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:: Volume 18, Issue 1 (3-2016) ::
J Gorgan Univ Med Sci 2016, 18(1): 30-38 Back to browse issues page
Effect of green tea extract (Camellia sinensis) on kidney toxicity induced by sodium arsenite: a stereological study
Shariatzadeh SMA1 , Soleimani Mehranjani M2 , Shahmohammadi R * 3, Naderi Noreini S4
1- Professor, Department of Biology, Faculty of Science, Arak University, Arak, Iran
2- Associate Professor, Department of Biology, Faculty of Science, Arak University, Arak, Iran
3- M.Sc Student of Developmental Cell Biology, Department of Biology, Faculty of Science, Arak University, Arak, Iran , rj.shahmohammadi@gmail.com
4- M.Sc in Developmental Cell Biology, Department of Biology, Faculty of Science, Arak University, Arak, Iran
Abstract:   (13664 Views)

Background and Objective: Sodium Arsenite is an environmental pollutant which can generate free radicals causing tissue damage. This study was done to evaluate the effect of Green Tea (GTE), as a strong antioxidant, on kidney tissue in mice treated with Sodium Arsenite. Methods: In this experimental study 24 adult male NMRI mice were randomly allocated into four groups including: control, GTE (100mg/kg/day), Sodium Arsenite (5mg/kg/day) and Sodium Arsenite + GTE, for 34 days, orally. Animals were scarified and left kidney was taken out, fixed, sectioned, processed and stained using Heidenhain'azan method. Using stereological technique the total volume of kidney, volume of cortex, medulla, proximal and distal tubule, renal corpuscle, gelomerelus, tuft and capillary, membrane and space of Bowman's capsule and length of proximal and distal tubule were determined. Creatinine, BUN and MDA serum samples were measured. Results: The mean of total volume of cortex, proximal tubule, distal tubule, renal corpuscle and gelomerolus, taft, Bowman's capsule space, size of epithelium and lumen of proximal and distal tubule were significantly reduced in Sodium Arsenite group compared to control (P<0.05). These parameters were significantly increased in the Sodium Arsenite + GTE group in comparison with Sodium Arsenite group (P<0.05). The creatinine, Blood urea nitrogen (BUN) and MDA were significantly increased in the Sodium Arsenite group in compared to the control group (P<0.05). These parameters were significantly reduced in the Sodium Arsenite + GTE group in comparison with Sodium Arsenite group (P<0.05). Conclusion: Green tea has a protective role in Sodium Arsenite induced nephrotoxicity.
Keywords: Kidney, Green Tea, Sodium Arsenite, Stereology, Mouse
Full-Text [PDF 287 kb] [English Abstract]   (15108 Downloads) |   |   Abstract (HTML)  (328 Views)  
Type of Study: Original Articles | Subject: Physiology - Pharmacology
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Shariatzadeh SMA, Soleimani Mehranjani M, Shahmohammadi R, Naderi Noreini S. Effect of green tea extract (Camellia sinensis) on kidney toxicity induced by sodium arsenite: a stereological study. J Gorgan Univ Med Sci 2016; 18 (1) :30-38
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Volume 18, Issue 1 (3-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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