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:: Volume 18, Issue 1 (3-2016) ::
J Gorgan Univ Med Sci 2016, 18(1): 30-38 Back to browse issues page
Effect of green tea extract (Camellia sinensis) on kidney toxicity induced by sodium arsenite: a stereological study
Shariatzadeh SMA1 , Soleimani Mehranjani M2 , Shahmohammadi R * 3, Naderi Noreini S4
1- Professor, Department of Biology, Faculty of Science, Arak University, Arak, Iran
2- Associate Professor, Department of Biology, Faculty of Science, Arak University, Arak, Iran
3- M.Sc Student of Developmental Cell Biology, Department of Biology, Faculty of Science, Arak University, Arak, Iran , rj.shahmohammadi@gmail.com
4- M.Sc in Developmental Cell Biology, Department of Biology, Faculty of Science, Arak University, Arak, Iran
Abstract:   (12797 Views)

Background and Objective: Sodium Arsenite is an environmental pollutant which can generate free radicals causing tissue damage. This study was done to evaluate the effect of Green Tea (GTE), as a strong antioxidant, on kidney tissue in mice treated with Sodium Arsenite. Methods: In this experimental study 24 adult male NMRI mice were randomly allocated into four groups including: control, GTE (100mg/kg/day), Sodium Arsenite (5mg/kg/day) and Sodium Arsenite + GTE, for 34 days, orally. Animals were scarified and left kidney was taken out, fixed, sectioned, processed and stained using Heidenhain'azan method. Using stereological technique the total volume of kidney, volume of cortex, medulla, proximal and distal tubule, renal corpuscle, gelomerelus, tuft and capillary, membrane and space of Bowman's capsule and length of proximal and distal tubule were determined. Creatinine, BUN and MDA serum samples were measured. Results: The mean of total volume of cortex, proximal tubule, distal tubule, renal corpuscle and gelomerolus, taft, Bowman's capsule space, size of epithelium and lumen of proximal and distal tubule were significantly reduced in Sodium Arsenite group compared to control (P<0.05). These parameters were significantly increased in the Sodium Arsenite + GTE group in comparison with Sodium Arsenite group (P<0.05). The creatinine, Blood urea nitrogen (BUN) and MDA were significantly increased in the Sodium Arsenite group in compared to the control group (P<0.05). These parameters were significantly reduced in the Sodium Arsenite + GTE group in comparison with Sodium Arsenite group (P<0.05). Conclusion: Green tea has a protective role in Sodium Arsenite induced nephrotoxicity.
Keywords: Kidney, Green Tea, Sodium Arsenite, Stereology, Mouse
Full-Text [PDF 287 kb] [English Abstract]   (13413 Downloads) |   |   Abstract (HTML)  (252 Views)  
Type of Study: Original Articles | Subject: Physiology - Pharmacology
References
1. Pradosh R, Anupama S. Metabolism and toxicity of arsenic: a human carcinogen. Current Science. 2002; 82(1): 38-45.
2. Aronson SM. Arsenic and old myths. R I Med. 1994 Jul; 77(7):233-4.
3. Sprando RL, Collins TF, Black T, Olejnik N, Ramos-Valle M, Ruggles D. Acute toxicity of sodium arsenite in a complex food matrix. Food Chem Toxicol. 2007 Sep;45(9):1606-13.
4. Mazumder DN. Effect of chronic intake of arsenic-contaminated water on liver. Toxicol Appl Pharmacol. 2005 Aug; 206(2):169-75.
5. Liu L, Trimarchi JR, Navarro P, Blasco MA, Keefe DL. Oxidative stress contributes to arsenic-induced telomere attrition, chromosome instability, and apoptosis. J Biol Chem. 2003 Aug; 278(34):31998-2004.
6. Modi M, Kaul RK, Kannan GM, Flora SJ. Co-administration of zinc and n-acetylcysteine prevents arsenic-induced tissue oxidative stress in male rats. J Trace Elem Med Biol. 2006;20(3):197-204.
