[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aims and Scope
Content Coverage
Conflict of Interest
Publication Ethics
Editorial Board::
Executive Members::
Instruction to Authors::
Peer Review::
Articles Archive::
Current Issue
All Issues
Indexing Databases::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 18, Issue 1 (3-2016) ::
J Gorgan Univ Med Sci 2016, 18(1): 23-29 Back to browse issues page
Effect of Diazinon on pituitary-gonadal axis and histological alteration of seminferous tubules in adult rat testis
Rahimi S * 1, Zamiri MJ2 , Shariati M3 , Changizi-Ashtiyani S4 , Moghadamnia D5 , Rahimi A6
1- M.Sc in Animal Physiology, Department of Biology, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran , somaye_biology@yahoo.com
2- M.Sc in Animal Physiology, Department of Biology, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran
3- Associate Professor, Department of Biology, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran
4- Associate Professor, Department of Physiology, Arak University of Medical Sciences, Arak, Iran
5- Ph.D Candidate in Animal Physiology, Islamic Azad University, Shiraz Branch, Shiraz, Iran
6- Doctor in Veterinary Medicine, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran
Abstract:   (10027 Views)

Background and Objective: Diazinon is an organophosphate insecticide. , which inhibits the enzyme acetylcholinesterase. This study was done to evaluate the effect of Diazinon on pituitary-gonadal axis and histological alteration of seminferous tubules in adult rat testis. Methods: In this experimental study, 40 adult male Wistar rats were randomly allocated into five groups including control, sham and experimental 1, 2 and 3. Animals in experimental group 1, 2 and 3 were received 50, 100 and 150 mg/kg/bw of diazinon for 28 days, orally, respectively. Animals in control group did not receive any substance. Animals in sham group were received an equivalent amount of normal saline. The animals were euthanized after 28 days and a blood sample was collected via heart puncture and testes were removed for histological studies. Results: Diazinon significantly reduced serum testosterone concentration, sertoli cell, leydig cell count, primary spermatocyte and spermatid (P<0.05). Diazinon had no significant effect on the body and testis weight in the experimental groups compared to controls. Conclusion: Diazinon reduces the concentration of testosterone and cells in seminferous tubule in adult rat.
Keywords: Diazinon, Testis tissue, Testosterone, Rat
Full-Text [PDF 237 kb] [English Abstract]   (16149 Downloads) |   |   Abstract (HTML)  (328 Views)  
Type of Study: Original Articles | Subject: Physiology - Pharmacology
References
1. Duysen EG, Cashman JR, Schopfer LM, Nachon F, Masson P, Lockridge O. Differential sensitivity of plasma carboxylesterase-null mice to parathion, chlorpyrifos and chlorpyrifos oxon, but not to diazinon, dichlorvos, diisopropylfluorophosphate, cresyl saligenin phosphate, cyclosarin thiocholine, tabun thiocholine, and carbofuran. Chem Biol Interact. 2012 Feb 5;195(3):189-98. doi:10.1016/j.cbi.2011.12.006.
2. Jones RR, Barone-Adesi F, Koutros S, Lerro CC, Blair A, Lubin J, et al. Incidence of solid tumours among pesticide applicators exposed to the organophosphate insecticide diazinon in the Agricultural health study: an updated analysis. Occup Environ Med. 2015 Jul;72(7):496-503. doi:10.1136/oemed-2014-102728
3. Goldner WS, Sandler DP, Yu F, Shostrom V, Hoppin JA, Kamel F, LeVan TD. Hypothyroidism and pesticide use among male private pesticide applicators in the agricultural health study. J Occup Environ Med. 2013 Oct; 55(10):1171-8. doi:10.1097/JOM.0b013e31829b290b
4. Slotkin TA, Ryde IT, Levin ED, Seidler FJ. Developmental neurotoxicity of low dose diazinon exposure of neonatal rats: effects on serotonin systems in adolescence and adulthood. Brain Res Bull. 2008 Mar; 75(5):640-7. doi:10.1016/j.brainresbull.2007.10.008
5. Slotkin TA, Seidler FJ. Developmental neurotoxicity of organophosphates targets cell cycle and apoptosis, revealed by transcriptional profiles in vivo and in vitro. Neurotoxicol Teratol. 2012 Mar;34(2):232-41. doi:10.1016/j.ntt.2011.12.001
6. Beane Freeman LE, Bonner MR, Blair A, Hoppin JA, Sandler DP, Lubin JH, et al. Cancer incidence among male pesticide applicators in the Agricultural Health Study cohort exposed to diazinon. Am J Epidemiol. 2005 Dec;162(11):1070-9.
