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Effect of aqueous and alcoholic extracts of roots of Ferula gummosa Boiss. on the growth of Pseudomonas aeruginosa
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Salehi M   1, Hashemi Karuie SM2 , Nasrolahi Omran A2 , Mobini M3 , Asghar Hedari M3 |
1- MSc in Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran , mohammadsalehi73@gmail.com
2- Assistant Professor, Department of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
3- MSc in Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran |
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Abstract: (17259 Views) |
| Background and Objective: Ferula gummosa Boiss. (Barije.) contain medical and antimicrobial properties. This study was done to determine the effect of aqueous and alcoholic extracts of roots of Ferula gummosa Boiss. on the growth of Pseudomonas aeruginosa. Materials and Methods: In this laboratory study, the plant was dried in dark place and aqueous, alcoholic extracts of Barije's root, powder were prepared using Soxhlet method. The efficacy of 0.1 dilution of different values of extracts of Ferula gummosa Boiss. on the strain of Pseudomonas aeruginosa (PTCC 1430) were evaluated by disk diffusion, Agar-well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. Results: Pseudomonas aeruginosa was completely resistant to the aqueous extract, and the MIC for the methanol and ethanol extracts was 1.25×104 μg/ml and 6.25×103 μg/ml, respectively. Conclusion: Methanolic and ethanolic extracts of Ferula gummosa Boiss. have antimicrobial activity against Pseudomonas aeruginosa in in-vitro model. |
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| Keywords: Ferula gummosa Boiss, Pseudomonas aeruginosa, MIC, MBC |
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Full-Text [PDF 259 kb]
[English Abstract]
(13131 Downloads)
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Type of Study: Original Articles |
Subject:
Microbiology
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