7. Kimura A, Ishida Y, Hayashi T, Wada T, Yokoyama H, Sugaya T, et al. Interferon-gamma plays protective roles in sodium arsenite-induced renal injury by up-regulating intrarenal multidrug resistance-associated protein 1 expression. Am J Pathol. 2006 Oct; 169(4):1118-28.
8. Lewis DR, Southwick JW, Oulet-Helstrom R, Rench J, Calderon RL. Drinking water arsenic in Utah: a cohort mortality study. Environ Health Perspect. 1999 May; 107(5):359-65.
9. Roy S, Bhattacharya S. Arsenic-induced histopathology and synthesis of stress proteins in liver and kidney of Channa punctatus. Ecotoxicol Environ Saf. 2006 Oct;65(2):218-29.
10. Chen D, Wan SB, Yang H, Yuan J, Chan TH, Dou QP. EGCG, green tea polyphenols and their synthetic analogs and prodrugs for human cancer prevention and treatment. Adv Clin Chem. 2011;53:155-77.
11. Chacko SM, Thambi PT, Kuttan R, Nishigak I. Beneficial effects of green tea: A literature review. Chin Med. 2010; 5:13. doi:10.1186/1749-8546-5-13
12. Ostrowska J, Skrzydlewska E. The comparison of effect of catechins and green tea extract on oxidative modification of LDL in vitro. Adv Med Sci. 2006;51:298-303.
13. Lung HL, Ip WK, Wong CK, Mak NK, Chen ZY, Leung KN. Anti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell line. Life Sci. 2002 Dec; 72(3):257-68.
14. Yousef MI, El-Demerdash FM, Radwan FM. Sodium arsenite induced biochemical perturbations in rats: ameliorating effect of curcumin. Food Chem Toxicol. 2008 Nov;46(11):3506-11. doi:10.1016/j.fct.2008.08.031
15. Awoniyi DO, Aboua YG, Marnewick JL, du Plesis SS, Brooks NL. Protective effects of rooibos (Aspalathus linearis), green tea (Camellia sinensis) and commercial supplements on testicular tissue of oxidative stressinduced rats. African Journal of Biotechnology. 2011; 10(75): 17317-22. http://dx.doi.org/10.5897/AJB11.2210
16. Chinoy NJ, Shah SD. Beneficial effects of some antidotes in fluoride and arsenic induced toxicity in kidney of mice. Fluoride Journal. 2004; 37(3):151-61.
17. Mandarim-de-Lacerda CA. Stereological tools in biomedical research. An Acad Bras Cienc. 2003 Dec;75(4):469-86.
18. Soleimani Mehranjani M, Shariatzadeh SMA. [Quantitative study of the histological effects of sodium arsenite on kidney structure in rats]. J Arak Uni Med Sci. 2008; 10(4):57-63. [Article in Persian]
19. Hoseini L, Roozbeh J, Sagheb M, Karbalay-Doust S, Noorafshan A. Nandrolone decanoate increases the volume but not the length of the proximal and distal convoluted tubules of the mouse kidney. Micron. 2009 Feb; 40(2):226-30. doi:10.1016/j.micron.2008.08.004
20. Pazvant G, Sahin B, Kahvecioglu K, Gunes H, Gezerince N, Bacinoglu D. The volume fraction method for the evaluation of kidney: A stereological study. Ankara Univ Vet Fak Derg. 2009; 56: 233-239.
21. Nyengaard JR, Flyvbjerg A, Rasch R. The impact of renal growth, regression and re-growth in experimental diabetes mellitus on number and size of proximal and distal tubular cells in the rat kidney. Diabetologia. 1993; 36: 1126-31.
22. Dezfoolian AAR, Panahi M, Feizi F. Stereological evaluation of renal glomeruli in offspring of diabetic female rats. Cell Journal (Yakhteh). 2009; 11(1): 17-22.
23. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52:302-10.
24. Shila S, Subathra M, Devi MA, Panneerselvam C. Arsenic intoxication-induced reduction of glutathione level and of the activity of related enzymes in rat brain regions: reversal by DL-alpha-lipoic acid. Arch Toxicol. 2005 Mar;79(3):140-6.
25. Gupta R, Flora SJ. Protective value of Aloe vera against some toxic effects of arsenic in rats. Phytother Res. 2005 Jan;19(1):23-8.
