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:: Volume 20, Issue 3 (10-2018) ::
J Gorgan Univ Med Sci 2018, 20(3): 82-89 Back to browse issues page
Antibacterial effect of Berberin and Curcumin complexes on Escherichia coli and Bacillus pedillus bacteria and comparison of their cytotoxicity on the cell line of bladder and stomach cancer cells
Zahra Alkhamis1 , Mahdiye Mostafavi2 , Leila Hassani * 3, Fakhrossadat Mohammadi4 , Khosro Mohammadi5
1- (First Author) M.A in Biochemistry, Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences, Zanjan, Iran
2- (First Author) M.A in Biophysics, Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences and Ph.D Candidate in Biochemistry, Department of Biological Sciences, Tarbiat Modares University, Tehran, Iran
3- Associate Professor, Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences, Zanjan, Iran. hasani@iasbs.ac.ir , hasani@iasbs.ac.ir
4- Assistant Professor, Department of Chemistry, Institute for Advanced Studies in Basic Sciences, Zanjan, Iran
5- Associate Professor, Department of Chemistry, Faculty of Sciences, Persian Gulf University, Bushehr, Iran
Abstract:   (11838 Views)
Background and Objective: Curcumin is a combination of active polyphenol from the Curcuma Langa plant, which has extensive biological activities including effects anti-inflammatory, anti-bacterial and cytotoxic markers for multiple cancer cells. Berberine is an alkaloied isokinolin that is present in berberine and suppresses the growth of many tumor cells. This study was designed to determine the antibacterial effect of berberine and indium curcumin and indium diastile curcumin complexes against E-coli and Bacillus pumilus and comparison of their cytotoxicity on the cell lines of the bladder and stomach cancer cells.
Methods: In this descriptive-analytic study, antimicrobial activity and cytotoxicity effect of berberine and indium curcumin and indium diastile curcumin complexes was investigated by MTT and dilution test method respectively. E-coli [BL21 (DE 3)], Bacillus pumilus (PTCC 1529), cell lines of bladder (5637) and stomach (AGS) were evaluated.
Results: The minimum inhibitory concentration (MIC) of berberin for E-coli was determined 5 mM. At 100 micromolar concentration of berberine approximately 100% of the bladder cancer cells have disappeared. Cytotoxic effect of curcumin complexes on two bladder and stomach cancer cell lines showed that both complexes have different inhibitory effects on cell line life. Cytotoxicity of 20μM indium curcumin and indium diastile curcumin complexes for bladder cancer cells were 58% and 55%, respectively, and for stomach cancer cells were 61% and 34 %, respectively. Antibacterial activity of complexes against Bacillus pumilus and E-coli showed that none of the complexes has antimicrobial effect against Bacillus Pamilus, but both complexes inhibited the growth of E-coli bacteria. The bacteria population in the presence of indium curcumin and indium diastile curcumin complexes was reduced to 40% and 24%, respectively.
Conclusion: This study indicated that indium complexes of curcumin and diacetyl curcumin have a potential for anticancer and antibacterial therapy. Furthermore, berberine as an alkaloid has anticancer and antibacterial activity.
Keywords: Curcumin, Berberine, Anticancer, Antibacterial, Cytotoxicity, E-coli, Bacillus pumilus
Full-Text [PDF 407 kb] [English Abstract]   (16467 Downloads) |   |   Abstract (HTML)  (430 Views)  
Type of Study: Original Articles | Subject: Microbiology
* Corresponding Author Address: Associate Professor, Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences, Zanjan, Iran. hasani@iasbs.ac.ir


 
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Alkhamis Z, Mostafavi M, Hassani L, Mohammadi F, Mohammadi K. Antibacterial effect of Berberin and Curcumin complexes on Escherichia coli and Bacillus pedillus bacteria and comparison of their cytotoxicity on the cell line of bladder and stomach cancer cells. J Gorgan Univ Med Sci 2018; 20 (3) :82-89
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Volume 20, Issue 3 (10-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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