7. Jayachandra S, D'Souza UJ. Pre- and postnatal toxicity of diazinon induces disruption of spermatogenetic cell line evidenced by increased testicular marker enzymes activities in rat offspring. J Environ Pathol Toxicol Oncol. 2013;32(1):73-90.
8. Fattahy E, Jorsaraei S, Parivar K, Moghaddamnia AA. [Influence of diazinon on spermatogenesis in mice]. Koomesh. 2007; 9(1): 75-82. [Article in Persian]
9. Guyton KZ, Loomis D, Grosse Y, El Ghissassi F, Benbrahim-Tallaa L, Guha N, et al. Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate. Lancet Oncol. 2015 May;16(5):490-1. doi:10.1016/S1470-2045(15)70134-8
10. Oh YJ, Jung YJ, Kang JW, Yoo YS. Investigation of the estrogenic activities of pesticides from Pal-dang reservoir by in vitro assay. Sci Total Environ. 2007 Dec; 388(1-3):8-15.
11. Woodward K ,‎ Marrs T,‎ Waters MD. Toxicological effects of veterinary medicinal products in humans. 1st. Cambridge: RSC Press. 2012; p: 181.
12. El-Demerdash FM, Nasr HM. Antioxidant effect of selenium on lipid peroxidation, hyperlipidemia and biochemical parameters in rats exposed to diazinon. J Trace Elem Med Biol. 2014 Jan; 28(1):89-93. doi:10.1016/j.jtemb.2013.10.001
13. Bisson M, Hontela A. Cytotoxic and endocrine-disrupting potential of atrazine, diazinon, endosulfan, and mancozeb in adrenocortical steroidogenic cells of rainbow trout exposed in vitro. Toxicol Appl Pharmacol. 2002 Apr; 180(2):110-7.
14. Mazzocchi G, Malendowicz LK, Gottardo L, Aragona F, Nussdorfer GG. Orexin A stimulates cortisol secretion from human adrenocortical cells through activation of the adenylate cyclase-dependent signaling cascade. J Clin Endocrinol Metab. 2001 Feb; 86(2):778-82.
15. Ahmed MA, Ahmed HI, El-Morsy EM. Melatonin protects against diazinon-induced neurobehavioral changes in rats. Neurochem Res. 2013 Oct; 38(10):2227-36. doi:10.1007/s11064-013-1134-9
16. Najafi GR, Salami S, Karimi A. [The effects of diazinon on testicular tissue in adult male rat. A histopathological study]. Urima Medical Journal. 2010; 20(4): 313-19. [Aticle in Persian]
17. Piña-Guzmán B, Solís-Heredia MJ, Quintanilla-Vega B. Diazinon alters sperm chromatin structure in mice by phosphorylating nuclear protamines. Toxicol Appl Pharmacol. 2005 Jan; 202(2):189-98.
18. Jayachandra S, D'Souza UJ. Prenatal and postnatal exposure to diazinon and its effect on spermatogram and pituitary gonadal hormones in male offspring of rats at puberty and adulthood. J Environ Sci Health B. 2014;49(4):271-8. doi:10.1080/03601234.2014.868287
19. Jorsaraei Gh, Beiki AA, Yousefnia Pasha YR, Alizadeh Navaei R. [The in vitro effects of Hinosan and Diazinon on human sperm parameters]. J Babol Uni Med Sci. 2005; 7(2): 30-34. [Article in Persian]
20. Fattahi E, Jorsaraei SG, Parivar K, Moghaddamnia AA. The effects of a single dosage of Diazinon and Hinosan on the structure of testis tissue and sexual hormones in mice. Yakhteh Medical Journal. 2010; 12(3):405-10.
21. Ikeda K, Saito T, Tojo K. Efonidipine, a Ca(2+)-channel blocker, enhances the production of dehydroepiandrosterone sulfate in NCI-H295R human adrenocortical carcinoma cells. Tohoku J Exp Med. 2011;224(4):263-71.
