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:: Volume 21, Issue 1 (3-2019) ::
J Gorgan Univ Med Sci 2019, 21(1): 38-45 Back to browse issues page
Effect of diazinon on functional tests and liver tissue alterations in adult rats
Seyyed Ali Haghightjoo1 , Mehrdad Shariati * 2, Mokhtar Mokhtari3 , Davood Moghadamnia4
1- M.Sc in Animal Physiology, Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
2- Associate Professor, Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran , mehrdadshariati48@gmail.com
3- Professor, Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
4- Ph.D in Animal Physiology, Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University, Shiraz, Iran
Abstract:   (9529 Views)
Background and Objective: The diazinon insecticide is a strong acetylcholinesterase controller at the nerve terminals. This study was conducted to determine the effect of diazinon on functional tests and tissue alterations in rat liver.
Methods: In this experimental study, 40 adult male Wistar rats were allocated into 5 groups including the first, second and third experimental groups were received 50, 100 and 150 mg/kg/bw of diazinon, respectively, orally during 21 days. The control group did not receive any medication and the sham group received solvent. At the end of study, blood samples were obtained from animals and Alanine transaminase (ALT), Aspartate transaminase (AST), Alkaline phosphatase (ALP) hepatic enzymes, total protein, albumin and bilirubin were measured. After anesthesia the liver of animals were removed and evaluated by hematoxylin eosin histological staining methods.
Results: The level of AST and ALT enzymes in the experimental groups receiving 100 and 150 mg/kg/bw doses of diazinon significantly increased in compared to the control group (p<0.05). The level of ALP of animals in all groups receiving diazinon was significantly higher than the control group (P<0.05). Total protein level significantly reduced in all groups receiving diazinon compared to the control group (P<0.05). The level of albumin in the experimental group receiving 150 mg/kg/bw dose of diazinon significantly reduced in comparision with controls. In the experimental groups, tissue samples showed more necrosis with increasing doses of diazinon.
Conclusion: Diazinon increases level of liver enzymes and decreased total protein and albumin level and causes the histological alterations in rat liver.
Keywords: Diazinon, Liver Enzymes, Liver Necrosis, Rat
Full-Text [PDF 268 kb]   (15073 Downloads)    
Type of Study: Original Articles | Subject: Physiology - Pharmacology
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Haghightjoo S A, Shariati M, Mokhtari M, Moghadamnia D. Effect of diazinon on functional tests and liver tissue alterations in adult rats. J Gorgan Univ Med Sci 2019; 21 (1) :38-45
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Volume 21, Issue 1 (3-2019) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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