJ, et al. N-Acetylcysteine prevents congenital heart defects induced by pregestational diabetes. Cardiovasc Diabetol. 2014 Feb; 13: 46. doi:10.1186/1475-2840-13-46
7. Wang J, Greene SB, Martin JF. BMP signaling in congenital heart disease: new developments and future directions. Birth Defects Res A Clin Mol Teratol. 2011 Jun; 91(6): 441-48. doi:10.1002/bdra.20785
8. Miyazono K, Kamiya Y, Morikawa M. Bone morphogenetic protein receptors and signal transduction. J Biochem. 2010 Jan; 147(1): 35-51. doi:10.1093/jb/mvp148
9. Bandyopadhyay A, Yadav PS, Prashar P. BMP signaling in development and diseases: a pharmacological perspective. Biochem Pharmacol. 2013 Apr; 85(7): 857-64. doi:10.1016/j.bcp.2013.01.004
10. Wang RN, Green J, Wang Z, Deng Y, Qiao M, Peabody M, et al. Bone morphogenetic protein (BMP) signaling in development and human diseases. Genes Dis. 2014 Sep; 1(1): 87-105.
11. McCulley DJ, Kang JO, Martin JF, Black BL. BMP4 is required in the anterior heart field and its derivatives for endocardial cushion remodeling, outflow tract septation, and semilunar valve development. Dev Dyn. 2008 Nov; 237(11): 3200–209. doi:10.1002/dvdy.21743
12. Somi S, Buffing AA, Moorman AF, Van Den Hoff MJ. Dynamic patterns of expression of BMP isoforms 2, 4, 5, 6, and 7 during chicken heart development. Anat Rec A Discov Mol Cell Evol Biol. 2004 Jul; 279(1): 636-51.
13. Golalipour MJ, Ghafari S, Moharreri A. [Gestational diabetes reduces motor neurons of spinal cord in 4, 8 and 12 weeks rat offspring]. J Gorgan Univ Med Sci. 2014; 16(1): 29-34. [Article in Persian]
14. Vafaeipour Z, Shokrzadeh M, Jahani M, Shaki F. [Protective effect of Nanoceria against Streptozotocin induced mitochondrial dysfunction in embryo of diabetic mice]. J Mazandaran Univ Med Sci. 2015; 25(3): 109-20. [Article in Persian]
15. Lister R, Einstein F, Chamberlain A, Dar P, Bernstein P, Zhou B. 269: Streptozotocin dosing for the induction of fetal cardiac dysmorphology associated with maternal hyperglycemia. American J Obstet Gynecol. 2012; 206(1): S131. https://doi.org/10.1016/j.ajog.2011.10.287
16. Damasceno DC, Sinzato YK, Bueno A, Netto AO, Dallaqua B, Gallego FQ, et al. Mild diabetes models and their maternal-fetal repercussions. J Diabetes Res. 2013; 2013: 473575. doi:10.1155/2013/473575
17. Øyen N, Diaz LJ, Leirgul E, Boyd HA, Priest J, Mathiesen ER, et al. Prepregnancy diabetes and offspring risk of congenital heart disease: a nationwide cohort study. Circulation. 2016 Jun; 133(23): 2243-53. doi:10.1161/CIRCULATIONAHA.115.017465
18. Moazzen H. Pregestational diabetes and congenital heart defects: role of reactive oxygen species. Dissertation, Physiology. The University of Western Ontario. 2014.
19. Luo JY, Zhang Y, Wang L, Huang Y. Regulators and effectors of bone morphogenetic protein signalling in the cardiovascular system. J Physiol. 2015 Jul; 593(14): 2995-3011. doi:10.1113/JP270207
20. Corrigan N, Brazil DP, McAuliffe F. Fetal cardiac effects of maternal hyperglycemia during pregnancy. Birth Defects Res A Clin Mol Teratol. 2009 Jun; 85(6): 523-30. doi:10.1002/bdra.20567
21. Wang G, Huang WQ, Cui SD, Li S, Wang XY, Li Y, et al. Autophagy is involved in high glucose-induced heart tube malformation. Cell Cycle. 2015; 14(5): 772-83. doi:10.1080/15384101.2014.1000170
22. Luo JY, Zhang Y, Wang L, Huang Y. Regulators and effectors of bone morphogenetic protein signalling in the cardiovascular system. J Physiol. 2015 Jul; 593(14): 2995-3011. doi:10.1113/JP270207
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Ghasemzadeh F, Golalipour M, Haidari K, Nazari Z, Golalipour M J. BMP 2 and BMP 4 genes expression in the development of the embryo heart in induced gestational diabetes of C57BL/6 mice. J Gorgan Univ Med Sci 2018; 20 (3) :19-23
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Volume 20, Issue 3 (10-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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