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:: Volume 19, Issue 2 (7-2017) ::
J Gorgan Univ Med Sci 2017, 19(2): 17-24 Back to browse issues page
Effect of Nigella sativa oil against Bisphenol A induced toxicity on the tissue of male NMRI mice kidney : A stereological study
M Soleimani Mehranjani * 1, E Safari Senejani2 , S Naderi Noreini3
1- Professor, Department of Biology, Faculty of Science, Arak University, Arak,Iran , m-soleimani@araku.ac.ir
2- M.Sc in Developmental Biology
3- M.Sc in Developmental Biology, Infertility Center of Omid Royan, Academic Center for Education, Culture & Research (ACECR), Arak Branch, Iran
Abstract:   (10200 Views)

Background and Objective: Bisphenol A (BPA) is an endocrine disruptor chemical and as an environmental pollutant is able to generate free radicals causing tissue damage. This study was done to investigate the effect of Nigella sativa oil against BPA induced toxicity on the tissue of male NMRI mice kidney by stereological method.

Methods: In this experimental study 24 adult male NMRI mice (32±3 g) were randomly allocated into control, BPA (200 mg/kg/day), BPA (200 mg/kg/day) plus Nigella sativa oil (5 ml/kg/day) and Nigella sativa oil (5 ml/kg/day) groups and treated for 5 weeks, orally. At the end, animals were sacrificed, their left kidneys were removed, fixed, sectioned, processed and stained with Heidenhain' azan staining method. Then, the kidney tissue sections were evaluated using stereological method and serum malondialdehyde (MDA) level was also measured.

Results: The total weight and volume of kidney, volume of cortex, volume of proximal and distal tubules and volume of their lumen, volume of interstitial tissue, volume of glomeruli, tuft, as well as serum MDA level significantly increased in BPA treated group compared to the controls (P<0.05). These parameters were significantly reduced in BPA plus Nigella sativa oil group compared to BPA ones (P<0.05).

Conclusion: This study revealed that Nigella sativa oil can reduce the oxidative stress toxicity induced by BPA in the mice renal tissue.
Keywords: Kidney, Bisphenol A, Nigella sativa oil, Stereology, Mouse
Full-Text [PDF 406 kb] [English Abstract]   (15116 Downloads) |   |   Abstract (HTML)  (1299 Views)  
Type of Study: Original Articles | Subject: Physiology - Pharmacology
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Soleimani Mehranjani M, Safari Senejani E, Naderi Noreini S. Effect of Nigella sativa oil against Bisphenol A induced toxicity on the tissue of male NMRI mice kidney : A stereological study. J Gorgan Univ Med Sci 2017; 19 (2) :17-24
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Volume 19, Issue 2 (7-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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