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:: Volume 19, Issue 2 (7-2017) ::
J Gorgan Univ Med Sci 2017, 19(2): 91-97 Back to browse issues page
Detection of phospholipase and type IV pili genes in clinical isolates of multidrug-resistant Pseudomonas aeruginosa
A Monazami1 , F Haghi * 2
1- M.Sc Student in Microbiology, Department of Microbiology, Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2- Associate Professor, Department of Microbiology and Virology, Zanjan University of Medical Sciences, Zanjan, Iran , haghi@zums.ac.ir
Abstract:   (10929 Views)

Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen with numerous virulence factors such as phospholipase and type IV pili. The emergence of multidrug resistant Pseudomonas aeruginosa has become a serious public health threat worldwide. This study was done to determine the frequency of plcH, plcN, pilA and pilB genes in multi-drug resistant Pseudomonas aeruginosa isolated from clinical samples.

Methods: In this cross-sectional study, 93 isolates of Pseudomonas aeruginosa collected from different clinical samples from hospitals of Zanjan, Iran during 2013-14. After identification of isolates by biochemical tests, antibiotic susceptibility testing (Kirby-Bauer) was performed according to CLSI guidelines. Total DNA extracted and PCR was done to detect of plcH, plcN, pilA and pilB genes.

Results: Among 93 of Pseudomonas aeruginosa isolates, the highest antibiotic resistance related to Erythromycin and Cefoxitin (95.6%) and the lowest resistance related to Amikacin (26.8%). 80.6% of isolates were multidrug resistant (MDR). Out of 75 MDR isolates, the frequency of plcH, plcN, pilA and pilB genes was 97.4%, 49.3%, 26.6% and 17.3%, respectively.

Conclusion: According to high frequency of phospholipase C gene (plcH) in MDR Pseudomonas aeruginosa isolates which isolated from different clinical samples, presumably this virulence factor plays an important role in pathogenesis of this bacterium.
Keywords: Pseudomonas aeruginosa, Antibiotic resistance, Phospholipase C, Type IV Pili
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Type of Study: Original Articles | Subject: Microbiology
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Monazami A, Haghi F. Detection of phospholipase and type IV pili genes in clinical isolates of multidrug-resistant Pseudomonas aeruginosa . J Gorgan Univ Med Sci 2017; 19 (2) :91-97
URL: http://goums.ac.ir/journal/article-1-3083-en.html
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Volume 19, Issue 2 (7-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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