22. Viswanath G, Chatterjee S, Dabral S, Nanguneri SR, Divya G, Roy P. Anti-androgenic endocrine disrupting activities of chlorpyrifos and piperophos. J Steroid Biochem Mol Biol. 2010 May; 120(1):22-9. doi:10.1016/j.jsbmb.2010.02.032
23. Maliji Gh, Jorsaraei SGh, Zabihi E, Fattahi E, Rezaie E, Sohan Faraji A. [Diazinon alters sex hormones, Interferon-gamma, Interleukin-4 and 10 in male Wistar rats]. J Gorgan Uni Med Sci. 2014; 16(1): 22-28. [Article in Persian]
24. Lima CS, Nunes-Freitas AL, Ribeiro-Carvalho A, Filgueiras CC, Manhães AC, Meyer A, et al. Exposure to methamidophos at adulthood adversely affects serotonergic biomarkers in the mouse brain. Neurotoxicology. 2011; 32(6): 718-24.
25. Slotkin TA, Seidler FJ. Transcriptional profiles reveal similarities and differences in the effects of developmental neurotoxicants on differentiation into neurotransmitter phenotypes in Pc12 cells. Brain Res Bull. 2009 Mar; 78(4-5): 211-25. doi:10.1016/j.brainresbull.2008.08.021
26. Proskocil BJ, Bruun DA, Thompson CM, Fryer AD, Lein PJ. Organophosphorus pesticides decrease M2 muscarinic receptor function in guinea pig airway nerves via indirect mechanisms. PLoS One. 2010; 5(5): e10562. doi:10.1371/journal.pone.0010562
27. Selvage DJ, Rivier C. Importance of the paraventricular nucleus of the hypothalamus as a component of a neural pathway between the brain and the testes that modulates testosterone secretion independently of the pituitary. Endocrinology. 2003 Feb; 144(2):594-8.
28. Oswal DP, Garrett TL, Morris M, Lucot JB. Low-dose sarin exposure produces long term changes in brain neurochemistry of mice. Neurochem Res. 2013 Jan;38(1):108-16. doi:10.1007/s11064-012-0896-9
29. Heinrich N, Meyer MR, Furkert J, Sasse A, Beyermann M, Bönigk W, et al. Corticotropin-releasing factor (CRF) agonists stimulate testosterone production in mouse leydig cells through CRF receptor-1. Endocrinology. 1998 Feb;139(2):651-8.
30. Salazar-Arredondo E, de Jesús Solís-Heredia M, Rojas-García E, Hernández-Ochoa I, Quintanilla-Vega B. Sperm chromatin alteration and DNA damage by methyl-parathion, chlorpyrifos and diazinon and their oxon metabolites in human spermatozoa. Reprod Toxicol. 2008 Aug; 25(4):455-60. doi:10.1016/j.reprotox.2008.05.055
31. Leong CT, D'Souza UJ, Iqbal M, Mustapha ZA. Lipid peroxidation and decline in antioxidant status as one of the toxicity measures of diazinon in the testis. Redox Rep. 2013;18(4):155-64. doi:10.1179/1351000213Y.0000000054
32. Banayi M, Mirvaghefi A, Ahmadi K, Ashori R. [The effect of diazinon on histophatological changes of testis and ovaries of common carp (Cyprinus carpio)]. Journal of Marine Biology. 2009; 1(2): 14-25. [Article in Persian]
33. ElMazoudy RH, Attia AA. Endocrine-disrupting and cytotoxic potential of anticholinesterase insecticide, diazinon in reproductive toxicity of male mice. J Hazard Mater. 2012 Mar; 209-10: 111-20. doi:10.1016/j.jhazmat.2011.12.073
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rahimi S, Zamiri MJ, Shariati M, Changizi-Ashtiyani S, Moghadamnia D, Rahimi A. Effect of Diazinon on pituitary-gonadal axis and histological alteration of seminferous tubules in adult rat testis. J Gorgan Univ Med Sci 2016; 18 (1) :23-29
URL: http://goums.ac.ir/journal/article-1-2651-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 1 (3-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4660
Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)