26. Sohini, Rana SV. Protective effect of ascorbic acid against oxidative stress induced by inorganic arsenic in liver and kidney of rat. Indian J Exp Biol. 2007 Apr;45(4):371-5.
27. Silva FG. Chemical-induced nephropathy: a review of the renal tubulointerstitial lesions in humans. Toxicol Pathol. 2004 Jul-Aug; 32 (Suppl 2): 71-84.
28. Tsukamoto H, Parker HR, Gribble DH, Mariassy A, Peoples SA. Nephrotoxicity of sodium arsenate in dogs. Am J Vet Res. 1983 Dec;44(12):2324-30.
29. Flora SJ, Mittal M, Mehta A. Heavy metal induced oxidative stress & its possible reversal by chelation therapy. Indian J Med Res. 2008 Oct;128(4):501-23.
30. Eguia L, Materson BJ. Acetaminophen-related acute renal failure without fulminant liver failure. Pharmacotherapy. 1997 Mar-Apr; 17(2):363-70.
31. Noman AS, Dilruba S, Mohanto NC, Rahman L, Khatun Z, Riad W, et al. Arsenic-induced Histological Alterations in Various Organs of Mice. J Cytol Histol. 2015 May;6(3). pii: 323.
32. Mescher A. [Basic Histology]. Translate by: Naderan M, Naderan M, Noori Moogehi SMH. 1st. Tehran: Teimourzadeh Publications. 2011.
33. Chang SI, Jin B, Youn P, Park C, Park JD, Ryu DY. Arsenic-induced toxicity and the protective role of ascorbic acid in mouse testis. Toxicol Appl Pharmacol. 2007 Jan; 218(2):196-203.
34. Saad SY, Alkharfy KM, Arafah MM. Cardiotoxic effects of arsenic trioxide/imatinib mesilate combination in rats. J Pharm Pharmacol. 2006 Apr;58(4):567-73.
35. Agarwal A, Prabakaran SA. Mechanism, measurement, and prevention of oxidative stress in male reproductive physiology. Indian J Exp Biol. 2005 Nov;43(11):963-74.
36. Halliwell B. Antioxidants and human disease: a general introduction. Nutr Rev. 1997 Jan; 55(1 Pt 2):S44-9.
37. Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes. 1991 Apr;40(4):405-12.
38. Cuddihy SL, Parker A, Harwood DT, Vissers MC, Winterbourn CC. Ascorbate interacts with reduced glutathione to scavenge phenoxyl radicals in HL60 cells. Free Radic Biol Med. 2008 Apr; 44(8):1637-44. doi:10.1016/j.freeradbiomed.2008.01.021
39. Akter KF, Owens G, Davey DE, Naidu R. Arsenic speciation and toxicity in biological systems. Rev Environ Contam Toxicol. 2005;184:97-149.
40. Bosy A Abd El-Motelp. Ameliorative effect of n-acetyl cysteine on sodium arsenite induced toxicity and oxidative stress in mice. World Journal of Pharmaceutical Research (WJPR). 2014; 3(2): 1746-59.
41. Tandan N, Roy M, Roy S. Ameliorative potential of psidium guajava on hemato-biochemical alterations in arsenic-exposed Wistar rats. Toxicol Int. 2012 May-Aug; 19(2): 121-24. doi:10.4103/0971-6580.97199
42. Gad SB, Zaghloul DM. Beneficial effects of green tea extract on liver and kidney functions, ultrastructure, lipid profile and hematological parameters in aged male rats. Global Veterinaria. 2013; 11(2): 191-205. doi:10.5829/idosi.gv.2013.11.2.7472
43. Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009 Nov-Dec; 2(5): 270-78. doi:10.4161/oxim.2.5.9498
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Shariatzadeh SMA, Soleimani Mehranjani M, Shahmohammadi R, Naderi Noreini S. Effect of green tea extract (Camellia sinensis) on kidney toxicity induced by sodium arsenite: a stereological study. J Gorgan Univ Med Sci 2016; 18 (1) :30-38
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Volume 18, Issue 1 (3-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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