[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aims and Scope
Content Coverage
Conflict of Interest
Publication Ethics
Editorial Board::
Executive Members::
Instruction to Authors::
Peer Review::
Articles Archive::
Current Issue
All Issues
Indexing Databases::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 9, Issue 2 (7-2007) ::
J Gorgan Univ Med Sci 2007, 9(2): 17-23 Back to browse issues page
Frequency of Shiga –toxin producing E.coli (STEC) in patients with hemorrhagic colitis referring to Tehran hospitals
Aslani MM * , Sheshpoli AS , Sadeghiayn S , Alikhani MY
Abstract:   (18054 Views)
Background&Objective: Shiga-toxin producing E. coli (STEC) belonging to several different O serotypes are one of the etiological agent of diarrhea. The STEC strains are considered as an etiological agent for enteritis after non-typhoidal salmonellosis and Campylobacter. They have also been associated closely with the hemolytic uremic syndrome (HUS) and hemorrhagic colitis(HC). The aim of this study was to determine of the frequency of STEC in patients with hemorrhagic colitis referring to Tehran hospitals. Materials&Methods: From March to September 2004, 70 patients with hemorrhagic colitis (Case)an 70 patients with diarrhea (Control) were included in this study. The stx gene was detected by PCR and was used for the determination of STEC strains. Slide agglutination with specific antisera used to detect O serogroup. Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) analysis of the flagellin gene (fliC) was performed for determining their flagellar antigen (H). Results: Two samples (2.9%) from Hemorrhagic colitis cases and 12 samples (17.1 %) from diarrheal cases were positive for STEC. There was no significant correlation between STEC and Hemorrhagic colitis but there was a significant correlation between STEC and diarrhea (p<0.05). STEC isolates O142:H48 serotype was from hemorrhagic colitis cases and O126:H47, O126:H6, O26:H4 and O111:H23 serotypes were from diarrheal cases. These serotypes were not reported in hemorrhagic colitis cases. Conclusion: Our data showed that there was no significant correlation between STEC and hemorrhagic colitis. This could be explained since serotype responsible for hemorrhagic colitis i.e. O157:H7 serotype is not present in Iran.
Keywords: STEC, Hemorrhagic colitis, PCR, Serotyping, O157:H7
Full-Text [PDF 338 kb] [English Abstract]   (18100 Downloads)    
Type of Study: Original Articles | Subject: General

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
MM A, AS S, S S, MY A. Frequency of Shiga –toxin producing E.coli (STEC) in patients with hemorrhagic colitis referring to Tehran hospitals. J Gorgan Univ Med Sci 2007; 9 (2) :17-23
URL: http://goums.ac.ir/journal/article-1-255-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 9, Issue 2 (7-2007) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.04 seconds with 36 queries by YEKTAWEB 4660
Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)