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:: Volume 14, Issue 2 (6-2012) ::
J Gorgan Univ Med Sci 2012, 14(2): 1-9 Back to browse issues page
Assessment of neurotrophic factors expression and cell proliferation in the coculture of neural and mesenchymal stem cells
Falsafinia Gh (MSc)1 , Ghorbanian MT (PhD) * 2, Lashkarbolouki T (PhD)3 , Elahdadi Salmani M (PhD)3
1- MSc in Developmental Cell Biology, Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
2- Assistant Professor, Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran , ghorbanian@du.ac.ir
3- Assistant Professor, Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
Abstract:   (61801 Views)
Background and Objective: Neurotrophic factors are diffusible polypeptides that have critical roles in survival, proliferation and differentiation of stem cells. This study was done to assess the role of neurotrophic factors (CNTF‎, BDNF, ‎GDN‎F, ‎NT-‎3‎) expression and proliferation rate of neural stem cells (NSCs) in coculture with mesenchymal stem cells (MSCs). Materials and Methods: In this experimental study, NSCs and MSCs were isolated from adult Wistar rat. Initially, NSCs was harvested from temporal lobe after mechanical digestion by a sterile flamed Pasteur pipette and enzymatic digestion with trypsin and Dnase. The cell suspension was cultivated in a flask with DMEM/F12 medium supplemented with 10% FBS 100U/ml Penicillin and 100 mg/ml Streptomycin. To obtain MSCs, bone marrow of femur and tibia bones were flashed out and cultured. MSCs and NSCs‎ cocultured by transwell ‎system in DMEM/F12 medium supplemented with 10% FBS 100U/ml Penicillin and 100 mg/ml Streptomycin. Haemocytometer, immunocytochemistry and RT-PCR methods were performed to identify and evaluate cell proliferation, purity levels and neurotrophic factors expression. Results: There ‎is‎ no differences in NTFs profile of ‎neurotrophic‎ factors expression between ‎coculture ‎group‎ ‎and‎ control ‎NSCs, but interactions between MSCs and NSCs significantly promoted NSCs proliferation (P<0.05). Conclusion: This study showed that coculture of NSCs with MSCs might be prfered in cell-therapy than‎ NSCs.‎
Keywords: Stem cell, Neural cell, Mesenchymal cell, Coculture, CNTF‎, BDNF, ‎GDN‎F, ‎NT-‎3
Full-Text [PDF 925 kb] [English Abstract]   (17977 Downloads)    
Type of Study: Original Articles | Subject: Stem Cells
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Falsafinia Gh (MSc), Ghorbanian MT (PhD), Lashkarbolouki T (PhD), Elahdadi Salmani M (PhD). Assessment of neurotrophic factors expression and cell proliferation in the coculture of neural and mesenchymal stem cells. J Gorgan Univ Med Sci 2012; 14 (2) :1-9
URL: http://goums.ac.ir/journal/article-1-1314-en.html


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Volume 14, Issue 2 (6-2012) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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